β淀粉样肽1～42单克隆抗体的制备

目的　制备稳定分泌β -淀粉样肽 1～ 4 2 (Aβ1-42 )单克隆抗体的杂交瘤细胞 ,产生高效价抗体。方法　通过基因工程技术 ,将Aβ1-42 基因融合于乙肝核心抗原 (HBcAg)的主要免疫优势区 (MIR) ,制备Aβ和HBcAg的融合蛋白 ,免疫BALB/c小鼠 ,取脾细胞与SP2 / 0细胞融合 ,筛选能稳定分泌Aβ1-42 单克隆抗体的杂交瘤细胞。结果　得到两株能稳定分泌Aβ1-42 单克隆抗体的杂交瘤细胞 ,Aβ1-42 单克隆抗体的Ig亚类为IgG3 。结论　制备得到的Aβ1-42 单克隆抗体特异性强 ,效价高     

DESIGN OF A DIGITAL SIGNATURE SCHEME BASED ON FACTORING AND DISCRETE LOGARITHMS

Objective Focusing on the security problem of authentication and confidentiality in the context of computer networks, a digital signature scheme was proposed based on the public key cryptosystem. Methods Firstly,the course of digital signature based on the public key cryptosystem was given. Then, RSA and ELGamal schemes were described respectively. They were the basis of the proposed scheme. Generalized ELGamal type signature schemes were listed. After comparing with each other, one scheme, whose Signature equation was (m r)x=j s modФ(p) , was adopted in the designing. Results Based on two well-known cryptographic assumptions, the factorization and the discrete logarithms, a digital signature scheme was presented. It must be required that s“ was not equal to p‘q“ in the signing procedure, because attackers could forge the signatures with high probabilities if the discrete logarithms modulo a large prime were solvable. The variable public key “e“ is used instead of the invariable parameter “3“ in Ham‘s signature scheme to enhance the security. One generalized ELGamal type scheme made the proposed scheme escape one multiplicative inverse operation in the signing procedure and one modular exponentiation in the verification procedure.Conclusion The presented scheme obtains the security that Harn‘s scheme was originally claimed. It is secure if the factorization and the discrete logarithms are simultaneously unsolvable.     

THE GENE EXPRESSION OF BDNF IN NORMAL RABBIT RETINA

Objective To investigate the distribution of brain-derived neurotrophic factor(BDNF) protein in the rabbit retina. Methods Immune response material in the retina was observed using BDNF antibody by the method of immunohistochemistry. Results BDNF gene expression was mainly found in the RGCs, also in innernuclei cells and outernuclei cells in rabbit retina. Conclusion RGC is not only the target cell of BDNF, but also express the BDNF protein. BDNF from multi-sources participates in the regulation of RGCs.     

Multi-Band Hybrid Mobile Phone Antenna Based on Electromagnetic Coupling

This research presents a multi-band hybrid mobile phone antenna based on electromagnetic coupling,which can be applied to mobile handheld devices, occupying a small board space of 39.7 mm × 15.6 mm on the system circuit board. By adding resonant and coupled branch instead of multi-feed on the traditional bent antenna,this design provides four wide operating bands of 0.772—0.998 GHz, 1.540—1.600 GHz, 1.680—2.270 GHz and2.300—2.690 GHz with the hybrid feature of planar inverted-F antenna(PIFA), L-shape, U-shape and S-shape structures, which cover nine-band, i.e., GSM850, GSM900, GPS1575, DCS1800, PCS1900, IMT200, LTE2300,LTE2600 and Blue Tooth/Wi-Fi. Ansoft software HFSS is used in this research to make the antenna performance better and the operating principle of the proposed antenna is described in detail. Result of simulation reveals that the maximum gains of these four wide bands are 2.20,-0.99, 4.01 and 4.05 dBi, respectively. Moreover, this research also tests the return loss(S11) of the fabricated antenna with the vector network analyzer and the result is in accordance with the simulation result on the whole. There are four wide resonant frequencies which cover nine-band of wireless wide area network(WWAN), wireless local area network(WLAN) and long term evolution(LTE), when the available bandwidth is better than 6 d Bi return loss.     

深圳市“轨道+物业”开发实践及发展策略

"轨道+物业"开发模式促进了城市土地集约化利用、为轨道交通建设的可持续发展提供资金保障,在轨道交通行业已形成共识。轨道交通沿线土地综合开发是当前轨道交通企业采用的主要融资模式,是中国主要城市迫切需要推行的模式。阐述对"轨道+物业"开发的认识,对深圳市土地作价出资政策、开发模式、上盖物业与轨道交通建设衔接、持有优质物业等核心问题进行详细分析。探讨未来深圳市轨道交通上盖开发面临政策调整风险、土地资源稀缺、保障性住房建设等多种挑战。最后,提出积极走市场化路线参与城市更新、建设安居型可售人才房和保障房、实施走出去战略、"轨道+物业"开发经验输出等发展策略,以实现轨道交通建设可持续发展。     

Research on Spatially Adaptive High-Order Total Variation Model for Weak Fluorescence Image Restoration

Photoacoustic imaging acquires the absorption contrast of biological tissue with ultrasound resolution. It has been broadly investigated in biomedicine for animal and clinical studies. Recently, a micro-electro-mechanical system (MEMS) scanner has been utilized in photoacoustic imaging systems to enhance their performance and extend the realm of applications. The review provides a recap of recent developments in photoacoustic imaging using MEMS scanner, from instrumentation to applications. The topics include the design of MEMS scanner, its use in photoacoustic microscopy, miniature imaging probes, development of dual-modality systems, as well as cutting-edge bio-imaging studies.     

山地城市支路有效间距研究

合理控制支路系统的规划指标对于畅通城市交通微循环、完善道路网系统具有重要作用.山地城市道路非直线系数往往较大,且干路沿线交叉口多采用右进右出的控制方式,导致路网容量、转向比例等指标不能客观地反映出行者到达目的地的便捷程度.针对山地城市支路建设的特点,提出有效间距的概念,构建能够综合反映交叉口转向、路段绕行和小区内部步行时间的道路网连通效率指标,并通过模拟分析得到支路有效间距的最优值.研究结果表明:支路有效间距宜控制在200 m以内,且最优指标值随着步行速度的增加而单调上升;当支路非直线系数增大时,提高交叉口密度有助于保障道路网络的连通效率.     

基于层次分析法的工程招标资格预审方法

基于工程招标资格预审的特点,建立了层次结构评价模型。运用层次分析法对多位专家的评审意见进行综合分析,从而实现对资格预审这种多方案、多准则的问题进行分析求解。     

STUDY ON THE THERAPEUTIC EFFECTS OF GINSENOSIDE Rg-1 AND GASTRODINE ON AD MODEL RATS INDUCED BY β-AMYLOID PEPTIDE （25-35）

Alzheimer sdisease(AD)isadegenerativedis orderofcentralnervoussysteminhumanbeing.It ischaracterizedbylossofmemory,cognitivede clineandmisbehavior,aswellasoxidativedamage isincreasedandmetabolicrateisdecreased.Major pathologicalmarksofADareextracellarsenil…     

β-淀粉样蛋白增加基底前脑神经元对谷氨酸的易感性

目的探讨β-淀粉样蛋白25-35(Aβ25-35)与谷氨酸(Glu)联合应用,对原代培养的大鼠基底前脑神经元的作用。方法原代培养大鼠基底前脑神经元,应用形态学、MTT法结合ChAT免疫组织化学染色,分别观察Aβ25-35与Glu联合应用,对原代培养的基底前脑神经元存活率、形态学及ChAT免疫反应阳性神经元的影响。结果将100 nmol/L、1μmol/L Aβ分别和20μmol/L Glu联合用于培养的基底前脑神经元,在倒置显微镜下观察,细胞表面粗糙,立体感减弱,胞浆中出现深色颗粒,细胞肿胀或收缩;尼氏染色显示尼氏体减少;MTT检测显示神经元存活率明显下降,100nmol/L Aβ+20μmol/L Glu组与100 nmol/L Aβ组间,1μmol/L Aβ+20μmol/L Glu组与1μmol/L Aβ组间比较均具有统计学差异(P<0.05);ChAT免疫阳性神经元的灰度和截面积均减小。结论Aβ25-35可增加神经元对Glu神经毒性作用的敏感性,表明Glu在阿尔茨海默病发病中起着非常关键的作用。