电子节气门参数自整定模糊PID控制器仿真研究

分析了以功耗低、力矩大、成本较低的普通直流电机作为驱动电机的电子节气门控制系统结构与工作原理,然后设计了节气门参数自整定模糊PID控制器,在一定程度上解决了直流电机作为节气门驱动电机控制不够精确的问题,并通过仿真证明了其用在电子节气门控制中的可行性。     

轿车乘坐方式探讨

分析研究了轿车内室空间布局,提出轿车内室有2种乘坐方式的设计构想,既能达到普通乘坐方式舒适的要求,又能在乘员需要方便交流的情况下,有一个更合适的乘坐方式。     

钻孔施工设备的新选择——旋挖钻机

本文介绍了钻孔灌注桩施工中的一种新设备。     

基于CFD的空气滤清器入口结构改进设计

采用基于时均Navier-Stokes方程和高雷诺数k-ε紊流模型,对某一型号空气滤清器入口部分进行了流动计算,并根据计算结果对压力、速度特性进行了分析,提出了改进模型。通过对改进后模型的计算,可以看出内部流场和流速的分布都得到了改善,证明改进后的结构更符合气体流动特性。     

关于带误差的Ishikawa迭代过程

在一致凸Banach空间中研究了非扩张映射序列的带误差Ishikawa迭代过程的构造和收敛问题,推广了近期的结果。     

基于灰色关联度法的轨道交通线网评价

通过对城市轨道交通线网评价和灰色系统相关理论的介绍，提出了应用于城市轨道交通线网规划的灰色评价模型。该模型通过比较各方案与理想方案之间关联度的大小来区分各方案的优劣，并通过实例验证了这种方法的应用能提高轨道交通线网规划评价的客观性、公正性、合理性。该方法可以为城市轨道交通网络规划方案的评价及方案优选服务，避免因人为失误而造成的决策偏差。     

紫杉醇诱导人肺腺癌多药耐药细胞系的建立及DNA polβ、mdr1、mrp1、GST-πl、rp和topo Ⅱ基因的表达

目的体外建立人肺腺癌细胞多药耐药细胞系,观察其生物学特性并探讨其细胞内DNA polβ和多药耐药基因mdr1、mrp1、GST-πl、rp和topo Ⅱ的表达及意义。方法以紫杉醇为诱导药,采用间歇逐步增加剂量法建立人肺腺癌多药耐药细胞系A549/TXL20;MTT法检测A549/TXL20对紫杉醇、顺铂、长春新碱、5-氟尿嘧啶和丝裂霉素的耐药指数(RF);细胞克隆形成法测定A549/TXL20对紫杉醇的敏感性;高效液相色谱测定细胞内紫杉醇的浓度;RT-PCR法测定人肺腺癌多药耐药细胞系A549/TXL20中DNA polβ、mdr1、GST-π、mrp1l、rp和topo Ⅱ的基因表达。结果①A549/TXL20形态不规则,较亲本细胞略小,核/浆比增加,倍增时间没有明显变化;②A549/TXL20对紫杉醇的RF为19.3,对顺铂的RF为67.4,对长春新碱、5-氟尿嘧啶、丝裂霉素和顺铂等抗癌药物有不同程度的耐药性;③A549/TXL20对紫杉醇的敏感性降低,其紫杉醇的含量较亲本细胞下降;④A549/TXL20细胞中DNA polβ、mdr1、GST-π、mrp1l、rp的基因表达量分别较A549细胞中的表达量明显增加,差异具有统计学意义(P<0.05)。结论建立了相对稳定的人肺腺癌多药耐药细胞系A549/TXL20,其耐药机制可能与mdr1、GST-π、mrp1l、rp和DNA polβ基因的高表达有关。     

Efficient iris recognition via ICA feature and SVM classifier

With the increasing demands of security in ournet worked society,the technology for personalidentification works as the main solution to safe-guard people s properties.Biometrics is an alterna-tive to solve the problemand has the advantage thatthey cannot be stolen or forgotten like pass words.Because personal identification numbers or identifi-cation tokens(such as s mart cards)cannot provide ahighlevel of security which can be copied,inspec-ted and/or stolen.They only showthe knowledgeof som…     

Calculation of VPP basing on functional analyzing method

VPP can be used to deter mine the maxi mumvelocities of a sailboard at various sailing-routes,byestablishing the forces and moments balance-equa-tions on the sail and board in accordance with theprinciple of the maxi mal drive-force.Selectingroute is the most i mportant issue in upwind-sailing,and VPP calculations could provide the basis for de-ter mining the opti mal routes.VPP calculation of the sailboard perfor mance isa complex and difficult research task,and there arefew projects in thi…     

Study of the mechanism on the apoptosis induced in Human leukemia cell line K562 by the combination of indole-3-acetic acid and horseradish peroxidase

Objective To investigate the mechanisms of apoptosis induced in Human leukemia cell line K562 by the combination of indole-3-acetic acid and horseradish peroxidase. Methods Human leukemia cell line K562 were exposed to indole-3-acetic acid （IAA） at 20, 40, 60, 80 or 100 mol/L and horseradish peroxidase（HRP） at 1.2 g/mL for varying times. MTT assay was applied to detect the cell proliferation. Flow cytometry was performed to detect the arrest of cell cycle. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling （TUNEL） assay was used to measure apoptosis. 2, 7-dichlorofluorescin diacetate （DCFH-DA） uptake was measured to determine free radical by confocal microscope. Content of malondiadehyde （MDA） and activity of superoxide dismutase （SOD） were measured by biochemical methods. Results IAA/HRP initiated growth inhibition of K562 cells in a dose- and time-dependent manner. Flow cytometry revealed that cell cycle arrested at G1/G0 after 24 hours treatment. After 72 hours treatment, apoptotic rate of 100 mol/L IAA group increased to 43.9 %, which was 5 times that of control（P〈0.01）. Content of MDA and activity of SOD increased respectively in treatments compared with control. Meanwhile, IAA/HRP stimulated the formation of free radical, which was increased by IAA concentration-dependently. Conclusion The combination of IAAand HRP can inhibit the growth of Human leukemia cell line K562 in vitro by inducing apoptosis which is associated with the increase of free radical. The combination of IAA and HRP might be a promising chemopreventive and chemotherapeutic agent against human leukemia.