The role of interleukin-8 produced by tumor induced fibroblasts in the development of cutaneous melanoma

Objective To determine the role of interleukin-8 (IL-8) produced by tumor induced fibroblasts in the development of cutaneous melanoma. Methods B16 melanoma cells induced L929 fibroblasts phenotype was transdifferentiated to myofibroblasts (MF) by co-culture in vitro. MF was monitored by morphology and immunophenotype for a-SMA. The level of IL-8 was detected by ELISA. The effect on B16 cell proliferation rate was estimated using MIT method in vitro. Melanoma implanting model was constructed in C57 mice. Results L929 MF phenotype could be modulated by B16 melanoma cells-derived transforming growth factor-β1 (TGF-β1) and elevated the levels of IL-8. L929 MF did not influence the B16 melanoma cells viability in vitro, but shortened the time of tumor formation and increased the incidence rates of tumors in C57 implanting model mice. Conclusion Fibroblasts can be activated by tumor cells and produce IL-8, which acts as an inflammatory cytokine promoting the development of cutaneous melanoma.     

南京长江第四大桥首级控制网的建立

介绍了南京长江四桥首级施工控制网的建立及特点．对如何采用当前测绘新技术布测特大型桥梁首级控制网并提高其成果精度、可靠性等相关技术指标进行了探讨,可供类似精密工程项目参考。     

Wind pressure distribution and wind-induced dynamic response for spatial groined latticed vaults

The wind pressure distribution and wind-induced vibration responses of long-span spatial groined latticed vaults (SGLVs) were numerically simulated, which always are ones of the most important problems in the structural wind resistance design. Incompressible visco-fluid model was introduced, and the standard k-εtwo equation model and semi-implicit method for pressure linked equation (SIMPLE) were used to describe the flow turbulence. Furthermore, the structural dynamic equation was set up, which is solved by Newmark-β method. And several sort of wind-induced vibration coefficients such as the wind-induced vibration coefficient corresponding to the nodal displacement responses and wind loads were suggested. In the numerical simulation where the SGLV consisting of the cylindrical sectors with different curved surface was chosen as the example,the influence on the relative wind pressure distribution and structural wind-induced vibration responses of the closed or open SGLV caused by such parameters as the number of cylindrical sectors, structural curvature and the ratio of rise to span was investigated. Finally, some useful conclusions on the local wind pressure distribution on the structural surface and the wind-induced vibration coefficients of SGLV were developed.     

OFDM通信PAPR性能改善的概率类算法研究

OFDM通信系统是正交多载波系统,很容易造成较高峰均功率比PAPR,从而严重降低OFDM系统的通信质量.文中分析了PAPR~]"OFDM通信性能的不利影响,探讨了改善PAPR性能的SLM.PTS概率类算法原理.基于Matlab软件构造了嵌入SLM,PTS算法模块的OFDM仿真验证系统,并提出了一种PTS改进算法.理论分析与仿真结果表明:SLM,PTS算法可有效减小PAPR OFDM通信性能的不利影响;PTS改进算法可在有效减小PAPR不利影响的前提下,减小OFDM发射机的复杂性.     

浮动车数据在城市宏观交通特性研究中的应用

回顾和总结了国内外浮动车数据在交通信息服务和路网评价方面的研究及应用现状,提出浮动车数据的三阶段应用.重点阐述了面向城市宏观交通运行规律分析的第三阶段应用,提出基于历史交通流状态数据的数据分析流程.并以北京市浮动车系统采集的数据分析结果为例,分析了北京市路网运行速度的时空分布特性以及不同等级道路的里程利用情况.     

营养宣教对西安某社区成年人营养知识-态度-行为的影响

目的了解西安某社区成年人的营养知识水平,帮助其树立合理营养、平衡膳食的理念,以促进健康。方法随机抽取西安市某社区136名成年人,在进行多种形式的营养宣教前后,进行营养知识、态度、行为(knowledge-attitude-practice,KAP)调查,比较宣教前后调查对象对问卷中营养问题的知晓率、对营养教育的态度以及饮食行为的变化来评估营养宣教的效果。结果营养宣教后,该社区成年人的营养知识得以提高,饮食理念和饮食行为趋于合理。结论营养宣教可提高社区成年人的营养知识,能改善他们的饮食理念和饮食行为,在社区开展营养宣教活动是十分必要的。     

Apoptin原核表达载体的构建及在大肠杆菌中的表达

目的构建Apoptin的原核表达载体,并制备抗原物质Apoptin融合蛋白。方法在获得Apoptin融合基因的基础上,成功构建了Apoptin的高效原核表达载体pET-28a( )-Apoptin,将该质粒转化至大肠杆菌E.coliBL21(DE3)受体菌中,以IPTG对其进行诱导表达,聚丙烯酰胺凝胶电泳分析目的蛋白。结果转化有Apoptin的原核表达载体pET-28a( )-Apoptin的大肠杆菌E.coliBL21(DE3)经IPTG诱导后,经SDS-PAGE分析,在相对分子质量约17 000的位置出现目的蛋白条带,大小与Apoptin融合蛋白一致。结论Apoptin原核表达载体pET-28a( )-Apoptin能够表达出Apoptin融合蛋白,为进一步的Apoptin研究和制备Apoptin抗体奠定了基础。     

汽车非确定性故障诊断模型研究

针对汽车非确定性故障诊断特点,基于模糊数学理论,提出了由概率统计法及专家优序法综合确定模糊隶属度的方法,结合对故障程度的描述,建立汽车非确定性故障诊断数学模型。以此为基础,有选择地借鉴模糊数学理论中的模式识别方法对故障进行诊断,最后以实例验证了该模型的实用性。     

基于顾客忠诚度的品牌价值研究

以问卷调查法和因子分析法为基础,探讨了品牌价值和顾客忠诚度的关系,认为顾客忠诚度的提高能够有效提升企业的品牌价值;并提出了基于顾客忠诚度的品牌价值提升策略。     

体外大鼠成骨细胞对破骨细胞形成的影响

目的通过将鼠成骨细胞与破骨细胞直接共培养的实验方法,研究成骨细胞对破骨细胞分化及功能成熟的影响。方法按照M-CSF 30μg/L、RANKL 50μg/L的浓度对鼠骨髓单个核细胞诱导培养6 d,与原代培养3 d的鼠成骨细胞按细胞数量1∶1直接共培养,加入1,25-(OH)2D31×10-8mol/L和PGE21×10-6mol/L,利用形态学观察、TRAP染色、骨吸收陷窝检测等方法对共培养细胞进行鉴定。结果成骨细胞与破骨细胞共培养时,成骨细胞有明显的生长优势;染色后显微镜下可见大量呈单层排列的成骨细胞,偶见TRAP( )的破骨细胞。结论成骨细胞对破骨细胞分化及功能成熟的影响与两者的相对数量有关。本实验中,由于成骨细胞快速生长,当其数量多于破骨细胞时,可能使1,25-(OH)2D3对成骨细胞RANKL表达的上调作用不足,且PGE2对成骨细胞OPG表达的下调作用不足,从而主要表现为对破骨细胞形成及分化的抑制作用。