基于主线拥堵场景的快速路级联信号控制方法

城市快速路高峰时段已经呈现出常态性拥堵,对快速路主线拥堵进行疏导尤为必要.本文通过在主线和入口匝道进行三级交通检测判别,设计了多级联动的信号控制流程与实现方式,构建了不同拥堵程度下的分级响应控制策略,基于瓶颈点通行能力最大化下的实时信号控制算法及最大排队长度限制下的实时信号控制算法,建立了主线及匝道车道开关闭、汇合处信号灯控制、主线动态限速、交通信息诱导等于一体的快速路级联信号控制方法.仿真分析结果表明,在主线不同拥堵程度下采取的级联信号控制方法,可以有效提升快速路主线平均车速,并未导致匝道排队的恶化,局部路网平均延误均有明显降低.     

在地下水压高的未胶结地层中采用新方法修建城市分水隧洞

计划用作控制洪水的国分河分水渠直接在几乎不允许有地表沉降的城区下穿过，并通过有高地下水位且未胶结的成田砂层，因此存在工作面可能塌方从而加剧地表沉降的巨大风险。这一插板法利用钻进钻头，被作为ＡＢ超前桩法。它可以防止隧洞工作面塌方并控制地表沉降，从而证实它是在未胶结地层的城市隧洞施工中可提供插板支承的有效方法。     

浅谈减振器外特性

减振器的外特性是评价减振器性能的重要指标之一。分析了示功特性和速度特性的设计要求，阐述了外特性各种阻尼力的影响因素和外特性曲线畸变的产生原因，同时提出了解决的方法。     

基于预测电流控制的四象限变流器研究

研究了预测电流控制四象限变流器的MATLAB建模与仿真方法,分析了变流器在牵引工况和再生制动工况时的特性。仿真和基于TMS320F2812试验样机的研究结果验证了该控制算法不仅能有效抑制注入电网的谐波电流,获得稳定的直流输出电压,实现了单位功率因数和能量双向流动,还具有良好的瞬态和稳态特性。     

高校图书馆人性化服务浅说

把人性化的理念,引入图书馆管理,对图书馆人性化服务的理念、人性化服务的可行性以及人性化服务的内容与措施作了一定的阐释与论证,目的在于在高校图书馆推广人性化服务的管理模式.     

THE STUDY OF MICROSATELLITES ALTERATION IN DIAGNOSES OF BLADDER CANCER

Objective To investigate the possibility of microsatellite alteration （MA） in diagnosis of bladder cancer of Chinese people, and find the better panel which will be used in clinic. Methods A total of 6 and 10 microsatellite markers were chosen, PCR-SSLP silver staining assay was performed in 31 and 32 bladder cancers tissue, exfoliate cells in urine and 10, 15 non-bladder cancers exfoliate cells in urine, respectively. Results MA （＋） was found in 28 out of 31, 30 out of 32 bladder cancers, and the sensitivity was 90. 3%, 93.7% respectively. The MA of urine sediment of 25 non-bladder cancers was negative, and the specificity was 100%. The cytology was carried out among 19 out of 31, 20 out of 32 bladder cancers at the same time, 2 cases （10.3%） and 3 cases （15%） were found cancer positive, and the sensitivity is significantly lower than that by the analysis of MA in exfoliated cells. Conclusion MA was not associated with grade and stage of the bladder cancer. MA assay is a sensitive and effective method for the early detection of bladder cancer and post-operation surveillance.     

镓盐对维甲酸致骨质疏松大鼠骨代谢及血清微量元素的影响

目的　观察镓盐对维甲酸致骨质疏松大鼠模型骨代谢及血清中相关元素的影响。方法　用维甲酸建立大鼠骨质疏松模型后 ,实验分为 4组 :正常对照组、骨质疏松组、雌激素治疗组、氯化镓治疗组 ,治疗 2个月 ,观察治疗前后模型动物骨代谢及血清中相关元素的改变。结果　镓盐治疗后 ,大鼠平均骨小梁宽度、平均骨皮质厚度、骨小梁百分比均增加 ,TRAP和AKP活性降低接近正常组水平 ,血清镓含量明显升高 ,其他元素未见明显改变。结论　镓盐具有抑制破骨细胞、改善骨质疏松症的骨基质代谢状态、维持微量元素平衡的功能     

一种改良式质粒DNA提取方法的建立与应用

目的建立一种稳定、可靠的质粒DNA提取改良方法,探讨其在分子生物学实验研究中的应用价值。方法将含有目的质粒的菌株扩增后,运用改良后的碱裂解法进行质粒DNA小量提取,微量核酸仪测定提取质粒DNA的产量及纯度;采用琼脂糖凝胶电泳、内切酶酶切及质粒转染真核细胞鉴定提取质粒DNA的质量及转染效率。结果改良式质粒提取方法获得质粒DNA产量为3.2 mg/L菌液,A260/280=1.91;内切酶酶切完全;质粒DNA以超螺旋结构为主;真核细胞转染效率为20%左右。结论改良式质粒DNA抽提方法操作简单、实用,获得质粒DNA产量多、质量高,能达到分子生物学常规实验的要求。     

REAL-TIME DETECTION OF SURVIVIN mRNA EXPRESSION IN CERVICAL CANCER CELL LINES USING MOLECULAR BEACON IMAGING

Objective To detect the expression of survivin mRNA in cervical cancer cell lines using molecular beacon imaging technology. Methods Human cervical cancer cells （HeLa and SiHa） and human fetal lung fibroblast HFL-I were cultured in vitro. After adding 100 nmol/L survivin mRNA molecular beacon, the fluorescent signals were observed under fluorescent microscope. The expressions of survivin in cervical cancer cells and HFL-I cell were examined by immunocytochemical streptravidin-biothin peroxidase （SP） assay at the same time. Results Two kinds of survivin mRNA molecular beacon, with different color fluorescence, had strong fluorescent signal in cervical cancer cell lines, and the signal in SiHa cell line was stronger, but these signals were not found in HFL-I ; Immunocytochemical staining of positive survivin was located in the cytoplasm of cervical cancer cell lines HeLa and SiHa, whereas, no expression of survivin was detected in HFL-I cell line. Conclusion The technology of molecular beacon imaging can be used to detect the expression of survivin mRNA in viable cells successfully, and may provide a new approach to the diagnosis of early stage cervical cancer and the following-up in the clinic.     

人微小病毒B19及硒与大骨节病关系的研究

目的　研究人微小病毒B19(B19)感染和硒与大骨节病的关系。方法　采用聚合酶链反应 (PCR)及荧光法检测大骨节病病区儿童血清B19DNA和红细胞硒含量 ,并与非病区健康儿童进行对比分析。结果　大骨节病病区儿童血清B19感染率显著高于非病区儿童 ,红细胞硒含量显著低于非病区儿童 ,低硒营养状态的儿童B19感染率明显高于正常硒水平的儿童。结论　我国大骨节病病区存在着B19感染的流行 ,低硒可能是B19感染发生和发展的条件。