公路工程主体设计应考虑的环保措施

本文通过公路工程主体设计时对生态环境、水环境、声环境、空气环境以及社会环境等相关环保措施的系统阐述,初步对目前公路工程建设中主体设计时所采用的环保措施进行初步的探讨,为相关技术人员进行工程设计时提供相应的参考。     

Improving the efficiency of demand-responsive paratransit services

State agencies responsible for ADA-eligible paratransit services are increasingly under pressure to contain costs and maximize service quality. Many do not operate vehicles themselves; instead, they contract out the provision of services. Contractors are paid for each hour of service. They are responsible for hiring crew, forming routes, dispatching, and operating and maintaining agency-owned vehicles. In the Twin Cities of Minneapolis and Saint Paul, Metro Mobility, the agency responsible for providing paratransit services, requires contractors to use agency-approved software for booking trips dynamically and sets parameters that guide contractors’ practices. Customer trips booked in this fashion may not utilize capacity in the most efficient manner. Therefore, beginning with the daily trip schedules generated by the software, this paper proposes two approaches for improving the efficiency of paratransit operations and estimates the benefit of using these approaches via experiments that utilize Metro Mobility data. The first approach re-optimizes routes developed at the end of each day. The second approach evaluates the benefit to state agencies of selectively using non-dedicated service providers such as taxis. Both approaches are tested on actual data obtained from Metro Mobility. The study shows that a conservative estimate of savings from re-optimization would be approximately 5% of Metro Mobility’s operating costs. Savings from the use of taxis are smaller and in the range of hundreds of dollars per day.     

罗兰C TOA-ASF测量系统设计与实现

附加二次相位因子（ASF）是影响罗兰C导航/授时系统精度的主要因素。进行ASF修正主要有理论预测法和实测法两种。针对罗兰C到达时间ASF实测修正的需要,本文给出了ASF测量系统的测量原理和测量系统组成,详细介绍了测量系统的设置、软硬件设计。车载试验表明,该测量系统的设计是成功的,能够满足ASF实测的需要。     

狭小空间底模支设施工技术

主要探讨了如何有效地在100 mm的狭小空间进行底模的支设,并保证现浇混凝土轨道梁的质量,同时底模必须方便顺利地取出,确保车站结构的使用安全性。     

浅谈丹东市防洪工程体系

介绍了丹东市防洪工程现状,对防洪工程体系及存在问题进行了分析,有关经验可供相关专业人员参考。     

民生警务背景下电动自行车治理困境与应对策略

大量的电动自行车违法行为给交通管理增加了繁重的压力的同时,也给民生警务改革、构建和谐警民关系带来了难题。在此背景下,文章首先阐述了新修订的《浙江省电动自行车管理条例》主要内容,接着分析了目前电动自行车治理存在民众对新《条例》认知不足、违法者不认同执法、电动自行车驾驶员安全意识不高和违规电动自行车生产销售等问题,最后有针对性地从宣传引导、加强执法协作和强化监管等方面提出了相应的对策建议。     

浅析汽车维修新技术中发动机故障检测及维修技术

随着我国社会发展进步,汽车已经成为人民生活中不可或缺的一部分,而汽车运行过程中难免会发生故障,其中发动机故障的影响尤为严重。从简述汽车维修新技术的重要作用入手,进一步分析汽车发动机故障的检测方法,探究汽车维修新技术在发动机异响、发动机油样、发动机启停及发动机冷却系统故障检测和维修的途径。     

浅析分流锥冷却系统的改进

在市场竞争日趋激烈的今天,企业要生存、求发展,要想取得良好的经济效益,就必须以适销对路的产品占领市场,以质优价廉的产品去巩固和拓展市场,因此说,企业开发人员的创造力是企业发展的关键所在。将创造性思维和方法运用于实际工作中,对分析和解决生产中出现的难点问题,提高工作效率都具有十分重要的意义。南海中南铝车轮制造有限公司生产的A型号铝合金车轮产品,由于安装盘部位疏松(组织不致密)缺陷,产品的合格率一直徘徊在70%左右,废品发现时,已经过了合金熔炼、毛坯铸造、热处理、机加工等工序,所以造成了相当大的损失,虽然采取过一系列相应措施加以改进,但未找到问题的症结,效果也不理想。     

Preparation of recombinant firefly luciferase by a simple and rapid expression and purification method and its application in bacterial detection

A simple and rapid expression and purification method of recombinant firefly luciferase was developed for bacteria detection. A modified luciferase gene from North American firefly Photinus pyralis was cloned into pET28a expression vector and the recombinant protein was produced in Escherichia coli BL21. The recombinant luciferase, equipped with a polyhistidine affinity tag, was purified by immobilized metal ion affinity chromatography (IMAC). The approach generated an abundant expression and an efficient purification of a recombinant luciferase with final yield 1.995mg/L of cell culture. Experiments on the recombinant luciferase also showed that the relative light units (RUL) of the enzyme were 5.8×108, and the specific activity was 2.9×1010 RLU/mg. By applying adenosine triphosphate (ATP) bioluminescence to detection of the coin bacteria using the recombinant protein, the ATP content of bacteria was 9.48×10-16mol/mL, and was identical to the bacteria counts (4500CFU/mL) in order of magnitude. Taken together, our results provided a simple and efficacious method of the preparation of recombinant luciferase, which could be applied in the determination of bacteria via ATP bioluminescence.