苦参碱对人卵巢癌细胞增殖的影响及其机制

目的 研究苦参碱(matrine)体外培养的人卵巢癌细胞OV2008增殖的影响及相关机制.方法 采用四甲基偶氮唑蓝(MTT)比色法检测不同浓度(0、0.125、0.25、0.5、1.0,2.0、4.0 mg/mL)的苦参碱在不同的时间点(24、48、72 h)对OV2008细胞增殖的影响;采用流式细胞术(FCM)检测不同浓度的苦参碱作用于OV2008细胞48 h后细胞周期分布、凋亡率及Fas、Bcl-2蛋白的表达水平.结果 苦参碱对OV2008细胞的增殖具有明显的抑制作用,与对照组相比具有显著性差异(P<0.05或P<0.01),并呈现时间和剂量依赖性;细胞周期分析显示,随着苦参碱浓度的增高,G0/G1期细胞比例逐渐增加,S期细胞比例减小,G2/M期细胞比例无明显变化;苦参碱能够诱导细胞凋亡,且凋亡率随着苦参碱浓度的增高而升高(P<0.01);苦参碱能够上调Fas蛋白的表达(P<0.05或P<0.01),下调Bcl-2蛋白的表达(P<0.01).结论 苦参碱可显著抑制OV2008细胞的增殖,且抑制作用晕剂量和时间依赖性.苦参碱对OV2008细胞的增殖抑制作用可能与其诱导细胞周期阻滞及凋亡有关,而苦参碱诱导凋亡可能与其上调Fas蛋白表达、下调Bcl-2蛋白表达有关.     

基于模糊综合评价模型的轨道交通安全性评价

为保障轨道交通的安全运行,需要对轨道交通进行安全性评价．针对影响轨道交通安全的各种因素,采用定性与定量相结合的方法,从运营管理、设施设备、从业人员及外部条件等4个方面,建立了层次结构的评价指标;根据层次分析法,确定了各项评价指标的权重;用模糊数学方法,建立了轨道交通安全性评价的二级模糊综合评价模型;对轨道交通的安全性,进行了综合评价．以对南京市地铁系统为实例进行分析,说明了模糊综合评价模型的适用性和合理性．     

孔结构对水泥混凝土性能的影响研究

介绍水泥混凝土中孔结构的分析测试方法,着重分析孔结构对水泥混凝土的力学性能、低温性能、抗渗性能的影响,探讨孔结构对混凝土的作用机理,提出了改善水泥混凝土孔结构的措施,并对今后的研究重点给出了建议。     

黄土地基的冲击压实研究

结合黄土的特性,选择预建高速公路的典型黄土,通过室内试验方法确定黄土的物理指标。通过黄土击实试验确定了该试样的最佳含水量为14.3%,与之相应的最大干密度为1.918 g/cm3。最后选择冲击式压路机在试压路段进行碾压,通过试验数据可知,在冲击压实达到40遍时,地面层20 cm的黄土的压实度能达到97.8%,黄土地基的总沉降量达到25.12 cm,且沉降趋于稳定。     

Study on launch dynamics of the tank marching fire

As an army main battle equipment, it is required that the tank should have high firing accuracy and high first round hit probability during marching. The initial disturbance of the projectile is the premier factor that takes effect on the marching fire accuracy of the tank. And the marching fire accuracy of the tank depends on the launch dynamics behaviors of the tank. In this paper, the launch dynamics theory of a tank marching fire is studied, and its launch dynamics model is established. Based on the transfer matrix method for multibody system(MSTMM) and the automatic deduction theorem of overall transfer equations, the overall transfer equation and the overall transfer matrix of a tank multibody system are deduced; the launch dynamics equations of the tank marching fire are deduced, and the dynamic response of the tank system, the motion of projectile in barrel, the initial disturbance of the projectile and the vertical target dispersion are exactly simulated; meanwhile, the results of simulation are verified by tests. This work provides both theoretical foundation and simulation approaches for improving the marching fire accuracy of the tank.     

Dynamics of inorganic nutrient species in the Bohai seawaters

Within the frame of a Sino-German Joint Research Program, two cruises of “R/V Dong Fang Hong 2” were carried out in September–October 1998 and April–May 1999, respectively, to understand the dynamics of nutrients in the Bohai. Nutrient species (NO₃⁻, NO₂⁻, NH₄⁺, PO₄³⁻ and SiO₃²⁻) are determined colorimetrically on board for five anchor and 30 grid stations. In situ incubation experiments are performed to determine planktonic nutrient uptake and benthic exchange flux. Nutrient concentrations display short-term variability and seasonal change in the Bohai, with higher levels in shallow coastal waters than in the Central Bohai. The influence of riverine discharge on nutrient levels can be seen from salinity isopleths, nutrient distribution and species ratios. Near-bottom (nb) waters have similar nutrient concentrations as to the surface waters in the Central Bohai, whereas stratification takes place in the Bohai Strait and North Yellow Sea. In situ incubation experiments provide evidence that the uptake ratio (i.e. N, P) by phytoplankton is proportional to the ratios among nutrient species in ambient waters. Based on the data of this study and previously publications, a preliminary estimate of nutrient budgets via riverine input and atmospheric deposition is established. The results indicate that atmospheric deposition gains importance over rivers in delivering nutrients into the Bohai and sustain the new production, following recent decrease in riverine inflow caused by drought periods in North China and damming practices. A historical review of nutrient data indicates that concentrations of nitrogen increase and phosphorus and silica decrease in the Central Bohai over last 40 years. This potentially has an important influence on the health of ecosystem in Bohai (e.g. food web and community structure), though further study is needed to examine the scenario in more detail.     

“十四五”我国港口货物吞吐量结构化预测*

为更好分析港口运输需求发展趋势,将港口吞吐量影响因素进行结构拆解,建立吞吐量与国内生产总值、工业增加值、一二三产业增加值、投资、消费、外贸额的关系方程。同时,将吞吐量拆分为主要货类,建立煤炭吞吐量与火电发电量、原油吞吐量与原油加工量、铁矿石吞吐量与生铁产量、集装箱吞吐量与第二产业增加值等关系方程。组合应用回归分析、弹性系数等预测模型,预测2025年我国港口货物吞吐量、煤炭、原油、铁矿石和集装箱吞吐量分别为172亿、29亿、8.3亿、22亿t和3.2亿TEU。预测结论为全国水运“十四五”发展规划等提供参考。     

Tribon环境下船体分段拼板图自动设计方法研究

船体分段拼板图设计是船舶建造生产设计中的重要内容,目前主要依靠人工绘制,效率较低。基于Tribon的GEN文件对分段拼板图自动设计方法展开了研究,通过提取拼板图设计必需的图形信息并进行自动区域划分和定义,确定各类型数据间的相互逻辑关系。在此基础上,确定各类标注尺寸的自动计算方法,实现分段拼板图的自动设计,不仅能够提高拼板图设计的准确性,而且能够提高拼板图的设计效率。     

沿海港口发展环境及适应性分析

立足于国际经贸环境、中国国民经济以及中国对外贸易发展和产业布局调整的最新形势,分析当前全国沿海港口发展的阶段性特点及面临的宏观环境。提出码头评估能力的概念,客观分析港口吞吐量统计数据的构成,就沿海港口评估能力与运输需求的总体适应性、分区域适应性、发展结构适应性以及与产业布局适应性进行探讨,提出问题及建议,展望港口发展态势。     

大断面水下盾构隧道管片接头抗弯刚度及其对管片内力影响研究

以大断面水下铁路盾构隧道-狮子洋隧道工程为研究对象,运用有限元数值分析方法,并结合管片接头原型抗弯试验,研究环向管片接头抗弯刚度,并运用梁-弹簧模型进行接头抗弯刚度对整环管片结构内力影响的研究.结果表明:该隧道管片接头抗弯刚度的取值范围为50～700MN·m·rad-1,在相同轴力条件下,接头抗弯刚度会随接头弯矩的增加降低1个数量级左右;在相同接头弯矩条件下,接头抗弯刚度随轴力的增加而增大;接头抗弯刚度对管片轴力分布的影响微弱,对管片弯矩的影响显著;随接头抗弯刚度的增大,整环管片的弯矩分布趋于均匀;在抗弯刚度取值范围内,极值弯矩相差最大达80％左右,极值轴力最大减小5％左右,变形最大减小20％左右;基于接头抗弯刚度-弯矩-轴力的非线性关系改进的梁-弹簧模型,更能体现接头对整环管片受力的影响,也更适用于大断面盾构隧道管片内力的计算.