幽门螺杆菌尿素通道蛋白基因UreI载体的构建、融合表达及纯化

目的构建幽门螺杆菌(H.pylori,Hp)尿素通道蛋白基因UreI的融合表达载体,并在E.coliBL21中表达,为进一步研究UreI的功能奠定基础。方法利用分子克隆技术以Hp DNA染色体为模板,扩增Hp UreI基因片段,将目的基因UreI与载体pET32a( )分别经kpnⅠ和HindⅢ双酶切,纯化,连接。筛选阳性重组载体,转化大肠杆菌BL21,以IPTG诱导表达pET32a( )/UreI融合蛋白。表达产物经Ni-NTA琼脂糖树脂纯化后,用SDS-PAGE及Western blot分析。结果经酶切、测序分析表明,插入的基因片段为Hp UreI编码基因,由585 bp组成,与GenBank相应菌株HP AM417 609序列完全一致,无碱基突变。经SDS-PAGE分析显示重组质粒pET32a( )/UreI在原核细胞中得到了高效融合表达,其重组融合蛋白分子量为130 ku,经Ni-NTA琼脂糖树脂纯化后,其纯度达95%以上,Western blot分析显示,该重组融合蛋白可被6-His鼠抗单克隆抗体所识别,具有良好的反应原性。结论成功地克隆和构建了原核表达载体pET32a( )/UreI,并在原核细胞中得到了高效融合表达。     

Information System for Land-Use Planning and Management

In order to maintain the overall social interest in land use and improve the level of land administration, an information system for land-use planning and management (ISLUPM) was established, which is composed of presentation layer, business logic layer and data layer in the general structure. The application support platform of the ISLUPM, built based on COM, COM and .NET standard components, includes data engine, data management, assemblies, components management, operation management, and interface. Then, an elaboration was made on major functions of the ISLUPM, such as planning revision scheme, planning operation flow, digital processing, thematic analysis and inquiry, and preparation of the chart of reserved land resources. The developed system has been successfully applied to the land-use planning and management work of Longquanyi District, Chengdu, China. It may provide a reference for development of geographic information system (GIS) for land and resources.     

公路养护现代化水平多级模糊评估

科学、高效的现代化公路养护作业与养护管理方法可使公路在使用年限内经常保持完好状态,从而最大限度地发挥公路的运输经济效益.根据公路养护的特点及公路养护现代化的内涵建立了公路养护现代化评估指标体系,根据模糊数学理论构建了公路养护现代化水平多级模糊评估模型,利用该模型可以对一个地区的公路养护现代化水平进行衡量和评估,从而找出存在的不足,为公路养护现代化建设起到引导作用.示例研究表明,该方法可行.     

半柔性路面复合材料抗压回弹模量研究

通过对基体混合料设计空隙率25%的复合材料进行室内试验得到相应的抗压回弹模量,并与实体路面工程实测弯沉反算得到的抗压回弹模量进行比较,提出了确定复合材料抗压回弹模量的改进方法,由此计算出基体混合料空隙率分别为20%和30%对应的复合材料回弹模量,并确定了相应的取值范围,最后通过回归得到复合材料回弹模量与基体混合料空隙率之间的关系式,由此可确定任意空隙率的复合材料回弹模量,为半柔性复合路面设计提供了重要参数。     

交通环境变化下的交通与经济互动性分析

交通和经济是区域的两大职能因素,它们是相互影响并且相互制约的。交通环境的变化将对区域经济产生重要的影响,同时经济发展也将进一步地刺激交通发展。以宜昌市为例,在分析宜昌交通环境变化及交通和经济发展的数据基础上,从对交通和经济相互影响的两个方面的分析,探讨了交通与经济的互动性。     

基于HLA的船用压水堆运行控制仿真实现

仿真系统在HLA规范下,利用VR-Link/RTI构建分布式网络平台,在VC 程序中对MATLAB和Vega进行联邦成员的改写实现了船用反应堆主回路系统的虚拟仿真.HLA技术的可扩充性和复用性使基于HLA的船用反应堆运行控制仿真能够扩充并能够很好地复用到船用核动力系统虚拟仿真乃至整船的航行控制虚拟仿真中去,是其重要的基础工作.     

Effect of ABCA1-V771M polymorphism on plasma lipid levels and its relationship with coronary atherosclerotic heart disease

Objective To explore the risk association of ABCA1-V771M polymorphism with coronary heart disease (CHD) in Hart nationality in Northwest of China. Methods With case-control study, ABCA1-V771M polymorphism was detected in 204 unrelated Hart nationality people in Northwest of China, and all the subjects by coronary angiography were grouped into 106 cases and 98 controls. The genotypes and alleles frequency distribution of ABCA1-V771M polymorphisms were analyzed by PCR-RFLP analysis, and the clinical statistics of serum lipids were compared and its effects of ABCA1-V771M polymorphism on the plasma lipid levels and coronary atherosclerotic heart disease were analyzed. Results The genotypic frequencies of ABCA1-V771M polymorphism matched well under Hardy-Weinberg equilibrium (P>0.05), V and M allelic frequencies were 33.3% and 66.7%. In comparison with VV VM genotype carriers, MM genotypes carriers had much lower plasma levels of HDL-C (P<0. 001) and much higher plasma levels of TG (P<0. 05). M allelic frequency in CHD group was significantly higher than V allelic frequency (P<0. 05). M allele was related with more severity of atherosclerosis in the coronary artery than V allele (P<0.05). However, there was no obvious difference in the incidence of AMI among carriers with three genotypes of ABCA1-V771M polymorphism (P>0.05). Conclusion ABCA1-V771M polymorphism was not only associated with the plasma levels of HDL-C and TG, but also related to the susceptibility and severity of coronary atheroselerotic heart disease. Moreover, M771 allele appeared to be atherogenie among Han population in Northwest of China.     

城市快速道路规划建设若干技术问题初探

研究探讨城市道路网体系基本要求,城市快速路规划设计关键技术要领,快速道路几何形式及高架路建设利弊因素等.为城市快速路规划建提供参考.     

关于高速公路交通安全设施的设置

交通安全设施对减轻事故的严重度,排除各种纵、横向干扰,提高道路服务水平,提供视线诱导,增强道路景观等起着重要的作用,属于道路的基础设施.     

现代汽车新材料技术应用

材料技术的进步,降低了车辆质量,改善了燃料经济性,并减少了车辆制造成本。近年来,现代汽车普遍采用碳纤维增强材料、陶瓷碳素复合材料以及纳米材料等最新技术,介绍纳米汽油、纳米润滑剂、纳米塑料、碳纳米管、纳米界面材料、新型汽车尾气检测装置等应用于汽车的纳米技术。