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221.
Si Lüsheng 《西安交通大学学报(英文版)》2006,18(1):44-48,77
Objective To construct eukaryutic expression vector of HPV18 L1- E6, E7 chimeric gene and examine the humorul and cellular immune responses induced by this DNA vaccines in mice. Methods The C-terminal of major rapsid protein L1 gene and mutant zinc finger domains of early E6/7 oncogenes in HPV18 were integrated and inserted into eukaryotic expression vector pVAX1 to generate vaccines pVAX1-L1E6Mxx, ETMxx. CHO cells were transiently transfected with the individual construct. Target protein expressions in the lysate of the transfected cells were measured by ELISA and immunocytochemistry After BALB/c mice were vaccinated with various recombinant plusmids(pVAX1- L1-E6M3 or pVAX1-L1-E7M3 ) and immunie adjuvants (pLXHDmB7-2 or LTB) through different administration routes (intramuscular or intranasal) , the great cellular immune responses were produced us revealed by delayed-type hypersensitivity (DTH) and lymphocyte proliferation, and the expression of IL-4 and IFN- 7 cells in CD4^+ and CD8^+ subpopulations. Results The highly efficient expression of pVAX1-L1E6Mxx, E7Mxx vector in host eukaryotic cells were demonstrated both by ELISA and immunocytochemistry. The level of specific serum IgG against HPV in experiment groups mice was much higher than that of control group, and intranuscular immunization group had the highest antibody level. Intramuscular immunization groups were superior to intranasal immunization groups in DTH response, splenocyte proliferation and CD8^+ IFN-γ^+ cells number, but CD4^+ IL4^+ cell number was higher in intranasul immunization groups. The immunization groups using pLXHDmB7.-2 as adjuvant were superior to other groups in immunorespouse. Conclusion These DNA vaccines produce remarkable cellular and humorul immune responses in the mouse and may provide us prophylatic and therapeutic candidates for HPV induced cancer treatment. 相似文献
222.
Jan Pfluger Rene Savelsberg Thomas Hülshorst Stefan Pischinger Jakob Andert 《International Journal of Automotive Technology》2018,19(4):643-650
In this paper, a Field Programmable Gate Array (FPGA) was used to implement a real-time cylinder pressure analysis. The goal of the project was to improve the accuracy of calculated heat release and center of combustion calculations to enhance the precision of engine control functions. Compared to today’s real-time pressure analysis systems, several additional physical effects were taken into account for this objective. The wall heat transfer was calculated based on the approach published by Hohenberg. A chemical equilibrium with six substances was assumed for the mixture composition and a real-time calculation method was developed. Furthermore, a two-zone model was adapted and implemented for this realtime analysis. The validation of the results and the rating of the improvement in precision were based on GT-SUITE simulation results as an offline reference tool. Compared to state-of-the-art analysis systems, it was possible to reduce the average error of the center of combustion position from 1.6° to 0.5° crank angle (CA) by taking the investigated effects into account. Moreover, it was possible to significantly reduce the time required for the calculation from one complete combustion cycle to 0.2°CA at an engine speed of 3,000 rpm by using a continuous calculation method on the FPGA. This led to an additional improvement of the ability to control the engine, especially under highly dynamic operation conditions. 相似文献
223.
为解决软弱破碎围岩地层隧道松动圈测试的难题,分析现有松动圈测试技术现状,以全长黏结型玻纤锚杆为载体,提出一种适用于破碎软岩隧道的围岩松动圈测试技术。该测试技术以锚杆微应变变化趋势为基础,结合锚杆中性点理论,可大致确定围岩松动圈范围。将该技术应用在大(理)临(沧)铁路杏子山隧道破碎炭质板岩大变形地段进行验证,结果表明: 全长黏结型玻璃纤维锚杆在松动区可与软弱破碎围岩有效协调变形,锚杆微应变变化趋势与中性点理论相符,采用锚杆微应变变化趋势表征围岩松动圈范围合理可行。同时该技术测试精度可通过调整沿锚杆长度粘贴应变片的密度来控制,有效弥补传统物探松动圈测试技术对岩体要求高的问题,在软弱破碎围岩地层中具有较好的操作性和适用性。 相似文献
224.
RMS Values for Force, Stroke and Deflection in a Quarter-car Model Active Suspension with Preview 总被引:1,自引:0,他引:1
A. G. Thompson C. E. M. Pearce 《Vehicle System Dynamics: International Journal of Vehicle Mechanics and Mobility》2003,39(1):57-75
Summary In the case of a quarter-car vehicle model incorporating an active suspension, the addition of preview to the system necessitates finding new techniques for the direct computation of rms values for control force, suspension stroke and tyre deflection on a random road. Here we obtain these rms values from the performance index components as derived analytically by means of matrix operations requiring the solution of Lyapunov equations and the use of similarity transformations. Some numerical examples and MATLAB programs are included. 相似文献
225.
C. L. Myung K. H. Choi I. G. Hwang K. H. Lee S. Park 《International Journal of Automotive Technology》2009,10(2):161-166
In SI engines, valve events have a major influence on volumetric efficiency, fuel economy and exhaust emissions. Moreover,
swirl and tumble motions in the intake charge also improve combustion speed and quality by stratifying the mixture as well
as intensifying the mixing rate of air and fuel. This paper investigates the behaviors of an engine and the combustion phenomenon
for various intake valve timings and intake charge motions using CVVT system and port masking schemes. Test condition includes
a part load and a cold idle condition inclusive of a cold start of the engine. Time-resolved HC and NOx emissions were also
measured at an exhaust port to examine their formation mechanisms and behaviors with fast response HC/NOx analyzers. In conclusion,
the fast burning of fuel and improved combustion quality by enhanced charge motions reduced unburned HC emissions, and advancing
the intake valve opening reduced HC as well as NOx. Furthermore, HCs during the cold transient phase and idle conditions decreased
with recalibrated start parameters such as lean air-fuel ratio and spark retardation via the enhancement of intake charge
motions. 相似文献
226.
G. S. Jung Y. H. Sung B. C. Choi M. T. Lim 《International Journal of Automotive Technology》2009,10(1):1-7
Compression ignition of homogeneous charges in internal combustion (IC) engines is expected to offer high efficiency of DI
diesel engines without high levels of NOx and particulate emissions. This study is intended to find ways of extending the
rich limit of HCCI operation, one of the problems yet to be overcome. Exhaust emissions characteristics are also explored
through analyses of the combustion products. DME fuel, either mixed with air before induction or directly injected into the
combustion chamber of a rapid compression and expansion machine, is compressed to ignite under various conditions of compression
ratio, equivalence ratio, and injection timing. The characteristics of the resulting combustion and exhaust emissions are
discussed in terms of the rate of heat release computed from the measured pressure, and the concentrations of THC, CO, and
NOx are measured by FT-IR and CLD. The experimental data to date show that operation without knock is possible with mixtures
of higher equivalence ratio when DME is directly injected rather than when it is inducted in the form of a perfectly homogeneous
fuel-air mixture. Although fuel injected early in the compression stroke promotes homogeneity of the DME-air mixture in the
cylinder, it causes the mixture to ignite too early to secure good thermal efficiency and knock-free operation at high loads.
Low temperature reactions occur at about 660K regardless of the fueling methods, fuel injection timing and equivalence ratio.
The main components of hydrocarbon emissions turned out to be unburned fuel (DME), formaldehyde and methane. 相似文献
227.
Matthew G. Karlaftis Eleni I. Vlahogianni 《Transportation Research Part C: Emerging Technologies》2009,17(4):444-453
In transportation analyses, autoregressive integrated moving average (ARIMA) and generalized autoregressive conditional heteroskedasticity (GARCH) models have been widely used mainly because of their well established theoretical foundation and ease of application. However, they lack the ability to capture long memory properties and do not jointly treat the mean and variance (variability) of a time-series. We employ fractionally integrated dual memory models and compare results to classical time-series models in a traffic engineering context. Results indicate that dual memory models offer better representation of the original time-series than classical models; further, forcing the differentiation parameter of ARIMA model to equal 1 leads to over-inflated moving average terms and, consequently, to questionable models with artificial correlation structures. 相似文献
228.
Caspar G. Chorus Harry J.P. Timmermans 《Transportation Research Part A: Policy and Practice》2009,43(5):536-547
This paper derives and illustrates measures for the ex ante evaluation of user benefits associated with improvements in the transport system. In contrast with conventional analyses, we assume that awareness among travelers of changes in the transport system is limited and grows over time. Specifically, we postulate that each day (trip) provides travelers with an opportunity to learn about changes that occurred recently in the transport system. This learning process may involve learning through direct experience and indirect learning through for example social networks or information provision. Our measure of user benefits incorporates the conventional logsum-measure as a special case (when full awareness is assumed) and has a closed form solution under reasonable error term assumptions. A numerical illustration provides a first sign of face validity. We derive and discuss a number of practical implications, and discuss possible model extensions. 相似文献
229.
The key features of the western Galician shelf hydrography and dynamics are analyzed on a solid statistical and experimental basis. The results allowed us to gather together information dispersed in previous oceanographic works of the region. Empirical orthogonal functions analysis and a canonical correlation analysis were applied to a high-resolution dataset collected from 47 surveys done on a weekly frequency from May 2001 to May 2002. The main results of these analyses are summarized bellow. Salinity, temperature and the meridional component of the residual current are correlated with the relevant local forcings (the meridional coastal wind component and the continental run-off) and with a remote forcing (the meridional temperature gradient at latitude 37°N). About 80% of the salinity and temperature total variability over the shelf, and 37% of the residual meridional current total variability are explained by two EOFs for each variable. Up to 22% of the temperature total variability and 14% of the residual meridional current total variability is devoted to the set up of cross-shore gradients of the thermohaline properties caused by the wind-induced Ekman transport. Up to 11% and 10%, respectively, is related to the variability of the meridional temperature gradient at the Western Iberian Winter Front. About 30% of the temperature total variability can be explained by the development and erosion of the seasonal thermocline and by the seasonal variability of the thermohaline properties of the central waters. This thermocline presented unexpected low salinity values due to the trapping during spring and summer of the high continental inputs from the River Miño recorded in 2001. The low salinity plumes can be traced on the Galician shelf during almost all the annual cycle; they tend to be extended throughout the entire water column under downwelling conditions and concentrate in the surface layer when upwelling favourable winds blow. Our evidences point to the meridional temperature gradient acting as an important controlling factor of the central waters thermohaline properties and in the development and decay of the Iberian Poleward Current. 相似文献
230.
D. B. Agusdinata J. W. G. M. van der Pas W. E. Walker V. A. W. J. Marchau 《先进运输杂志》2009,43(4):413-454
Road safety is a policy priority due to the high casualties and costs associated with road accidents. Since speed is a major cause of road accidents, in‐vehicle speed limiters or Intelligent Speed Adaptation (ISA), seems a promising solution. ISA implementation, however, is hindered by large uncertainties, for example about the impacts of ISA, the way users might respond to ISA, and the relationship between speed and accidents. Traditional Multi‐Criteria Analysis (MCA) has limitations in handling these uncertainties. We present an MCA approach based on exploratory modeling, which uses computational experiments to explore the multiple outcomes of ISA policies (safety, emissions, throughput, and cost) across a range of future demand scenarios, functional relationships for performance criteria, and user responses to ISA. As an illustration, by testing the impacts of different ISA penetration levels on two driver groups, we show that when compliance with ISA is expected to be low, a policy aimed only at novice drivers outperforms other ISA policies on safety improvement. 相似文献