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171.
The past emphasis in this conference series has been on the best ways to deregulate regulated public transport markets. This workshop reverses this process by examining the best ways to regulate deregulated public transport markets. A hierarchy of regulatory needs is identified and three hybrid models examined, based loosely on experience from Great Britain, New Zealand and Sweden. It is argued that deregulated public transport markets are a global phenomenon but regulatory measures should reflect local requirements. The resultant process of glocalisation might result in regulatory measures that focus on the rules of law and their enforcement in emerging public transport markets (such as urban transport in Sub Saharan Africa and for the soon to be competitive inter urban market in Germany) but that focus on guidance for network integration and incentivisation for welfare maximisation in more mature public transport markets (as in Great Britain, New Zealand and Sweden).  相似文献   
172.
This article provides results on the net benefits generated from the natural resources in the Bohol Marine Triangle (BMT) in the Philippines. The BMT spans over 112,000 ha and its coastal ecosystems are rich in biodiversity and provide economic opportunities to the coastal communities. With a 10% discount rate, the accumulated total net benefits for the BMT resources over a 10-year period is US$11.54 million. Tourism and the municipal fisheries are the most important direct use values of the coastal and marine resources of the BMT accounting for 44% and 39% of the total net benefits. Annual revenues attributed to ecosystems were as follows: coral reefs, US$1.26 million; beach/intertidal area, US$1.12 million; marine waters, US$646,501; mangrove, US$239,561; and seagrass, US$105,990. The large market values indicate the dependence of the local community on the BMT coastal and marine resources. In the same way, non-market values show the important life-support functions of coastal and marine ecosystems. The net benefits reflect the magnitude of potential losses due to improper management of coastal and marine resources in the BMT. This valuation highlights the importance of the coastal services to the BMT economy and draws attention to the benefits the local stakeholders derive from BMT coastal resources. Policy measures can now take into account these values to justify a sufficient investment in coastal management efforts to sustain the flow of coastal services in the interest of current and future generations.  相似文献   
173.
Although the success of marine protected areas (MPAs) and associated zoning schemes is largely dependent on stakeholder acceptance and support, there have been relatively few studies examining the response of recreational stakeholders to MPA proposals. This study investigates the response of recreational boaters to a proposed MPA in British Columbia, Canada. Using a structured questionnaire (n = 543) and a focus group (n = 6), the research examines the level of support for the concept of marine zoning and identifies key factors influencing support or opposition. Results indicate that a small majority of recreational boaters support the concept of marine zoning, but that there are areas of concern that ought to be addressed as MPA implementation proceeds. Support for zoning was strongly related to perceived benefits, particularly environmental benefits. Furthermore, perceived benefits overall were found to outweigh concerns as determinants of recreational boater support for zoning. Major dimensions of opposition included perceptions of over-regulation, loss of access for boating, and mistrust of the government. A suggestion is that such concerns may be mitigated through focused communication and meaningful involvement of boaters in the zoning process.  相似文献   
174.
Multidisciplinary, marine ecological observations were conducted at the shallow water edge of the Northeast Water in June, 1993. Although variable in size and shape, a small polynya was constantly present at Eskimonaes, at the fast-ice edge of Ingolfsfjord. A shallow stratified layer developed at the water sufface at negative water and air temperatures—an effect of sea ice melting in cold water early in the season. Nutrients were recorded in considerable quantities, although by mid July NO3 had become depleted. The chlorophyll and phytoplankton maxima at 8–12 m depth had peak values of 2 mg chl a m−3, typical for Arctic algal blooms. The phytoplankton included over 90 species and was dominated by the Fragillariopsis group. Zooplankton was poor in biomass and density, but over 23 taxa were found, with the copepods Oithona similis and Pseudocalanus acuspes being numerically dominant. Sedimentation was approximately 0.2 g dry weight m−2 d−1 and suspended matter concentrations ranged from 4 to 19 mg l−1. The benthos was represented by hard bottom forms only, with a surprisingly dense cover of macrophytes. Juvenile sea urchins (Strongylocentrotus droebachiensis), brittle stars (Ophiocten sericeum) and amphipods were dominant. Higher trophic levels were represented by benthic feeders, such as eiders and walruses. The area observed was more similar to high Arctic fjord ecosystems than to the offshore central Northeast Water polynya.  相似文献   
175.
Gulf menhaden, Brevoortia patronus, which constitutes a major industrial reduction fishery in the USA, spawn across the northern Gulf of Mexico with a focus of spawning about the Mississippi Delta. This species is estuarine dependent; adults spawn over the continental shelf and their larvae are transported, by mechanisms that are presently not well understood, to estuarine nursery areas. Larval gulf menhaden, along with some other surface oriented larval fishes, appear to aggregate along the Mississippi River plume front, while evidence of the ecological consequences of this aggregation in terms of the feeding, growth, and survival of larvae is ambiguous. On an annual scale, Mississippi River discharge is negatively associated with numbers of half year old recruits. Discharge of the Mississippi River and the population recruitment of gulf menhaden may be plausibly linked through the action of the river's plume and its front on the shoreward transport of larvae. Greater river discharge results in an expansive plume that might project larvae farther offshore and prolong the shoreward transport of larvae. An indirect, decadal scale, positive response of recruitment and river discharge is possible, but not certain. Recruitment became elevated after 1975 when river discharge increased and became highly variable. This response might owe to enhanced primary and secondary production driven by nutrient influx from the Mississippi River.  相似文献   
176.
Floating moored offshore structures have a significant future in offshore operations as an attractive economic alternative to fixed structures in deep waters and/or in areas where there is no existing infrastructure. This paper describes an analysis procedure based on the structure variable approach to estimate load and response values of a moored offshore platform at a given return period by taking into account the joint occurrence of wave, wind, and current. The results show that the most severe mooring loads may not occur when wind, wave, and current are collinear and are at their maximum design values, i.e., the 50- or 100-year case. It is recommended that the extreme mooring design loads for moored offshore systems should be determined through a range of physical or numerical simulations where wave, wind, and current are noncollinear and act with less severe magnitudes than the 50- or 100-year case. This recommendation has also been adopted in the ITTC/Ocean Engineering Committee recommendations to the ITTC Conference held in September 1996.  相似文献   
177.
Speed differential has direct relationship with road safety. Conventional speed consistency measures draw sample data from independent population that follows a normal distribution. These methods may fall into ecological fallacy and overestimate the safety level of road elements. After deducing the relationship between the difference in operating speed ΔV85 and85(ΔV), the 85th percentile value of individual speed reduction, this paper recommends 85(ΔV) and speed reduction rate 85(ΔV)R is used to evaluate ramp safety. Point speeds of individual vehicle at freeway diverge area, upper ramp, and lower ramp are collected by radar guns. Safety performance of 37 exit ramps are appraised using three different measures, namely, ΔV85, 85(ΔV), and 85(ΔV)R. The results show that 85(ΔV) is 1.42 to 2.02 times of ΔV85, and that the evaluation result of 85(ΔV)R is between the other two. The measure 85(ΔV)R considers not only individual speed but also the base speed on upstream element, which avoid the pitfalls of ecological fallacy and overestimation possessed by conventional measures. It is a safer and reasonable measure that should be adopted in practice.  相似文献   
178.
High resolution structural studies of DNA and DNA binding proteins by atomic force microscopy (AFM) require well-bound samples on suitably flat substrates. Adsorbing the DNA onto a positively charged supported lipid bilayer has previously been shown to be a potentially effective strategy for structural studies with AFM. Here, using our home-built frequency-modulation AFM (FM-AFM), we show that these bilayer substrates are only maximally effective for high resolution AFM when the samples are short, linear DNA, compared with circular plasmid DNA. We find that, with the former sample, the measured width of the DNA is about 2 nm, the known DNA diameter, and there is a clear height modulation along the length of the DNA with a periodicity of about 3.4 nm, in excellent agreement with the known pitch of the double helix. This sample preparation strategy is expected to enable higher resolution studies of DNA and DNA binding proteins with FM-AFM than that can presently be achieved.  相似文献   
179.
It is important to reconstruct a continuous surface representation of the point cloud scanned from a human body. In this paper a new implicit surface method is proposed to reconstruct the human body surface from the points based on the combination of radial basis functions (RBFs) and adaptive partition of unity (PoU). The whole 3D domain of the scanned human body is firstly subdivided into a set of overlapping subdomains based on the improved octrees. The smooth local surfaces are then computed in the subdomains based on RBFs. And finally the global human body surface is reconstructed by blending the local surfaces with the adaptive PoU functions. This method is robust for the surface reconstruction of the scanned human body even with large or non-uniform point cloud which has a sharp density variation. Foundation item: the National Natural Science Foundation of China (No. 50575139) and the Shanghai Special Fund of Informatization (No. 088)  相似文献   
180.
利用TRIzol(R)试剂和液氮提取大鼠胰腺高质量总RNA   总被引:1,自引:0,他引:1  
目的 建立胰腺高质量总RNA快速、经济、稳定的提取方法.方法 应用TRIzol(R)试剂和液氮提取大鼠胰腺总RNA,通过总RNA含量和A260/280比值的测定及10g/L非变性琼脂糖凝胶电泳评估总RNA的质量,并用RT-PCR检测大鼠胰岛素1、胰高血糖素、胰α-淀粉酶和β-actin的表达.结果 利用TRIzol(R)和液氮提取的大鼠胰腺总RNA量可达到3~6μg/mg胰腺组织,A260/280比值在1.75~1.89之间.在10g/L非变性琼脂糖凝胶电泳时,均可见28S及18S条带,并且在28S、18S条带间可见明显的云雾状阴影.大鼠胰岛素1、胰高血糖素、胰α-淀粉酶、β-actin RT-PCR产物电泳结果显示均有清晰的条带.结论 应用TRIzol(R)试剂和液氮成功地提取了大鼠胰腺高质量的总RNA,后者可用于RT-PCR研究.  相似文献   
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