10kV自闭贯通线单相接地故障判断方法的研究

针对目前铁路10kV自闭、贯通电力线路发生单相接地故障在判断查找中存在的问题及危害进行了分析,提出了解决问题的办法,文章对新研制的故障判断系统进行了详细的介绍,并进行了大量的现场试验,取得了各种情况下的试验数据,确定了数值范围,取得了较好的效果。     

Accelerated Service Life Testing of Automotive Vehicles on a Test Course

The accelerated service life testing of automotive vehicles for durability to road roughness induced dynamic loads is often accomplished in the laboratory using road roughness simulation facilities [1-5]. However, such tests can also be accomplished by a carefully designed field operation on a test course [6], where both the speed of the vehicle and the roughness of die test course become variables that control the degree of the test acceleration. Field tests are generally harder to control than laboratory tests, but offer a greater degree of realism since the vehicle is fully operational during the test exactly as it will be in service. This paper formulates the criteria for accelerated service life tests on a test course, evaluates the assumptions that must be enforced to obtain valid results, and explores the sensitivity of the results to the critical test parameters, namely, the vehicle speed and the road roughness severity of the test course relative to the service environment.     

Analytical extension of the effective axle characteristics concept for the development of a structured chassis design process

The lateral vehicle dynamics is defined by the effects of side forces at the front and rear axle. These forces are caused by the slip and camber angle at the individual tyres, which are results of the kinematics and compliances of the chassis. This paper extends the approach of the effective axle characteristics by Paceyka to the analytical expression of the axle cornering stiffness and the axle relaxation behaviour with the aim of the development of a chassis design process as it applies in the early design stage. The obtained expression is integrated into a single track model and validated against a full nonlinear two-track model. By this means of these analytical expressions for the axle cornering stiffness and the axle relaxation behaviour it is possible to directly calculate and analyse the effective slip angles for linear quasi-static and dynamic driving manoeuvres.     

Semi-active control of tracked vehicle suspension incorporating magnetorheological dampers

In past years, the application of magnetorheological (MR) and electrorheological dampers in vehicle suspension has been widely studied, mainly for the purpose of vibration control. This paper presents theoretical study to identify an appropriate semi-active control method for MR-tracked vehicle suspension. Three representative control algorithms are simulated including the skyhook, hybrid and fuzzy-hybrid controllers. A seven degrees-of-freedom tracked vehicle suspension model incorporating MR dampers has been adopted for comparison between the performance of the three controllers. The model differential equations are derived based on Newton's second law of motion and the proposed control methods are developed. The performance of each control method under bump and sinusoidal road profiles for different vehicle speeds is simulated and compared with the performance of the conventional suspension system in time and frequency domains. The results show that the performance of tracked vehicle suspension with MR dampers is substantially improved. Moreover, the fuzzy-hybrid controller offers an excellent integrated performance in reducing the body accelerations as well as wheel bounce responses compared with the classical skyhook and hybrid controllers.     

Geometric-based tyre vertical force estimation and stiffness parameterisation for automotive and unmanned vehicle applications

Advanced empirical, and physical-based tyre models have proven to be accurate for simulating tyre dynamics; however, these tyre models typically require expensive and intensive tyre parameterisation. Recent research into wheeled unmanned ground vehicles requiring vertical force analysis has shown good results using a simple linear spring model for the tyre which demonstrate the continued use for simple tyre models; however, parameterisation of the tyre still remains a challenge when load test equipment is not available. This paper presents a cost-effective tyre vertical stiffness parameterisation procedure using only measured tyre geometry and air pressure for applications where high-fidelity tyre models are unnecessary. Vertical forces calculated through an air volume optimisation approach are used to estimate tyre vertical stiffness. Nine tyres from the literature are compared to evaluate the performance of the vertical force estimation and stiffness parameterisation algorithms. Experimental results on a pair of ATV tyres are also presented.     

Adaptive control of the shifting process in automatic transmissions

This paper focuses on the way of keeping shift quality of automatic transmissions consistent in mass production and with mileage accumulation. We investigate the main factors influencing the consistency of shift quality. Test results show that the torque to pressure (T2P) and pressure to current (P2I) characteristics of shifting elements are easily affected. A simulation model of an 8-speed automatic transmission is established to simulate the dynamic process of clutch-to-clutch shift. Simulation results demonstrate that the change of T2P and P2I characteristics has a significant influence on shift quality. In order to compensate for the influences, we develop two adaptive control strategies, i.e., the adaptive control strategies for torque phase and inertia phase. They make use of the measured speed information and time information to evaluate shift quality. Then the control parameters are tuned to adapt to the change of T2P and P2I characteristics. Vehicle tests verify that the developed adaptive control strategies are effective to keep shift quality consistent in mass production and with mileage accumulation.     

用于深埋圆形盾构隧道地震效应分析的2种拟静力解析解的对比研究

对比分析深埋圆形盾构隧道地震效应分析常用的2种拟静力解析解,给出2种拟静力解析解法的弯矩比值、轴力比值的计算公式,研究地层弹性模量和泊松比与弯矩比值、轴力比值的关系,以及界面滑移和不滑移条件下对弯矩比值、轴力比值的影响;建立等效刚度数值模型,通过数值模拟验证这2种拟静力解析解的可靠性。结果表明:界面不滑移时,由这2种拟静力解析解获得的地震附加弯矩较为接近,但附加轴力差异较大;地层弹性模量、泊松比和界面条件对地震附加弯矩影响较小,但对地震附加轴力影响较大;由这2种拟静力解析解法与等效刚度数值解法所得的弯矩、轴力分布规律和极值位置基本一致。推荐在常规深埋圆形盾构隧道抗震设计时优先采用基于薄壁圆柱壳理论的拟静力解析方法。     

THE HUMORAL AND CELLULAR IMMUNE RESPONSES INDUCED BY HPV18L1-E6/E7 DNA VACCINES IN MICE

Objective To construct eukaryutic expression vector of HPV18 L1- E6, E7 chimeric gene and examine the humorul and cellular immune responses induced by this DNA vaccines in mice. Methods The C-terminal of major rapsid protein L1 gene and mutant zinc finger domains of early E6/7 oncogenes in HPV18 were integrated and inserted into eukaryotic expression vector pVAX1 to generate vaccines pVAX1-L1E6Mxx, ETMxx. CHO cells were transiently transfected with the individual construct. Target protein expressions in the lysate of the transfected cells were measured by ELISA and immunocytochemistry After BALB/c mice were vaccinated with various recombinant plusmids（pVAX1- L1-E6M3 or pVAX1-L1-E7M3 ） and immunie adjuvants （pLXHDmB7-2 or LTB） through different administration routes （intramuscular or intranasal） , the great cellular immune responses were produced us revealed by delayed-type hypersensitivity （DTH） and lymphocyte proliferation, and the expression of IL-4 and IFN- 7 cells in CD4^＋ and CD8^＋ subpopulations. Results The highly efficient expression of pVAX1-L1E6Mxx, E7Mxx vector in host eukaryotic cells were demonstrated both by ELISA and immunocytochemistry. The level of specific serum IgG against HPV in experiment groups mice was much higher than that of control group, and intranuscular immunization group had the highest antibody level. Intramuscular immunization groups were superior to intranasal immunization groups in DTH response, splenocyte proliferation and CD8^＋ IFN-γ^＋ cells number, but CD4^＋ IL4^＋ cell number was higher in intranasul immunization groups. The immunization groups using pLXHDmB7.-2 as adjuvant were superior to other groups in immunorespouse. Conclusion These DNA vaccines produce remarkable cellular and humorul immune responses in the mouse and may provide us prophylatic and therapeutic candidates for HPV induced cancer treatment.     

应用免疫组织化学方法评价组织芯片的可靠性

目的探讨组织芯片技术在免疫组化试验中的可靠性和提高组织芯片可信性的方法。方法选择82例人乳腺癌标本石蜡蜡块,利用传统制片技术和自行研制的专用器具分别制作常规石蜡切片和组织芯片,采用免疫组化技术检测雌激素受体(ER)、孕激素受体(PR)的表达。结果采用自行研制的专用器具制备组织芯片,所得样本的可分析率均在92.9%以上,且随着对同一标本取材数量的增加,其可分析标本率也明显上升。采用传统方法检测的82例乳腺癌标本中ER和PR的阳性率分别为61%和58.5%;采用组织芯片技术,一式一份取材,其阳性率分别为51.9%和50.0%,一式两份方式取材,其阳性率分别为53.8%和53.8%,采用一式三份方式取材,其阳性率分别为57.1%和60.7%,且三种不同取材方式的检测结果与传统制片法之间并无统计学差异(P>0.75)。结论在采用统一制备标准并保证组织芯片质量的前提下,挖取直径1.5mm的组织样本制备组织芯片,尽管组织芯片技术与传统方法检测的结果的一致率仍随着对同一标本取材次数的增加而提高,但是三种不同取材方式的检测结果之间并无统计学差异。因此,一式一份取材制备组织芯片完全可替代传统制片技术用于免疫组织化学乃至原位杂交、荧光原位杂交技术的研究,且可保证组织芯片的可信性和高通量特征。