西安汉族人群HLA-DQA1基因座遗传多态性研究

目的　了解西安汉族群体HLA DQA1基因座的基因及基因型分布 ,获得西安汉族群体HLA DQA1群体遗传学数据。方法　采用PERKIN ELMER公司生产的AmpliTypePM试剂盒 ,PCR扩增HLA DQA1基因座 ,与 11个寡核苷酸探针杂交 ,对西安地区汉族 10 2名无关个体HLA DQA1基因座进行基因频率调查。结果　检出 7种等位基因、2 8种基因型 ,经 χ2 检验基因型的观察值与期望值符合Hardy Weinberg平衡定律 (P >0 .5 ) ,其杂合度 (H)、个体识别率 (DP)、非父排除率 (EPP)和多态信息量 (PIC)分别为 0 .8317、0 .9393、0 .786 6和0 .82 5 0。结论　所得到的群体遗传学数据为遗传学、法医学个体识别和亲权鉴定提供依据     

长安县农村中小学生视力10年监测结果分析

目的　研究农村中小学生 10年的视力变化规律 ,为更好地开展学校视力保护工作提供依据。方法　按照国家统一的学生视力检测方法和要求 ,对长安县中小学生 1990～ 1999年视力进行检测 ,对视力均值及视力低下率分别进行统计分析。结果　 10年间男、女学生双眼视力均值均在 5 .0以上 ,视力低下率为 5 .0 %～ 8.3%。男生的视力均值高于女生 ,视力低下率低于女生。视力低下率随学习阶段的上升而上升 ,以小学至初中阶段增幅最大 ,各学习阶段均以轻、中度视力低下为主 ,重度视力低下率随学习阶段上升增长幅度最大。学生左、右眼视力无明显差异。结论　 10年来长安县农村学生的视力发育状况总体上是良好的 ,但视力低下率随学习阶段增长速度加快。建议 :对学生视力低下的预防应从小学阶段开始 ,尤其要重视小学升初中学习阶段的预防工作 ,同时要防止视力低下程度在初中及高中学习阶段加深     

THE INHIBITORY EFFECT OF MELATONIN ON THE GROWTH OF HUMAN BLADDER CARCINOMA T24 CELL LINE

Melatonin(Mel) ,anindoleamineprimarilyderivedfromthepinealgland ,issecretedduringthehoursofdarkness.Melactsasahormonalconduc tionof photoperiodinfluencedbytheseasonanddailyphysiologicalrhythms.Thefunctionofmelinhumansisobscure .However ,melhasattainedprominenceasatreatmentfordisturbedcircadianrhythmsandsleeppatterns ,hasenhancedthefunc tionofimmunitysystem[1] andhasinhibitedtumorgrowth[2 ] .Thepreviousstudieshaveindicatedthatmelcombinedwithotheranticancerdrugscanin creasetheefficiencyanddecre…     

成人界嵴形态与组织结构的观察

目的　观察和测量人右心房界嵴和梳状肌的形态和组织结构 ,以探讨其可能的功能。方法　肉眼观察、测量界嵴与梳状肌的有关形态学数据。利用光学显微镜 ,通过HE、Masson和磷钨酸 苏木素染色 ,对界嵴的细胞形态和纤维方向等进行细致观察。结果　①根据界嵴的隆起状况 ,界嵴可分为 4类 :A .全程隆起 (18.6 % ) ;B .上 2 / 3隆起 ,下1/ 3平坦 (2 7.9% ) ;C .上 1/ 3隆起 ,下 2 / 3平坦 (34.88% ) ;D .全程平坦 (18.6 % )。②界嵴的肌纤维可分深、浅两层 ,深层肌纤维纵行 ,与界嵴长轴平行 ;浅层纤维横行 ,仅见于界嵴的中、下部 ,深 浅层纤维之间互相联系。在界嵴各处可见P样细胞。③根据梳状肌的起止和走行 ,梳状肌可分为 3型 :汇聚型、网状型和混合型。结论　窦房结上、下及其深面借过渡纤维与界嵴的普通肌纤维相互联系 ,界嵴纤维可能与结间冲动传导有关。梳状肌走行方向的多样性 ,可能与临床上心脏电流传导的多向异性有关     

西北地区汉族HLA-A,B基因座单元型分析

目的　调查分析西北地区汉族人群中HLA A ,B基因座单元型频率。方法　采用PCR SSO反向斑点杂交技术对 87个家系 2 76名西北地区汉族个体HLA A ,B基因座进行检查 ,通过HLA以单元型遗传方式遗传的规律归结出单元型。结果　居单元型频率前三位的是A0 2 B4 6、A30 B13、A0 2 B4 0 ;具有明显连锁不平衡的单元型 11种。结论　西北地区汉族人群中HLA A ,B基因座单元型频率与国内其他地区汉族人群相比有其自身特点 ,A30 B13、A0 1 B37、A32 B4 4具有显著的连锁不平衡性     

Five-axis interpolation of continuous short linear trajectories for 3[PP]S-<Emphasis Type="Italic">XY</Emphasis> hybrid mechanism by dual Bezier blending

A novel five-axis real-time interpolation algorithm for 3[PP]S-XY hybrid mechanism is proposed in this paper. In the algorithm, the five-axis tool path for controlling this hybrid mechanism is separated into two sub-paths. One sub-path describes the movement of 3[PP]S parallel kinematic mechanism module, and the other one describes the movement of XY platform. A pair of cubic Bezier curves is employed to smooth the corners in those two sub-paths. Based on the homogenous Jacobian matrix of 3[PP]S mechanism, a relationship between the position errors of every driving joint in hybrid mechanism and the position deviation of the tool tip center point at the moving platform is established. This relationship is used to estimate the approximation error for the corners smoothing according to the accuracy requirement of tool tip center in interpolation. Due to the high computational efficiency of this corner smoothing method, it is integrated into the look-ahead module of computer numerical control (CNC) system to perform online tool path smoothing. By performing the speed planning based on a floating window scheme, a jerk limited S-shape speed profile can be generated efficiently. On this basis, a realtime look-ahead scheme, which is comprised of path-smoothing and feedrate scheduling, is developed to acquire a speed profile with smooth acceleration. A monotonic cubic spline is employed for synchronization between those two smoothed sub-paths in tool path interpolation. This interpolation algorithm has been integrated into our own developed CNC system to control a 3PRS-XY experimental instrument (P, R and S standing for prismatic, revolute and spherical, respectively). A club shaped trajectory is adopted to verify the smoothness and efficiency of the five-axis interpolator for hybrid mechanism control.     

浅谈SolidWorks在《机械制图》教学中的应用

《机械制图》是工科院校的一门专业基础课，对空间想象能力的要求很高，这也是学习该课程的最大障碍。如果按照传统的教学模式，仅依靠有限的机械模型来培养学生的空间想象能力，显然是不够的。在实际教学过程中，结合实际情况引入SolidWorks软件建立三维模型，在培养学生的空间想象和思维能力方面收到很好的教学效果。     

REPORT OF HLA DISTRIBUTION IN 2315 VOLUNTEER DONORS OF CHINESE BONE MARROW BANK FROM NORTHWEST CHINA

HumanLeukocyteAntigen(HLA)system, knownashumanmajorhistocompatibilitycomplex (MHC),playsakeyroleinantigenprocessing,im muneregulationandotheraspects.HLAisalsobeen calledastransplantingantigenasaccuratetypingof HLAonbothpatientanddonorcanincreasedramat i…     

Experimental Investigation on Contribution of CFRP Attachment to Durability of Reinforced Concrete Structure Subjected to Chloride Attack

The function of externally-bonded carbon fiber reinforced polymer （CFRP） in preventing chloride from entering into concrete is verified by experiment. The results show that externally-bonded CFRP can be considered as a part of corrosion prevention system of strengthened concrete structures subjected to chloride ingress, and the contribution of CFRP should be considered in evaluation of durability of reinforced concrete structures with externally-bonded CFRP. With the effective shielding function of CFRP considered, an equation for residual lifetime prediction of concrete structures with externally-bonded CFRP is derived from Fick＇s dispersion law. CFRP has two functions for coastal concrete structures, including strengthening and increasing durability as part of corrosion prevention system.     

榄香烯联合三苯氧胺对乳腺癌MCF-7细胞株的抑制效应

目的观察榄香烯和三苯氧胺对乳腺癌细胞的抑制效应,尤其是两者联合应用有无协同增效作用。方法将MCF-7细胞培养于RPMI1640培养基中,置入37℃,100%湿度,5%(体积分数)的CO2培养箱内。接种细胞并加入实验药物,用CCK-8法检测三苯氧胺联合榄香烯对细胞的增殖抑制效应,用酶标仪在450nm测量每孔吸光度值,计算每组细胞抑制率。结果无毒剂量的榄香烯与治疗剂量的三苯氧胺联合应用,吸光度均值较单用三苯氧胺明显下降,并且相应的细胞抑制效应较单用组明显上升。结论无毒剂量的榄香烯能够使三苯氧胺对乳腺癌细胞的抑制效应显著增强,提示榄香烯与三苯氧胺联合应用具有协同作用。