TSH RECEPTOR GENETIC ALTERATIONS IN THE AUTONOMOUSLYFUNCTIONING THYROID ADENOMAS

Objective To determine the relationship between TSH receptor gene mutations and autonomously functioning thyroid adenomas (AY‘]rAs). Methods The thyroid samples from 14 cases of diagnosed AFTAs were analyzed, with normal thyroid specimens adjacent to the tumors as controls. The 155 base pairs DNA fragments which encompassed the third cytoplasmic loop and the sixth transmembrane segments in the TSH receptor gene exon 10 were amplified by Polymerase chain reaction (PCR) and analyzed by the single-strand conformation polymorphism (SSCP). Direct sequencing of the PCR products was performed with Prism Dye Terminator Cycle Sequencing Core Kit.Results 6 of 14 AFTA specimens displayed abnormal migration in SSCP analysis. In sequence analysis of 3 abnormally migrated samples, one base substitution at nucleotide 1957 (A to C) and two same insertion mutations of one adenosine nucleotide between nucleotide 1972 and 1973 were identified. No mutations were found in controls. Conclusion This study confirmed the presence of TSH receptor gene mutations in AFTAs; both one-point substitution mutation and onebase insertion mutation were found to be responsible for the pathogenesis of AFTAs.     

电子商务综合发运跟踪系统的研究

基于中国电子商务和物流业的发展现状，提出了采用3层开发模型实现综合发运跟踪服务的设计方案，详细讨论了数据库表设计、应用系统间信息的表示和处理、基于2层隧道协议（Layer 2 Tunneling Protocol,L2TP)的拨号虚拟专用网（Virtual Private Network,VPN)技术的网络结构设计和查询反馈关键技术的实现问题。     

The Size Effects on Process Design of Micro Deep Drawing

This paper suggested to reformulate cylindrical deep drawing parameters with dimensionless form. A diagram, in which a feasible zone is drawn to bound both the maximal allowable tension and compression stress during the deep drawing process, was established. Since it is presented in a dimensionless form, it may be applied for both conventional and micro deep drawing. Cylindrical cup deep drawing was taken as an example to show the dimensionless process design method. In addition, the size effects should be taken into account. Two kinds of size effects on micro deep drawing were investigated, which can be explained by surface layer model and strain gradient model. Numerical simulations were carried out to compare the strain distribution with or without consideration of size effect.     

THE EXPERIMENTAL STUDY ON THE CELL APOPTOSIS AND EXPRESSION OF BCL-2 PROTEIN IN INTRACEREBRAL HEMORRHAGE IN MODEL OF RATS

Intra-cerebral hemorrhage is a common clinicaldisease,with a high mortality and morbidity.So itis one of the clinical hot topics.It has been foundinrecent years that there is a close relationship bet weenthe cell apoptosis and the course or prognosis of in-tra-cerebral hemorrhage.Bcl-2,as the apoptosis-adjusted gene,plays ani mportant role in the courseof cell apoptosis,but the mechanis min the cell ap-optosis in intra-cerebral hemorrhage remains un-clear.In this experi ment,with the model bui…     

关于提高公路工程建筑材料调查的准确性和可行性的初探

对提高公路工程建筑材料调查的准确性和可行性进行了探讨     

甲状腺癌组织端粒酶逆转录酶和P53基因表达及其临床意义

目的　探讨端粒酶逆转录酶 (hTERT)和P5 3基因与甲状腺癌的发生、发展及预后的关系以及它们的相互关系。方法　应用免疫组织化学SP法检测 6 0例甲状腺癌、10例正常甲状腺组织、10例结节性甲状腺肿及 10例甲状腺腺瘤组织中hTERT和P5 3的表达。结果　hTERT和P5 3阳性率在甲状腺癌中增高 (P <0 .0 5 ) ;hTERT与P5 3阳性率在未分化癌、有淋巴结转移或Ⅲ、Ⅳ期病例中增高 (P <0 .0 5 ) ;hTERT表达与P5 3表达具有相关性 (P <0 .0 5 )。结论　hTERT与P5 3的过度表达在甲状腺癌的发生发展过程中具有重要作用 ,P5 3突变在转录水平上调控hTERT基因的表达 ,激活端粒酶可能是甲状腺癌发生发展的重要阶段 ;hTERT和P5 3在甲状腺癌中表达的变化可用于评估其生物学行为和判断预后。     

灵长类主要运动区和背侧运动前区的丘脑联系

目的　观察灵长类主要运动区和背侧运动前区的丘脑起源。方法　采用束路示踪技术 ,将 2种逆行示踪剂分别注射到猴子的主要运动区 (M 1)和背侧运动前区 (PMd)。结果　丘脑腹外核嘴侧部 (VLo) ,腹内侧核(VM ) ,腹后外侧核嘴侧部 (VPLo) ,腹外侧核尾侧部 (VLc) ,中央中核 (CM ) ,中央外侧核 (CL)和腹后内侧核(VPM )均有纤维投射到M 1。丘脑腹外侧核嘴侧部 (VLo) ,腹后外侧核的嘴侧部 (VPLo) ,腹外侧核的尾侧部(VLc)和背内侧核 (MD)则主要投射到背侧运动前区 (PMd)。结论　M1和PMd具有不同的丘脑起源 ,这种起源既有重叠也有分离 ;小脑和基底神经节通过不同的丘脑核团影响运动区皮层的活动     

浅谈资质证书编号的设计

本文就资质管理中资质证书的编号如何更加实用合理进行了探讨.     

门座起重机四连杆组合臂架结构的动力学分析及仿真

结合门座起重机四连杆组合臂架结构在货物起升及变幅运动过程中的柔度影响，采用ADAMS和ANSYS软件相结合的方法将臂架作为柔性体构建动力学模型，进行动力学分析计算与仿真，为臂架系统设计提供了一种实用的动力学分析手段。     

一种基于广度优先遍历的网络拓扑发现算法及其自适应研究

提出的网络拓扑发现算法，综合了传统网络拓扑发现算法的长处．该算法以SNMP协议和TCP／IP网络管理体系为依托，以图的广度优先遍历算法为基础，将各种可以利用的协议和发现技术有机地结合起来，能够准确有效地自动发现并获取网络拓扑信息，克服单一基于SNMP的路由表的拓扑发现算法的不足，并具有很好的自适应性．