涎腺肿瘤组织中人乳头瘤病毒DNA的检测

为探讨腺上皮起源的涎腺良、恶性肿瘤与人乳头瘤病毒（Ｈｕｍ ａｎ ｐａｐｉｌｌｏ ｍ ａｖｉｒｕｓ, ＨＰＶ）感染的关系,应用聚合酶链反应技术（ＰＣＲ）,采用共有引物检测４４例涎腺肿瘤组织中的ＨＰＶ ＤＮＡ的存在状况。１０ 例正常涎腺组织作为对照。结果显示：肿瘤组织中ＨＰＶ ＤＮＡ 阳性检出率达７０．４５％ （３１／４４）,其中恶性肿瘤 ＨＰＶＤＮＡ 阳性率为７７２７％ （３１／４４）;良性肿瘤ＨＰＶＤＮＡ阳性率为６３６３％ （１４／２２）;而正常对照组织中ＨＰＶ ＤＮＡ阳性率仅为１０％ （１／１０）。提示：涎腺肿瘤的发生可能与ＨＰＶ感染有关     

青岛市汇泉湾广场改造市政工程设计探讨

该文针对百年汇泉湾广场的特殊地理位置及深厚的文化内涵,介绍了广场改造工程的道路交通组织设计,阐述了城市道路景观工程、排水工程及地下人行通道工程的设计思路与方法,并对设计过程进行了总结分析,以供相关工程参考。     

Dynamic Performance of a Bulkhead Flat Car

The dynamic performance of a flat car is studied herein. The performance indices include roll angles, lateral accelerations, center plate loads, side bearing loads, wheel loads and spring deflections. These variables are maximum when the car is running at its critical speed, corresponding to either the rock and roll, or the bounce mode. The Association of American Railroads' (AAR) Flexible Carbody Model was used. The input for vertical track irregularities used in the simulation was generated from published spectra for U.S. Federal Railroad Administration (FRA) Class 4 track. After studying the car's performance with various column loads and spring suspensions, it was found that the most commonly used column load of 4,000 lbs. (17.8 kN) should be used. The spring suspension used in the original car design should also be adopted, in order to avoid spring bottoming.     

神经网络信息融合方法在电控发动机故障诊断中的应用研究

利用虚拟仪器技术研制了数据采集与处理系统，可对发动机进行实时数据的采集、历史数据和暂态数据的显示，以及数字滤波、频谱分析、波形显示等方面的数据后处理。以电控发动机常见的故障现象——怠速不稳为例，研究了一种特征层信息融合方法——基于神经网络的信息融合技术在电控发动机故障诊断中的应用。     

The development of safe riding guidelines for young riders – A case study of Phu Yen,Vietnam

Phu Yen is a small province located in the central part of Vietnam and is suffering from a tremendous number of traffic casualties, compared with other cities in Vietnam and developed countries. Young riders take a large proportion, accounting for 47.6% in total traffic crashes. To resolve this issue, the present study aims to (i) analyze young riders' behavior and their attitudes towards traffic safety and to (ii) propose a procedure to develop a book of safe riding guidelines to reduce their involvement in dangerous situations. A set of 73 questions in the questionnaire survey have been conducted to determine the risk-taking behavior and attitude towards traffic safety of 300 individuals, who are using bicycles, e-bikes, mopeds, scooters, and motorcycles less than 150 cc. The statistical results indicate that there are significant differences among riding behavior and attitudes of respondents towards traffic safety regarding the regional factor (rural, suburb, and urban), the demographic factor (gender and age), and the travel distance factor. The guidelines designed based on the questionnaire survey are used to alter adolescents' riding behavior and attitudes and so help them recognize, avoid, and handle risks in demanding traffic situations. An analysis of the before and after the traffic safety training workshop to evaluate the effectiveness of the proposed guidelines indicated that there was a significant increment in the perceived risks of young riders after training them by using this book.     

运用RCPMIS进行物资管理的实践

物资管理子系统是铁路建设工程管理信息系统(RCPMIS)一个子模块。介绍了RCPMIS在贵广铁路建设中的实践,进一步验证了先进的信息化工程管理手段可以有效地提高人员工作效率,为全过程、全方位、高效的工程物资管理奠定基础的道理。     

DIG－DNA探针检测宫颈癌组织中人乳头瘤病毒6、11、16、18型DNA

用地高辛配基（Ｄｉｇｏｘｉｎｇｅｎｉｎ）分别标记，制备ＨＰＶ－６、ＨＰＶ－１１、ＨＰＶ－１６及ＨＰＶ－１８的ＤＮＡ探针，应用核酸杂交技术，对３７例宫颈癌活检组织ＤＮＡ作了检测，各型ＨＰＶＤＮＡ的检出率为：ＨＰＶ－６０％（０／３７）、ＨＰＶ－１１２．７（１／３７）、ＨＰＶ－１６６７．５％（２５／３７）、ＨＰＶ－１８１３．５％（５／３７），联合应用ＨＰＶ－１６和ＨＰＶ－１８探针共同检测结果为８１％（３０／３７）。结果显示：我国宫颈癌的发生与ＨＰＶ感染密切相关，联合应用高危型ＨＰＶ探针可提高检出率并可省时、简便。ＤＩＧ标记探针因具特异、敏感、安全和简便诸特点，宜推广应用。     

用分子杂交法检测妇女宫颈疾患中的HPVDNA

为了探讨人乳头瘤病毒（ＨＰＶ）与宫颈癌之间的相关性，我们收集了全国宫颈癌高发县之一的略阳县１２例宫颈癌，１２例宫颈间变，３６例宫颈Ⅱ～Ⅲ度糜烂患者的组织标本，经抽提ＤＮＡ以后，以［ａ－３２Ｐ］ｄＡＴＰ标记ＨＰＶ１６、１８型ＤＮＡ进行打点杂交。结果为宫颈癌ＨＰＶ１６阳性６例，ＨＰＶ１８阳性１例。宫颈间变ＨＰＶ１６阳性４例，ＨＰＶ１８阳性１例。ＨＰＶ１６阳性率分别为５０％和３３．３％，ＨＰＶ１８阳性率为８．３％。宫颈糜烂Ⅱ～Ⅲ度的患者组织ＤＮＡ与ＨＰＶ１６、ＨＰＶ１８混合杂交阳性１７例，阳性率为４７．２％。     

高职高专电子商务专业双语教学刍议

本文首先从社会需求、专业改革、人才培养等角度论述了高职高专电子商务专业双语教学的必要性,并从语言学的角度论述了其理论基础。然后分析了适合我国高职高专电子商务专业双语教学的教学模式,最后分析了存在的问题及对策。     

玄武岩纤维水泥砂浆的力学性能研究

以有机聚丙烯纤维为对比,进行了无机玄武岩纤维水泥砂浆的抗压、抗折、抗拉伸及抗弯系列力学性能试验研究。研究结果表明：在最佳掺量下,玄武岩纤维水泥砂浆的各种力学性能优于聚丙烯纤维水泥砂浆;玄武岩纤维对水泥浆体早期具有显著的增强作用,但降低了水泥砂浆的28d强度;掺入玄武岩纤维可以增加砂浆的韧性,对砂浆的抗拉强度改善起到了一定作用;玄武岩纤维对砂浆的抗弯破坏强度改善不显著,但明显增大了相同荷载下试件的挠度。