海运服务贸易绿色壁垒及对策

通过对海运服务贸易“绿色壁垒”的研究和分析，从政府和航运企业两个角度提出积极的应对策略。     

我国港口企业要加快“走出去”的步伐

“走出去”和“引进来”都是利用国际资源更高级的形式。港口是我国国内(不包括港、澳、台,下同)交通部门中,最早引进外资的领域。在引进来方面,十几年来,中外合资的港口已遍布全国,新建的集装箱码头除洋山一期外,几乎都有外资的介入,这些对我国港口的发展起了巨大的推动作用,保证了我国在加入WTO 后对外贸易的飞速增长,使港口成为开辟国内外两个市场,利用国内外两种资源的有力工具。但在“走出去”上,我国虽已经启动了走向国际港口市场的步伐,两个大型航运企业已经在国外投资建设了自己的码头。但与我国国家实力,我国港口企业的实力和我国港口在     

AIS系统与船舶保安

针对AIS系统的特点和所传输的信息，分析了AIS在远洋船舶上全面安装及实施后，在增加航行安全，提高船舶交通管理系统的服务和监控能力的同时，在船舶保安方面可能出现的问题。建议船舶在使用AIS数据信息时应当采取合理的谨慎态度，尤其是在海盗多发地带应适时地暂时关闭系统。     

热水锅炉供热系统的技术改造及应用

冬季采暖热水锅炉，效率不高和尾部受热面腐蚀现象比较严重，针对15 t的XL900-13/95/70-AⅢ型热水锅炉进行了分析，并制定了相应的节能技术改造措施，在实际应用中取得了良好的效果。     

客运专线车站接触网平面布置中的景观设计

概述了客运专线车站接触网平面布置景观设计的重要性,同时考虑其功能、美学、经济方面的要求,提出了增强美观性的措施:采用H型钢柱,接触网支柱的高度尽量一致,接触网支柱平面布置采用合理的形式。在满足安全性与可靠性的前提下,力求接触网与周围的环境协调,达到与车站整体景观设计融合为一体的目的。     

Preparation of recombinant firefly luciferase by a simple and rapid expression and purification method and its application in bacterial detection

A simple and rapid expression and purification method of recombinant firefly luciferase was developed for bacteria detection. A modified luciferase gene from North American firefly Photinus pyralis was cloned into pET28a expression vector and the recombinant protein was produced in Escherichia coli BL21. The recombinant luciferase, equipped with a polyhistidine affinity tag, was purified by immobilized metal ion affinity chromatography (IMAC). The approach generated an abundant expression and an efficient purification of a recombinant luciferase with final yield 1.995mg/L of cell culture. Experiments on the recombinant luciferase also showed that the relative light units (RUL) of the enzyme were 5.8×108, and the specific activity was 2.9×1010 RLU/mg. By applying adenosine triphosphate (ATP) bioluminescence to detection of the coin bacteria using the recombinant protein, the ATP content of bacteria was 9.48×10-16mol/mL, and was identical to the bacteria counts (4500CFU/mL) in order of magnitude. Taken together, our results provided a simple and efficacious method of the preparation of recombinant luciferase, which could be applied in the determination of bacteria via ATP bioluminescence.     

西安地区0—7岁儿童身体发育五项指数特征的分析

本文应用西安地区1985年测定的17600名0～7岁健康儿童的发育资料,按性别、年龄、城郊分组,统计分析了这一年龄阶段五项常用的发育指数。结果表明各项指数的变化规律和目前国内外报道的一致,且男女之间、城郊之间存在着一定的差异。本文亦对应用指数评价儿童生长发育状况进行了讨论。     

防碰头车门装置的设计

一般的轿车车门都较低,出入车很容易碰头,针对这一普遍存在的问题,在车门门框处增加缓冲板随车门联动机构,以防止乘客进出时碰头。     

轮胎驻波现象与胎压的正确选择

造成汽车轮胎爆胎的原因很多,但引起爆胎的实质原因主要在于轮胎产生的"驻波"现象.轮胎的驻波现象又与轮胎气压的高低有着密切的关系. 1 汽车轮胎的驻波现象众所周知,汽车的全部重量都由轮胎所支承,在重力作用下轮胎胎面与地面的接触部位会有轻微变形,车辆行驶时变形的部分在离开路面后将恢复原状.若选取轮胎胎面与地面接触的一个小段来分析,轮胎每旋转一圈,这一小段就会发生一次变形和恢复的过程,即轮胎发生一次弹性变形.     

Airline emission charges: Effects on airfares,service quality,and aircraft design

This paper explores the effect of airline emissions charges on airfares, airline service quality, aircraft design features, and network structure, using a detailed and realistic theoretical model of competing duopoly airlines. These impacts are derived by analyzing the effects of an increase in the effective price of fuel, which is the path by which emissions charges will alter airline choices. The results show that emission charges will raise fares, reduce flight frequency, increase load factors, and raise aircraft fuel efficiency, while having no effect on aircraft size. Given that these adjustments occur in response to the treatment of an emissions externality that is currently unaddressed, they represent efficient changes that move society closer to a social optimum.