The β-amyloid protein induces S100β expression in rat hippocampus through a mechanism that involves IL-1

Objective To explore the effect of β-amyloid protein (Aβ) on S100β expression in rat hippocampus and its mechanisms. Methods At 7 days after bilateral stereotaxis injection of different dose of fibrillar Aβ 25-35 and interluekin-1 receptor antagonist (IL-1ra) into the rat CA1 region, the learning and memory abilities of rats were tested with passive avoidance task. Amyloid deposition was detected by using Congo red staining technique. Nissl staining and immunohistochemical techniques were used to analyze the number of neurons, and GFAP and the S100β expression in hippocampal CA1 region , respectively. Results After fibrillar Aβ injection, the step-through latency of rats was significantly shortened compared to that of the control group. The GFAP positive astrocytes were found surrounding amyloid deposition. Neuronal loss occurred in the pyramidal cell layer of CA1 region. The number of S100β positive cells in Aβ-treated group was significantly increased compared with that in the control group. After IL-1ra injection, the number of S100β positive cells was significantly decreased. Conclusion Intrahippocampal injection of Aβ 25-35 could cause similar pathologic changes of Alzheimer's disease. Aβ 25-35 was capable of up-regulating S100β expression in a dose-dependent manner. The injection of IL-1ra could attenuate the effect of Aβ on S100β expression.     

PROLIFERATION AND MIGRATION OF EPENDYMAL CELLS IN THE BRAIN OF ADULT RATS AFTER PERMANENT FOCAL CEREBRAL ISCHEMIA

Objective Ependymal cells are thought to be the primary source of neural stem cells in the adult central nervous system. The purpose of this study is to examine spatial and temporal profiles of ependymal cell proliferation and migration after focal cerebral ischemia. Methods Eighty male Sprague Dawley rats underwent permanent middle cerebral artery occlusion after injection of 10/μL of 0.2% Dil into the lateral ventricle. Rats were sacrificed and brain sections were acquired for pathological evaluation and laser confocal imaging at day 1,3,7,11,14,21 and 28 after ischemia. Results The density of Dil-labeled cells in the ischemic ipsilateral subventricular zone was significantly higher than that in the control group and these labeled cells dispersed in the ischemic ipsilateral subventricular zone and/or were located in ependyma from day 1 to 11. In the ischemic ipsilateral cortex, some Diilabeled cells occurred in peri-infarction and infarction of parietal region at day14 and peaked at day 21 when some Dil-labeled cell nodules were found in this region. During postischemic day 14--28, a significant decrease in labeled celldensity in the ischemic ipsilateral subventricular zone was coincident with a significant increase in labeled cells density in the cortex (peri-infarction and infarction). Conclusion The results indicate that ependymal cells proliferate and migrate after focal cerebral ischemia in the adult rat brain.     

LM331在内燃机车检测中的应用

本文给出一种应用于内燃机车水阻测试台的主电压检测电路,该电路使用廉价的单片V/F转换器,通过光电耦合方式,既保证了检测电压的精度,又可有效防止各种干扰串入计算机控制系统。     

PROPERTIES OF PROLIFERATION AND DIFFERENTIATION OF NEONATAL RAT RETINAL PROGENITOR CELLS IN VITRO

Objective To investigate the properties of proliferation and differentiation of neonatal rat retinal progenitor cells （RPCs） in vitro. Methods RPCs were isolated from neonatal SD rats neural retina and cultured in DMEM/F12 ＋ N2 with EGF and bFGF （suspension medium ） or 10 % FBS without EGF and bFGF （ differentiation medium）. The cells grew as suspended spheres or adherent monolayers, depending on different culture conditions. The neural stem cells or retinal progenitors, neurons, astrocytes, retinal ganglion cells, rod photoreceptors and the proliferating cells were evaluated with immunofluorescence analysis by Nestin or Pax6, Map2, GFAP, Thy-1, Rhodopsin and BrdU antibodies respectively. Results RPCs could propagate and differentiate in suspension or differentiation medium and express the markers of Nestin （92.86%） or Pax6 （86.75%）, Map2 （38.54%）, GFAP （20.93%）, Thy-1 （27. 66%） and Rhodopsin（13. 33%）in suspension medium; however, Nestin （60.27%）, Pax6 （52%）, Map2 （34.94%）, GFAP （38.17%）, Thy-1 （30.84%） and Rhodopsin （34.67%） in differentiation medium. 96.4 % of the population in the neurospheres was BrdU-positive cells. The cells could spontaneously adherent forming some subspheres and retinal specific cell types. Conclusion Neonatal rat RPCs possess the high degree of proliferation and can differentiate into neurons, astrocytes, retinal ganglion cells and rod photoreceptors in vitro. There are different proportions for RPCs to differentiate into specific cell types.     

结构多位置损伤的线性诊断方法

引入了一阶振型变化率的概念，并且证明了一阶振型变化率具有可叠加性，多位置损伤所引起的一阶振型变化率是各单位置损伤引起的一阶振型变化率的线性叠加．以一阶振型变化率为损伤标识量，将结构的多位置损伤状态等价于各单位置损伤状态的叠加．列出了结构多位置损伤的线性损伤诊断方法的实现步骤．以简支梁和四边固支的正方形板的多位置损伤诊断为算例表明，该方法不仅可准确地判定结构的损伤状态，而且损伤诊断的过程简单．     

汽油车排放法规和控制技术的发展研究

简要回顾了汽车排放法规的发展过程,对于发展的各个阶段,分析了具有针对性的汽车排放污染控制技术的内容和特点。研究了随着汽车排放法规和汽车工业的发展,汽车排放污染控制的前景及汽车排放控制技术的发展方向。     

申嘉湖京杭古运河大桥设计浅议

针对申嘉湖高速公路京杭古运河大桥提出了预应力混凝土连续梁桥和斜拉桥两种不同的桥式方案。通过经济性、安全性、实用性、耐久性和施工等方面的比选,建议采用55m 80m 55m的变截面连续箱梁桥方案,并运用桥梁博士对主梁进行了施工阶段和使用阶段的内力计算和估索计算。验算结果表明,所选的预应力混凝土连续梁桥方案是合理的。     

基于时间Petri网的集装箱码头AGV调度系统建模研究

在分析了集装箱自动化码头物流系统中的自动导引小车-AGV工作情况的基础上，提出了利用时间Petri网对AGV调度系统建模和性能分析的基本思路；建立了AGV的运输路径模型，并进行了系统性能特性分析；结果表明这种模型能够准确地描述集装箱码头物流系统的运行状况．     

中国城市道路交通安全特点解析

由于中国正处于机动化、城镇化快速交织发展时期,城市道路与公路交通安全的发展特点不同。针对城市道路交通安全特点,基于2007—2016年城市道路交通事故和交通违法数据,兼顾时序规律和空间规律,梳理归纳城市道路交通安全发展的阶段性特征。进而解析城市道路交通安全特点的形成原因,探讨道路安全与道路设施、出行方式、出行行为之间的内在联系。最后,针对城市道路交通事故发生时间、交叉口和非机动车道交通事故回升、电动自行车交通事故骤增、货车夜间交通事故多发、行人交通事故伤亡数居高、老年群体交通安全风险升高等特征,分别提出推进交通安全管理的对策建议。     

An Efficient Forward Secure Signature Scheme

Introduction Exposureofsecretkeysthreatensthesecurity ofadigitalsignaturegreatly.Totacklethisprob-lem,severaldifferentmethodshavebeenpro-posed,includingsecretsharing[1],thresholdsigna-ture[2],andproactivesignature[3].Thesemethods,however,needcooperativeandinteractivecompu-tationsinmultiplesevers,whicharequitecostly.Forwardsecuresignatureschemecanreducethe damageofkeyexposurewithoutcooperativeand interactivecomputations.Intheparadigmoffor-wardsecuresignature,thewholelifetimeofsigna-tureisdivid…