供应链柔性系统集成模型

为确定合理的供应链柔性水平，提高供应链绩效，运用系统分析方法，根据供需原理分析了供应链在其生命周期内柔性产生和发展的动力以及供应链柔性的动态特性，需求是拉动供应链柔性动态演变的动力，建立了研发、资源、制造、物流、信息和决策等6个柔性子系统模型，并根据各子系统之间的内在联系建立了供应链柔性系统集成模型。     

云南省发展现代物流业的环境分析和建议

本文论述了云南省发展现代物流业的内部环境、外部环境，并进行内外部环境的SWOT分析，最后根据分析结果对云南省发展现代物流业提出几点建议。     

水泥稳定风积砂作为路面基层材料的试验研究

采用均匀设计的方法来优化试验,以水泥、碎石、风积砂为原材料进行试验研究,得到水泥稳定风积砂的最佳含水量、最大干密度、无侧限抗压强度、回弹模量等指标,并通过SPSS软件进行回归分析,建立回归方程.研究结果表明水泥用量对无侧限抗压强度的影响最大,其次是碎石的用量,风积砂的用量对强度的影响较小.通过铺筑试验路的试验和观测,结果表明,水泥稳定风积砂基层状况良好,且弯沉值满足有关的要求.     

CLONING SEGMENT SPIKE PROTEIN GENE OF SARS-COV AND ITSEXPRESSION IN ESCHERICHIA COLI

Objective Expressing and purifying the seglnent of SARS-CoV spike protein in E. Coli Methods The target gene was obtained by RT-PCR. The PCR product was cloned into pEGM- T Easy Vector, sequencing and double restriction digestion (BamH Ⅰ , Pst Ⅰ) were performed. The target gene was subcloned into PQE30 expression vector. The gene was expressed in the E. coli strain M15 cells induced by IPTG. The protein was purified with a nickel HiTrap chelating metal affinity column. Results The recombinant expression plasmid was successfully constructed and the protein was well expressed in E. coli strain M15 cells. The ideal pure protein was obtained by purification. Western blotting analysis suggested the protein could act with the convalescent sera of lab confirmed SARS patients. Conclusion The segment of SARS-CoV spike protein was well expressed and purified, and can be applied in diagnosis and immunological research of SARS.     

桥梁现场常见病害及检查

建于上世纪60、70年代的公路桥梁,现在大多带病服务于现代交通运输中,因此,对这些桥梁进行病害检查就显得十分重要。桥面系、上部构造、支座、墩台及基础是病害检查的重要部位。文中指出了这些部位的常见病害,并分析了产生这些病害的原因,为桥梁的养护决策提供依据。     

仿紧集上新型抽象广义双拟变分不等式

笔者在本文应用文献[1]中得到的极大极小不等式研究了仿紧集上新型抽象广义双拟变分不等式解的存在性问题.     

葡萄糖、胰岛素和尿酸对猪近端肾小管上皮细胞株血红素氧合酶-1的影响

目的观察不同浓度葡萄糖、胰岛素和尿酸的培养条件下猪近端肾小管上皮细胞株(LLC-PK1)血红素氧合酶(HO-1)活性的改变,以探讨代谢相关因素在细胞水平对肾小管上皮细胞的影响。方法以LLC-PK1为研究对象,观察葡萄糖浓度为5、10、22、33 mmol/L,胰岛素浓度为0、10-9、10-8、10-7mol/L,尿酸浓度为0、0.1、0.2、0.4 mmol/L培养48 h条件下,LLC-PK1细胞HO-1活性的改变。结果0.1 mmol/L0、.2 mmol/L和0.4 mmol/L尿酸刺激48 h后,LLC-PK1的HO-1活性增加,并呈剂量依赖关系,而上述浓度的葡萄糖和胰岛素刺激48 h对LLC-PK1的HO-1活性无影响。结论一定浓度的尿酸可使LLC-PK1的HO-1活性升高,提示尿酸在细胞水平对肾小管上皮细胞的氧化应激过程产生影响。     

单壁钢围堰在大潮差桥梁桩基加固中的应用

桥梁水中桩基在江、河、海水的日益冲刷下出现混凝土剥落、钢筋外露锈蚀,导致桩基缩径,极大地威胁着桥梁结构的安全性,这就需要对桩基进行维修加固。结合《大潮差桥梁桩基钢围堰施工技术研究》(S20130013)和温州市东瓯大桥桩基加固工程实例,介绍单壁钢围堰技术在处理水下桩基础破损露筋等质量问题中的应用,阐述了钢围堰加固桩基施工方案的实施和施工中应注意的几个关键技术。     

双浮体波浪能装置的振荡和能量转换特性研究（英文）

In this study, we investigated the hydrodynamic and energy conversion performance of a double-float wave energy converter(WEC) based on the linear theory of water waves. The generator power take-off(PTO) system is modeled as a combination of a linear viscous damping and a linear spring. Using the frequency domain method, the optimal damping coefficient of the generator PTO system is derived to achieve the optimal conversion efficiency(capture width ratio).Based on the potential flow theory and the higher-order boundary element method(HOBEM), we constructed a threedimensional model of double-float WEC to study its hydrodynamic performance and response in the time domain. Only the heave motion of the two-body system is considered and a virtual function is introduced to decouple the motions of the floats. The energy conversion character of the double-float WEC is also evaluated. The investigation is carried out over a wide range of incident wave frequency. By analyzing the effects of the incident wave frequency, we derive the PTO's damping coefficient for the double-float WEC's capture width ratio and the relationships between the capture width ratio and the natural frequencies of the lower and upper floats. In addition, it is capable to modify the natural frequencies of the two floats by changing the stiffness coefficients of the PTO and mooring systems. We found that the natural frequencies of the device can directly influence the peak frequency of the capture width, which may provide an important reference for the design of WECs.     

地连墙基坑顺作开挖的动态规划研究

基坑开挖是一个时间和空间不断变化的过程,其中存在开挖参数的优化问题,但是缺乏有效的规划方法。本文针对地连墙基坑,引入响应面-枚举法研究其顺作开挖参数的规划。并以云南某基坑为例,规划顺作基坑开挖参数,以此验证响应面-枚举法的可行性。