A New Technique of High-performance and Fast Tensioning Prestressed Anchorage CableEI北大核心

Research purposes: The large-span transition section tunnel of the Badaling Great Wall station on the Beijing-Zhangjiakou high-speed railway with the maximum excavation width of 32.7 m, and the largest excavation area of 494.4 m 2 , is the world's biggest traffic tunnel in the world with the largest excavation width and excavation area, which has difficult construction and high security risks. The initial support system of tunnel is mainly realized by prestressed bolt, prestressed cable and shotcrete. After test, anchor cable tension using the traditional anchor cable construction technology can't meet the design requirements, at the same time, it takes about 30 days to achieve prestressed tensioning. Therefore, we need to study the high-performance fast tensioning prestressed anchor cable technology, to effectively control surrounding rock deformation, to ensure the safety of construction, improve construction efficiency. Research conclusions:(1) The traditional anchor cable construction technology is adopted. The anchor cable tension value is mainly controlled by the grip force between the anchor rope and grouting body and the cohesive force between grouting body and surrounding rock. (2) The grip force between the anchor rope and the grouting body can be increased by about 2 times by increasing the "barb"; The cohesive force between the grouting body and the surrounding rock can be increased by 1.5 times by 6 ~ 7 MPa high-pressure grouting process. (3) The modified sulphoaluminate cement slurry can reach more than 30 MPa within 1 day of the slurry strength, so as to realize fast anchor cable tension within 1 day after grouting completion. (4)The research results can be used for reference in similar prestressed anchorage cable construction projects. © 2018, Editorial Department of Journal of Railway Engineering Society. All right reserved.     

轨道车辆直流牵引电机噪声的分析与研究

本文在城市轨道车辆牵引电机噪声测试的基础上,分析了牵引电机噪声的产生机理和频率分布以及对室内外环境的影响和传播途径。最后提出了牵引电机的降噪措施。     

TNFα增加血管内皮细胞通透性与其激活P38 MAPK和黏附因子重新分布有关

目的　研究肿瘤坏死因子 (TNFα)对血管内皮细胞通透性的影响及其机理。方法　采用生物素标记白蛋白的酶联免疫吸附法测定人脐静脉内皮细胞 (HUVEC)单细胞层的通透性 ;免疫荧光、激光共聚焦显微镜和蛋白免疫印迹等方法测定血管内皮黏附因子 (VEcadherin)的分布 ;功能性激酶试验测定有丝分裂原激活蛋白激酶 (MAPK)的活性 ,SB2 0 2 190 (P38抑制剂 )与PD980 5 9(ERK抑制剂 )复合物用于其活性的抑制实验。结果　与对照组比较 ,TNFα明显增加血管内皮细胞的通透性、降低细胞膜VEcadherin的表达 ,诱导P38和ERKMAPK的活性 (P <0 .0 5 )。有趣的是SB2 0 2 190明显降低了TNFα对血管内皮通透性和细胞膜VEcadherin表达的作用 ,而PD980 5 9不能阻止TNFα产生的上述作用。结论　TNFα增加血管内皮细胞的通透性是通过激活P38MAPK信号系统进而抑制VEcadherin在细胞膜的表达和重新分布。     

FRP材料在海港工程中的应用

海工建筑物中的钢筋锈蚀已成为全世界普遍关注并日益突出的一大问题。FRP筋因其优越的防腐性、抗拉强度高、重量轻、总体使用成本低等特点,成为海工结构中钢筋的理想替代品。文中在分析有关资料的基础上,综述了国内外对FRP材料及其在土木工程中应用的研究,并结合海港码头结构的特点,提出FRP材料在海港工程应用中的关键技术,展望FRP应用前景。     

THE EFFECT OF VITAMIN C INTAKE ON GLYCOHEMOGLOBIN MEASUREMENTS

ＴＨＥＥＦＦＥＣＴＯＦＶＩＴＡＭＩＮＣＩＮＴＡＫＥＯＮＧＬＹＣＯＨＥＭＯＧＬＯＢＩＮＭＥＡＳＵＲＥＭＥＮＴＳＦａｎｇＺｈｉｙｕ；ＳｕＭｉｎｇ，Ｒ．Ｒ．Ｌｉｔｔｌｅ，Ｈ．Ｍ．Ｗｉｅｄｍｅｙｅｒ，Ｄ．Ｅ．Ｇｏｌｄｓｔｅｉｎ（ＤｅｐａｒｔｍｅｎｔｏｆＣｈｉ...     

船闸闸室墙表面修复砂浆物理力学性能试验研究

对丙乳砂浆、硅粉砂浆、高强砂浆、聚丙烯纤维高强砂浆及玄武岩纤维高强砂浆5种船闸闸室墙表面修复材料进行了一系列物理力学性能试验。研究结果表明：丙乳明显降低了砂浆的抗压、抗折尤其是抗压强度,但能有效改善砂浆的早期抗干缩能力及增强砂浆韧性：由于硅粉微粒的填充作用及火山灰反应硅粉砂浆具有优良的力学性能,其28d抗压、抗折和抗拉强度分别为分别为85．9MPa、15．93MPa和5．732MPa,能承受的抗弯荷载也较大,为5.3kN,但其塑性收缩大且韧性不强;聚丙烯纤维和玄武岩纤维高强砂浆具有较高的力学性能,同时纤维的加入能有效改善砂浆的早期抗干缩能力．显著增加砂浆的断裂挠度和韧性从而提高砂浆抵抗变形开裂的能力。     

猴细小病毒Vp2的克隆、表达和鉴定

目的　克隆、表达和鉴定猴细小病毒 (simianparvovirus,SPV)Vp2蛋白。方法　用设计的SPVVp2区的特异性引物 ,PCR法扩增SPVVp2基因DNA片断 ;将获得的基因片断插入 pThioHisA载体中 ,转化大肠杆菌DH5α后挑选阳性克隆 ,利用限制性内切酶酶切和核酸序列分析技术鉴定重组质粒 ;以LB培养液培养转化有插入SPVVp2基因的pThioHisA载体的大肠杆菌 ,以终浓度 1mmol·L -1的IPTG诱导蛋白的表达 ;用SDA PAGE和Westernblot分析和鉴定表达的蛋白。结果　经限制性内切酶酶切和核酸序列分析 ,获得了插入pThioHisA载体框架的SPVVp2基因的表达质粒 ;阳性转化大肠杆菌在IPTG诱导下获得了SPVVp2蛋白的表达 ;SDA PAGE和用抗 Thio及抗 SPVVp2的特异性抗体的Westernblot分析证实了表达蛋白的特异性。结论　获得了SPVVp2基因的表达质粒并在大肠杆菌中得了高效表达 ,为进一步建立SPV感染的检测方法和研究SPV的血清流行病学等奠定了基础。     

THE NEURONS DOUBLE-LABELLED BY SEROTONIN AND GLUTAMATE-IMMUNOREACTIVITY IN

ＴＨＥＮＥＵＲＯＮＳＤＯＵＢＬＥ－ＬＡＢＥＬＬＥＤＢＹＳＥＲＯＴＯＮＩＮＡＮＤＧＬＵＴＡＭＡＴＥ－ＩＭＭＵＮＯＲＥＡＣＴＩＶＩＴＹＩＮＭＥＤＵＬＬＡＲＹＲＡＰＨＥＮＵＣＬＥＩＰＲＯＪＥＣＴＩＮＧＴＯＴＨＥＣＥＲＥＢＥＬＬＡＲＣＯＲＴＥＸＯＦＴＨＥＫＩ...     

EFFECTS OF NITRIC OXIDE SYNTHESIS INHIBITION WITH OR WITHOUT NITRIC OXIDE IN

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