单纯疱疹病毒2型gG-2"独特区"基因的原核表达及其血清学诊断价值

目的在大肠杆菌中表达HSV-2的gG-2"独特区"基因(gG-2T)并对其免疫学特性进行初步研究。方法克隆HSV-2的gG-2T,并在大肠杆菌中表达gG-2T融合蛋白。通过Western blot和间接ELISA鉴定融合蛋白的活性。结果通过原核系统表达的gG-2T融合蛋白可以与GST多克隆抗体结合并在46 ku附近出现特异性结合条带。并且能够与确诊的HSV-2患者血清反应,而不与HSV-1患者血清和正常人血清反应。结论获得了HSV-2 gG-2T融合蛋白,它可以与GST多克隆抗体特异性结合并且可以同HSV-2型患者血清特异性反应;获得的gG-2T蛋白为研究以gG-2蛋白作为靶区域的诊断试剂盒和疫苗研发打下实验基础。     

基于语料库数据驱动学习模式的外语教学

数据驱动学习(DDL)是一种基于语料库的发现式学习方法,是语言学习者自身在对大量真实语料观察和分析的基础上将问题解决的自主式学习,它是语料库在外语教学与学习中运用的产物,开辟了英语教学的新视野。本文浅析了语料库DLL在外语教学中的具体运用,希望能引起更多学者的探讨,丰富和改善外语教学的内容和质量。     

血管活性肠肽对暂时性脑缺血大鼠梗死体积的影响

目的通过活体实验研究血管活性肠肽(VIP)对脑缺血损伤的神经保护作用。方法大鼠侧脑室内注射VIP后,采用大脑中动脉线栓(MCAO)法诱导建立暂时性局部脑缺血模型。通过神经学的评价对MCAO大鼠进行判定和筛选;TTC染色测定大鼠脑梗死体积;双抗夹心ELISA检测血清S100β的含量。结果MCAO后2 h大鼠呈现不同程度的神经功能损伤症状。评定为1-2级的仅有2例出现明显脑梗死区,3级的为全部。VIP注射后大鼠脑梗死体积明显缩小,较对照组减少约25%(P<0.05);血清S100β浓度也明显降低(P<0.05)。结论以神经学评分3级作为MCAO模型成功的判定标准是较为可靠的。VIP脑内注射可明显减小脑缺血后梗死体积,具有神经保护作用。VIP可以降低S100β的含量,这可能涉及到VIP的另一神经保护机制。     

电子节气门参数自整定模糊PID控制器仿真研究

分析了以功耗低、力矩大、成本较低的普通直流电机作为驱动电机的电子节气门控制系统结构与工作原理,然后设计了节气门参数自整定模糊PID控制器,在一定程度上解决了直流电机作为节气门驱动电机控制不够精确的问题,并通过仿真证明了其用在电子节气门控制中的可行性。     

结合VR技术的汽车制动方向稳定性探讨

在受到侧向力的情况下,进行了整车制动过程的建模和仿真。仿真结果表明:ABS系统以滑移率为控制对象,可提高汽车制动时的方向稳定性,对整车制动的安全性有重要作用。通过VR技术可使仿真结果直观的表现。     

实施物流目标成本事前管理的方法探讨

物流目标成本事前管理是物流企业施行物流目标成本管理的最重要环节,然而在实施过程中却存在着各种问题。本文将站在另外一个角度,对物流企业如何有效实施目标成本事前管理进行尝试性的探索。     

Geometrical design of blade＇s surface and boundary control of Navier-Stokes equations

In this article a new principle of geometric design for blade＇s surface of an impeller is provided. This is an optimal control problem for the boundary geometric shape of flow and the control variable is the surface of the blade. We give a minimal functional depending on the geometry of the blade＇s surface and such that the flow＇s loss achieves minimum. The existence of the solution of the optimal control problem is proved and the Euler-Lagrange equations for the surface of the blade are derived. In addition, under a new curvilinear coordinate system, the flow domain between the two blades becomes a fixed hexahedron, and the surface as a mapping from a bounded domain in R2 into R3 , is explicitly appearing in the objective functional. The Navier-Stokes equations, which include the mapping in their coefficients, can be computed by using operator splitting algorithm. Furthermore, derivatives of the solution of Navier- Stokes equations with respect to the mapping satisfy linearized Navier-Stokes equations which can be solved by using operator splitting algorithms too. Hence, a conjugate gradient method can be used to solve the optimal control problem.     

Study of the mechanism on the apoptosis induced in Human leukemia cell line K562 by the combination of indole-3-acetic acid and horseradish peroxidase

Objective To investigate the mechanisms of apoptosis induced in Human leukemia cell line K562 by the combination of indole-3-acetic acid and horseradish peroxidase. Methods Human leukemia cell line K562 were exposed to indole-3-acetic acid （IAA） at 20, 40, 60, 80 or 100 mol/L and horseradish peroxidase（HRP） at 1.2 g/mL for varying times. MTT assay was applied to detect the cell proliferation. Flow cytometry was performed to detect the arrest of cell cycle. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling （TUNEL） assay was used to measure apoptosis. 2, 7-dichlorofluorescin diacetate （DCFH-DA） uptake was measured to determine free radical by confocal microscope. Content of malondiadehyde （MDA） and activity of superoxide dismutase （SOD） were measured by biochemical methods. Results IAA/HRP initiated growth inhibition of K562 cells in a dose- and time-dependent manner. Flow cytometry revealed that cell cycle arrested at G1/G0 after 24 hours treatment. After 72 hours treatment, apoptotic rate of 100 mol/L IAA group increased to 43.9 %, which was 5 times that of control（P〈0.01）. Content of MDA and activity of SOD increased respectively in treatments compared with control. Meanwhile, IAA/HRP stimulated the formation of free radical, which was increased by IAA concentration-dependently. Conclusion The combination of IAAand HRP can inhibit the growth of Human leukemia cell line K562 in vitro by inducing apoptosis which is associated with the increase of free radical. The combination of IAA and HRP might be a promising chemopreventive and chemotherapeutic agent against human leukemia.     

乌龙江大桥无覆盖层河床的钻孔平台设计

介绍在水深流急、潮位变化大、无覆盖层的深水中的基础施工平台设计,平台的稳定、精确定位、安全渡汛抗台是平台设计和施工的关键因素。     

基于单片机和Fuzzy技术的液压在线故障监测系统的设计

介绍了基于单片机和Fuzzy技术的液压在线故障监测诊断系统的设计,给出了系统组成和软件设计。该系统以高性能单片机为核心,采用Fuzzy逻辑分析技术实现了液压系统故障诊断的基本结构和知识库的方法,建立了液压系统故障Fuzzy分析的逻辑结构,实验表明,该系统提高了液压系统故障推理的正确性和推理速度。