THE HUMORAL AND CELLULAR IMMUNE RESPONSES INDUCED BY HPV18L1-E6/E7 DNA VACCINES IN MICE

Objective To construct eukaryutic expression vector of HPV18 L1- E6, E7 chimeric gene and examine the humorul and cellular immune responses induced by this DNA vaccines in mice. Methods The C-terminal of major rapsid protein L1 gene and mutant zinc finger domains of early E6/7 oncogenes in HPV18 were integrated and inserted into eukaryotic expression vector pVAX1 to generate vaccines pVAX1-L1E6Mxx, ETMxx. CHO cells were transiently transfected with the individual construct. Target protein expressions in the lysate of the transfected cells were measured by ELISA and immunocytochemistry After BALB/c mice were vaccinated with various recombinant plusmids（pVAX1- L1-E6M3 or pVAX1-L1-E7M3 ） and immunie adjuvants （pLXHDmB7-2 or LTB） through different administration routes （intramuscular or intranasal） , the great cellular immune responses were produced us revealed by delayed-type hypersensitivity （DTH） and lymphocyte proliferation, and the expression of IL-4 and IFN- 7 cells in CD4^＋ and CD8^＋ subpopulations. Results The highly efficient expression of pVAX1-L1E6Mxx, E7Mxx vector in host eukaryotic cells were demonstrated both by ELISA and immunocytochemistry. The level of specific serum IgG against HPV in experiment groups mice was much higher than that of control group, and intranuscular immunization group had the highest antibody level. Intramuscular immunization groups were superior to intranasal immunization groups in DTH response, splenocyte proliferation and CD8^＋ IFN-γ^＋ cells number, but CD4^＋ IL4^＋ cell number was higher in intranasul immunization groups. The immunization groups using pLXHDmB7.-2 as adjuvant were superior to other groups in immunorespouse. Conclusion These DNA vaccines produce remarkable cellular and humorul immune responses in the mouse and may provide us prophylatic and therapeutic candidates for HPV induced cancer treatment.     

应用免疫组织化学方法评价组织芯片的可靠性

目的探讨组织芯片技术在免疫组化试验中的可靠性和提高组织芯片可信性的方法。方法选择82例人乳腺癌标本石蜡蜡块,利用传统制片技术和自行研制的专用器具分别制作常规石蜡切片和组织芯片,采用免疫组化技术检测雌激素受体(ER)、孕激素受体(PR)的表达。结果采用自行研制的专用器具制备组织芯片,所得样本的可分析率均在92.9%以上,且随着对同一标本取材数量的增加,其可分析标本率也明显上升。采用传统方法检测的82例乳腺癌标本中ER和PR的阳性率分别为61%和58.5%;采用组织芯片技术,一式一份取材,其阳性率分别为51.9%和50.0%,一式两份方式取材,其阳性率分别为53.8%和53.8%,采用一式三份方式取材,其阳性率分别为57.1%和60.7%,且三种不同取材方式的检测结果与传统制片法之间并无统计学差异(P>0.75)。结论在采用统一制备标准并保证组织芯片质量的前提下,挖取直径1.5mm的组织样本制备组织芯片,尽管组织芯片技术与传统方法检测的结果的一致率仍随着对同一标本取材次数的增加而提高,但是三种不同取材方式的检测结果之间并无统计学差异。因此,一式一份取材制备组织芯片完全可替代传统制片技术用于免疫组织化学乃至原位杂交、荧光原位杂交技术的研究,且可保证组织芯片的可信性和高通量特征。     

Empirical speed models for cycling in the Oslo road network

Transportation - Knowing the speed at which a cyclist travels is important in route and mode choice modelling. Empirical evidence suggests that it varies significantly in accordance...     

A differential evolution approach for the vehicle routing problem with backhauls and time windows

This paper presents a differential evolution algorithm (DEA) to solve a vehicle routing problem with backhauls and time windows (VRPBTW) and applied for a catering firm. VRPBTW is an extension of the vehicle routing problem, which includes capacity and time window constraints. In this problem, customers are divided into two subsets: linehaul and backhaul. Each vehicle starts from a depot and goods are delivered from the depot to the linehaul customers. Goods are subsequently brought back to the depot from the backhaul customers. The objective is to minimize the total distance that satisfies all of the constraints. The problem is formulated using mixed integer programming and solved using DEA. Proposed algorithm is tested with several benchmark problems to demonstrate effectiveness and efficiency of the algorithm and results show that our proposed algorithm can find superior solutions for most of the problems in comparison with the best known solutions. Hence, DEA was carried out for catering firm to minimize total transportation costs. Copyright © 2013 John Wiley & Sons, Ltd.     

Man and his transport behaviour Part 4. Fare strategies for public transport—a problem outline

Abstract

In April 1983, the “Fourth World Conference on Transport Research” was held in Hamburg. For a period of four days, experts from all parts of the world discussed transport research and planning problems. The discussions were divided into eight so‐called ‘sub‐topics’. Fortunately, one of the sub‐topics, ‘Man and his Transport Behaviour’ (chaired by Moshe Ben‐Akiva, U.S.A. and Werner Brög, Federal Republic of Germany) dealt with the individual and his behaviour. This complemented the traditionally supply‐oriented thinking of the transport planners by introducing the demand component which had frequently been neglected in the past. Since the view has become increasingly widespread that transport is meant to serve people, and thus, that research should emphasize the (potential) users of the transport system, the number of papers submitted and presented on this sub‐topic was especially large.

However, the number of papers which could be included in the ‘Conference Proceedings’ was limited and this would have meant that a number of interesting documents could not be published. Therefore, papers on four special areas within this sub‐topic are to be published in four consecutive issues of Transport Reviews. The areas which will be dealt with are ‘Telecommunications’, ‘Non‐Motorised Transport’, ‘Special Problems in Third World Countries’ and ‘Fare Structures in Public Transport’. The papers were selected strictly according to their contents. A brief commentary in each issue explains in turn the reasons for choosing each of the four subject areas.

Parts 1–3 appeared in Transport Reviews, 4, 99–113, 173–212, 273–298.     

Superpave技术应用于醴茶高速公路沥青路面的可行性分析

在介绍Superpave技术和分析马歇尔设计方法存在问题的基础上,分析了Superpave技术应用醴茶高速公路沥青路面的可行性。应用Superpave理念对醴茶高速路面结构进行了优化,对比了优化方案的经济性,同时对Superpave的施工要点进行分析,以保证Superpave技术在施工中能够得到良好实现。     

Potential of Real-Time Cylinder Pressure Analysis by Using Field Programmable Gate Arrays

In this paper, a Field Programmable Gate Array (FPGA) was used to implement a real-time cylinder pressure analysis. The goal of the project was to improve the accuracy of calculated heat release and center of combustion calculations to enhance the precision of engine control functions. Compared to today’s real-time pressure analysis systems, several additional physical effects were taken into account for this objective. The wall heat transfer was calculated based on the approach published by Hohenberg. A chemical equilibrium with six substances was assumed for the mixture composition and a real-time calculation method was developed. Furthermore, a two-zone model was adapted and implemented for this realtime analysis. The validation of the results and the rating of the improvement in precision were based on GT-SUITE simulation results as an offline reference tool. Compared to state-of-the-art analysis systems, it was possible to reduce the average error of the center of combustion position from 1.6° to 0.5° crank angle (CA) by taking the investigated effects into account. Moreover, it was possible to significantly reduce the time required for the calculation from one complete combustion cycle to 0.2°CA at an engine speed of 3,000 rpm by using a continuous calculation method on the FPGA. This led to an additional improvement of the ability to control the engine, especially under highly dynamic operation conditions.     

基于玻璃纤维锚杆微应变测试的软岩隧道松动圈测试技术及应用

为解决软弱破碎围岩地层隧道松动圈测试的难题,分析现有松动圈测试技术现状,以全长黏结型玻纤锚杆为载体,提出一种适用于破碎软岩隧道的围岩松动圈测试技术。该测试技术以锚杆微应变变化趋势为基础,结合锚杆中性点理论,可大致确定围岩松动圈范围。将该技术应用在大（理）临（沧）铁路杏子山隧道破碎炭质板岩大变形地段进行验证,结果表明： 全长黏结型玻璃纤维锚杆在松动区可与软弱破碎围岩有效协调变形,锚杆微应变变化趋势与中性点理论相符,采用锚杆微应变变化趋势表征围岩松动圈范围合理可行。同时该技术测试精度可通过调整沿锚杆长度粘贴应变片的密度来控制,有效弥补传统物探松动圈测试技术对岩体要求高的问题,在软弱破碎围岩地层中具有较好的操作性和适用性。     

Physical processes in wheel–rail contact and its implications on vehicle–track interaction

Friction within the wheel–rail contact highly influences all aspects of vehicle–track interaction. Models describing this frictional behaviour are of high relevance, for example, for reliable predictions on drive train dynamics. It has been shown by experiments, that the friction at a certain position on rail is not describable by only one number for the coefficient of friction. Beside the contact conditions (existence of liquids, solid third bodies, etc.) the vehicle speed, normal loading and contact geometry are further influencing factors. State-of-the-art models are not able to account for this sufficiently. Thus, an Extended-Creep-Force-Model was developed taking into account effects from third body layers. This model is able to describe all considered effects. In this way, a significant improvement of the prediction quality with respect to all aspects of vehicle–track interaction is expected.     

从凸缘连接到对焊接头的飞跃:应用于汽车制造业的激光器

如果将对焊接头取代于横梁、底座和管道的凸缘和搭接接头以及其他车身上结构件的焊接,能节省多少材料、重量和燃料?当粘合车门钣金件和汽车框架的密封胶水重量包含在总重量中,这个问题就非常值得一提。而这个问题取决于零部件。如果不再使用凸缘搭接,减少的重量能高达几公斤,同时也能节省每部分的安装空间,约高达8～16mm。搭接凸缘设计是两项极具现代车身制造特色的发明逐渐成熟的产物。第一项是诞生于1877年的电阻焊,它是快速、高效和实用的焊接加工方法。第二项是1 954年应