新型EGFP标记的杆状病毒转移载体的构建及EGFP的制备

目的构建可表达增强型绿色荧光蛋白(EGFP)融合蛋白的昆虫杆状病毒转移载体,利用Sf-9细胞表达、制备EGFP。方法用PCR法从pEGFP质粒中扩增EGFP基因,NcoⅠ/SalⅠ双酶切,克隆入杆状病毒转移载体NcoⅠ/SalⅠ酶切位点之间,获得pFB-EGFP质粒;转化E.coliDH10Bac,通过转座子Tn7的介导,获得重组杆状病毒Ac-EGFP;感染Sf-9昆虫细胞;利用激光共聚焦显微镜和SDS-PAGE观察并检测EGFP的表达。非变性法纯化EGFP。结果荧光显微镜和激光共聚焦显微镜证实感染的Sf-9细胞可表达EGFP;通过SDS-PAGE获得的EGFP,大小为27ku。结论重组pFB-EGFP载体具备表达EGFP的能力,为分子生物学研究提供了一种新型可表达EGFP融合蛋白的昆虫杆状病毒表达载体。     

异丙酚对兔肝缺血再灌注损伤时内皮素-1和一氧化氮的影响

目的探讨异丙酚对兔在体肝缺血再灌注损伤时内皮素-1(ET-1)和一氧化氮(NO)的影响。方法40只家兔随机分为4组,每组10只,分别为缺血再灌注组(A组)、缺血再灌注加生理盐水2 mL/(kg.h)组(B组)、缺血再灌注加异丙酚8mg/(kg.h)组(C组)和假手术对照组(D组)。各组于再灌注后306、0 min测定肝组织和血浆中ET-1与NO水平以及血清谷丙转氨酶(ALT)、谷草转氨酶(AST)的水平,并进行肝组织形态学观察。结果与D组比较,A、B、C组于再灌注后30 min及60 min时,肝组织和血浆中ET-1均呈上升,但C组的上升程度明显低于同时间点的A、B两组(P<0.01);同时肝组织和血清中NO呈下降,但C组的下降程度明显低于同时间点的A、B两组(P<0.01);血清ALT、AST均升高,但C组的升高程度明显低于同时间点的A、B两组(P<0.01);肝细胞形态学发生异常改变,C组的肝脏淤血明显较A、B两组轻,肝细胞变性坏死不明显。结论异丙酚通过保护肝窦内皮,降低机体内ET-1水平,提高NO水平,改善肝脏微循环障碍,对HIRI有保护作用。     

基于频域法的结构疲劳寿命预测方法研究

以Didik方法的雨流循环计数应力范围概率密度函数为依据,考虑长寿命区的S-N曲线,利用不完全Γ函数建立了一种宽带随机载荷谱下结构疲劳寿命计算的解析解法,为用频域法计算结构在宽带随机载荷谱下疲劳寿命提供了一种快捷方法。     

Study on dynamic characteristics of vehicle gearbox under multi-boundary conditions

With the development of vehicle gearbox to high-power-density and high-speed, how to predict and optimize the dynamic characteristics of vehicle gearbox becomes increasingly prominent. Aiming at the vehicle gearbox, this paper comprehensively and deeply studies the dynamic characteristics under the multi-boundary conditions. The generation mechanism of the multi-source excitations triggering the gearbox vibration is analyzed firstly. The vibration transfer path of the gearbox is explored. Secondly, the engine excitation, the gear meshing excitation and the bearing support load are numerically calculated. According to the finite element method, a fluid-solid coupling finite element model of the gearbox body is established to predict the gearbox dynamic responses based on the Galerkin method and the Hamiltonian variational principle. Finally, the effects of the excitation condition, oil height and reinforcement forms on the vibration responses of the gearbox body are thoroughly studied by simulation. The analysis indicates that it not only helps to modify and improve the method of forecasting the gearbox dynamic response, and also provides the theoretical and technical guidance for the gearbox design and optimization.     

Geometric calibration algorithm of polarization camera using planar patterns

Polarization pattern provides additional information besides spectral signatures. It can be used in many applications, such as navigation, defect detection and object identification. A novel polarization camera composed of four synchronized cameras is proposed, and it can realize real-time polarization measurement. This study particularly concentrates on the geometric calibration of the system. The projection model is analyzed and the multi-camera calibration algorithm is proposed. Firstly, each camera is calibrated separately using planar patterns, and then the geometric calibration algorithms are performed. Due to the geometrical constraint, a global optimization method results in smaller estimation uncertainties. A mean rotation error of 0.025° and a mean translation error of 0.26mm are achieved after geometric calibration. The images are rectified to establish a correspondence among cameras and are combined to acquire the polarization measurement. The polarization pattern of the skylight is measured by the system and the results are consistent with the previous studies.     

Research of legal affair simulation based on Petri net

Legal affair simulation is on the basis of information technology, and can simulate legal scene. College students do legal experiments with virtual reality scene. This helps to train college students. Petri net has not only strict math definition to analyze the dynamic behavior of models, but also intuitive graphic expression. Petri net is used to model for legal process. Reduction rules of Petri net are used to analyze the correctness of the system. At last Java Business Process Management (JBPM) technology is used to realize legal affair simulation models, models are transformed into flow charts. Simulation software deduces processes of legal affair. Many college students have improved their ability with the software, the fact proves that the method is effective.     

Compensation of pressure enthalpy effects on temperature fields for throttling of high-pressure real gas

For the pressure enthalpy of high pressure pneumatics, the computational fluid dynamics (CFD) simulation based on ideal gas assumption fails to obtain the real temperature information. Therefore, we propose a method to compensate the pressure enthalpy of throttling for CFD simulation based on ideal gas assumption. Firstly, the pressure enthalpy is calculated for the pressure range of 0.101 to 30 MPa and the temperature range of 190 to 298 K based on Soave-Redlich-Kwong (S-R-K) equation. Then, a polynomial fitting equation is applied to practical application in the above mentioned range. The basic idea of the compensation method is to convert the pressure enthalpy difference between inlet air and nodes into the compensation temperature. In the above temperature and pressure range, the compensated temperature is close to the real one, and the relative temperature drop error is below 10%. This error is mainly caused by the velocity difference of the orifice between the real and ideal gas models. Finally, this compensation method performs an icing analysis for practical high pressure slide pilot valve.     

Hybrid process of fabricating high-quality micro wine-glass fused silica resonators

A new hybrid method, which combines improved glass-blown technology with wet etching, is reported to fabricate micro wine-glass resonators with high-quality fused silica. The optimum placement is compared to achieve the resonators with good shell shape. The typical shell diameter is about 4mm and its thickness covers from dozens to hundreds of micrometers. The etching rates in corrosion solutions with different ratios and at different thicknesses of hemispherical shells are studied. We also conclude how to precisely control the thickness. The corrosion solutions with different ratios of HF solution to NH₄F solution make the spherical shells rougher in different degrees. The best roughness is 0.581 nm in the 1: 8 ratio corrosion solution while the original roughness is 0.537 nm. This fact shows that the resonator remains atomically smooth surface. Based on the glassblowing spherical fused silica structure, the thickness of the resonator is effectively controlled by buffered oxide etch (BOE) technology according to the measured etching rate. The measured resonant frequency of the hemispherical shell at ambient pressure and room temperature is 1.75 kHz of rocking mode which is close to the simulated frequency. Using such a low-cost hybrid approach, we can fabricate high-quality microscale resonators in batch.     

Lattice-based group signature with verifier-local revocation

Among several post quantum primitives proposed in the past few decades, lattice-based cryptography is considered as the most promising one, due to its underlying rich combinatorial structure, and the worst-case to average-case reductions. The first lattice-based group signature scheme with verifier-local revocation(VLR) is treated as the first quantum-resistant scheme supported member revocation, and was put forward by Langlois et al. This VLR group signature(VLR-GS) has group public key size of O(nm log N log q), and a signature size of O(tm log N log q log β). Nguyen et al. constructed a simple efficient group signature from lattice, with significant advantages in bit-size of both the group public key and the signature. Based on their work, we present a VLR-GS scheme with group public key size of O(nm log q) and signature size of O(tm log q). Our group signature has notable advantages: support of membership revocation, and short in both the public key size and the signature size.     

Automatic outer surface extraction of femoral head in CT images

Computer-aided hip surgery planning and implant design applications require accurate segmentation of femoral head and proximal acetabulum. An accurate outer surface extraction of femoral head using marching cubes algorithm remains challenging due to deformed shapes and extremely narrow inter-bone regions. In this paper, we present an automatic and fast approach for segmentation of femoral head and proximal acetabulum which leads to accurate and compact representation of femoral head using marching cubes algorithm. At first, valley-emphasized images are constructed from original images so that valleys stand out in high relief. Otsu’s multiple thresholding technique is applied to seperate the images into bone and non-bone classes. Region growing method and threedimensional (3D) morphological operations are performed to fill holes in the bone. In the reclassification process, the bone regions are further segmented, and the boundaries of the bone regions are further refined based on Bayes decision rule. Finally, marching cubes algorithm is applied to reconstruct a 3D model and extract the outer surface of femoral head and proximal acetabulum. Experimental results show that this method is an accurate segmentation technique for femoral head and proximal acetabulum and it can be applied as a tool in medical practice.