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51.
The European Community Transport Policy: Towards a Common Transport Policy. By Jurgen Erdmenger (Gower Publishing Co. 1984) [Pp. 155.] £14.50. Transport Reform: Changing the Rules. By Stephen Plowden. P.S.I. No. 642 (London: Institute, 1985.) [Pp. 250.] £6.50. ISBN 0–85374–258–8. The Atlas of British Railway History. By Michael Freeman and Derek Aldcroft (London: Croom Helm, 1985.) [Pp. 128.] £12.95. Losing Track. By Kerry Hamilton and Stephen Potter (Routledge & Kegan Paul in association with Channel Four, 1985.) [Pp. viii + 152.] £7.95. Paperback. 相似文献
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Bacterial pore-forming toxins (PFTs) are essential virulence factors of many human pathogens. Knowl- edge of their structure within the membrane is critical for an understanding of their function in pathogenesis and for the development of useful therapy. Atomic force microscopy (AFM) has often been employed to structurally interrogate many membrane proteins, including PFTs, owing to its ability to produce sub-nanometer resolution images of samples under aqueous solution. However, an absolute prerequisite for AFM studies is that the samples are single-layered and closely-packed, which is frequently challenging with PFTs. Here, using the prototypical member of the cholesterol-dependent cytolysin family of PFTs, perfringolysin O (PFO), as a test sample, we have developed a simple, highly robust method that routinely produces clean, closely-packed samples across the entire specimen surface. In this approach, we first use a small Teflon well to prepare the supported lipid bilayer, remove the sample from the well, and then directly apply the proteins to the bilayer. For reasons that are not clear, bilayer preparation in the Teflon well is essential. We anticipate that this simple method will prove widely useful for the preparation of similar samples, and thereby enable AFM imaging of the greatest range of bacterial PFTs to the highest possible resolution. 相似文献
54.
This research attempted to analyze nanoparticles and other harmful exhaust emissions in accordance with injection strategies and air-fuel ratio (AFR) changes for small diesel engines. The emission characteristics were analyzed in the medium-speed condition, which is the main driving range of a diesel engine. In the case of particulate matter (PM), the number of particles was measured, analyzed, and compared to identify the correlation and emission characteristics of nanoparticles by using a dilution device and condensation particle counter (CPC), which are international standards for particle measurement recommended by the Particulate Measurement Programme (PMP). The engine torque tended to be reduced as pilot injections were added, and the torque was increased by the increased boost pressure, but reduced by the exhaust pressure increase in a part of the low-load range. The number of nanoparticles was not influenced greatly by the change in AFR, but the reduction effect on the PM weight was great depending on the boost pressure increase. In addition, the number of nanoparticles tended to increase as the fuel injection timing became closer to TDC in all conditions, and its difference became larger with an increase in AFR. In addition, in the case of the pilot injection, nanoparticle emission showed similar characteristics depending on the main injection timing, but it was increased by advanced injection timing when performing the main injection only, and the number of the nanoparticles increased as pilot injections were added. Last, the optimal conditions for EMS calibration were analyzed by selecting the conditions of torque reduction and NOx increase within 5 % from all of the engine operating conditions; optimized conditions are presented. 相似文献
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阐述甘泉铁路车站分布和运量发展情况及货运量增加后区间通过能力不足的问题,介绍甘泉铁路行车组织和区间通过能力计算方法,计算各区间的通过能力,结合近期货物运量500万t、700万t、1000万t三种情况,计算需求通过能力,并给出提高通过能力的具体方案,为下一步货运量的增加做准备。 相似文献
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使用有限元分析以改善重载运输车轴 总被引:1,自引:0,他引:1
分析了北美铁路货运发生的车轴故障,介绍了使用有限元对F级和K级车轴进行应力分析计算,研究车轴尺寸变化对应力的影响,并提出了改善重载车轴设计的建议. 相似文献
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Upon the expiry of the concession contract signed between Lebanon and a private company for the Port of Beirut operation (in December 1990), the Government appointed a temporary commission to operate the port under the rules of the expiring concession. The legality of this measure has since always been questioned, while the temporary commission underwent three major personnel changes which clearly prevented any long-term thinking or planning. In 1998, the temporary commission entered into a 20-year joint venture for the establishment of a container terminal. Meanwhile, Lebanon's two main ports were facing major regional competition, while competing against each other to some degree. Thus, the need became urgent to end the temporary status and propose reforms to the entire sector. To that end, the Ministry of Public Works and Transport was commissed by the Government to propose a permanent institutional framework for the port of Beirut while assessing the need for a national maritime sector regulator. This paper presents the recommendations for the institutional reform of the Lebanese maritime transport sector. 相似文献
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A time-domain technique for estimating wave-induced ship hull bending moment from ship motion measurements is presented. This technique is developed to improve hull response monitoring systems. Artificial neural networks are used to model the time-domain relationship between the wave-induced vertical bending moment and the coupled heave and pitch motions. The application of the proposed technique is validated using experimental data. 相似文献