真核表达人乳头瘤病毒16型L_1蛋白在检测宫颈癌患者相应抗体的应用

用真核表达HPV16L1蛋白为抗原，酶联免疫吸附测定64例宫颈癌患者血清和阴道分泌物的相应抗体，并用型特异性聚合酶键反应检测宫颈癌组织HPV16L1基因。结果：宫颈癌组血清HPV16L1抗体阳性年64．1％，对照组20．8％；宫颈癌组阴道分泌物HPV16L1抗体阳性率64.1％，对照组25％；宫颈癌组HPV16L1基因检出率为42．2％，对照组10％。有显著差异（P＜0．01）。结论：高危型HPV感染，宫颈癌患者有明显免疫反应，全身和局部无明显差异，真核表达HPV16L1蛋白可用于宫颈癌患者的抗体测定。     

On model-based longitudinal feed-forward motion control for low velocities on known road profiles

ABSTRACT

So far, longitudinal motion control has focused on situations like highway driving, where disturbances of the road profile can be neglected. In this paper, we show how the Two Point Tire Model can be used to derive a novel feed-forward control law for a vehicle's longitudinal motion that considers the effects of the road profile and can complement existing control approaches. For this purpose, we recapitulate the basic model assumptions and equations and briefly discuss how it can be used on arbitrary road profiles. Two approaches for implementation in a real vehicle are presented. Comparisons of these approaches in simulation and to a human driver of an experimental vehicle show that the controller can deal with stepped obstacles of up to 14?cm in height. However, the control performance is essentially limited by the actuator delay and human drivers outperform the controller due to their ability of sensing subtle vehicle motions. The results indicate that the control performance can be further improved by using a preview on the necessary drive torque, which can be provided by the solution that we propose.     

Propeller torque load and propeller shaft torque response correlation during ice-propeller interaction

Ships use propulsion machinery systems to create directional thrust. Sailing in ice-covered waters involves the breaking of ice pieces and their submergence as the ship hull advances. Sometimes, submerged ice pieces interact with the propeller and cause irregular fluctuations of the torque load. As a result, the propeller and engine dynamics become imbalanced, and energy propagates through the propulsion machinery system until equilibrium is reached. In such imbalanced situations, the measured propeller shaft torque response is not equal to the propeller torque. Therefore, in this work, the overall system response is simulated under the ice-related torque load using the Bond graph model. The energy difference between the propeller and propeller shaft is estimated and related to their corresponding mechanical energy. Additionally, the mechanical energy is distributed among modes. Based on the distribution, kinetic and potential energy are important for the correlation between propeller torque and propeller shaft response.     

On the perceived justice of urban road pricing: An empirical study in Lyon

Various methods of restricting automobile traffic, by price (tolls) or by quantity (odd/even license plates or limited days of traffic), are tested in a survey (N ～ 400) about attitudes toward traffic restrictions in Lyon, France. Ordered probit models with random-effects panel allow us to estimate the survey respondents’ perceptions of these methods, as well as the roles of individual socio-demographic characteristics in the formation of these perceptions. Both the restriction of automobile traffic and its regulation by congestion (waiting in line) are widely considered unjust by the respondents, regardless of whether they work and whether they are drivers or non-drivers. Their attitudes towards tolls justified by the pollution caused by automobile traffic are less negative. As regards compensation, in addition to emergency vehicles and those that transport people with limited mobility, respondents believe car-pooling ought to benefit of a toll exemption. The support for a reduced rate for low-income users shows a concern for justice to which it will be necessary to respond. The respondents’ socio-professional status, level of education, car use or non-use, and residence inside or outside of the toll zone clearly play a role in their perceptions of these methods of regulation and compensation.     

The role of parents’ mobility behavior for dynamics in car availability and commute mode use

Transportation - Recent studies have analyzed travel behavior over the life course through the lens of the mobility biography approach. Similarities in the effects of certain key events on travel...     

Man and his transport behaviour Part 4. Fare strategies for public transport—a problem outline

Abstract

In April 1983, the “Fourth World Conference on Transport Research” was held in Hamburg. For a period of four days, experts from all parts of the world discussed transport research and planning problems. The discussions were divided into eight so‐called ‘sub‐topics’. Fortunately, one of the sub‐topics, ‘Man and his Transport Behaviour’ (chaired by Moshe Ben‐Akiva, U.S.A. and Werner Brög, Federal Republic of Germany) dealt with the individual and his behaviour. This complemented the traditionally supply‐oriented thinking of the transport planners by introducing the demand component which had frequently been neglected in the past. Since the view has become increasingly widespread that transport is meant to serve people, and thus, that research should emphasize the (potential) users of the transport system, the number of papers submitted and presented on this sub‐topic was especially large.

However, the number of papers which could be included in the ‘Conference Proceedings’ was limited and this would have meant that a number of interesting documents could not be published. Therefore, papers on four special areas within this sub‐topic are to be published in four consecutive issues of Transport Reviews. The areas which will be dealt with are ‘Telecommunications’, ‘Non‐Motorised Transport’, ‘Special Problems in Third World Countries’ and ‘Fare Structures in Public Transport’. The papers were selected strictly according to their contents. A brief commentary in each issue explains in turn the reasons for choosing each of the four subject areas.

Parts 1–3 appeared in Transport Reviews, 4, 99–113, 173–212, 273–298.     

Fixed‐route taxi system: route network design and fleet size minimization problems

This paper introduces the taxi route network design problem (TXRNDP) for a fixed‐route taxi service operating in Iran and, in similar form, in various other developing countries. The service operates fairly similar to regular transit services in that vehicles are only permitted to follow a certain predetermined route on the network. The service is provided with small size vehicles and main features are that vehicles only depart if full and that there are no intermediate boarding stops. In Iran the service attracts a high modal share but requires better coordination which is the main motivation for the present study. We develop a mathematical programming model to minimize the total travel time experienced by passengers while constraining the number of taxi lines, the trip transfer ratio and the length of taxi lines. A number of assumptions are introduced in order to allow finding an exact rather than heuristic solution. We further develop a linear programming solution to minimize the number of taxis required to serve the previously found fixed‐route taxi network. Results of a case study with the city of Zanjan, Iran, illustrate the resulting taxi flows and suggest the capability of the proposed model to reduce the total travel time, the total waiting time and the number of taxi lines compared to the current taxi operation. Copyright © 2016 John Wiley & Sons, Ltd.     

Reducing car dependence in the heart of Europe: lessons from Germany,Austria, and Switzerland

Munich, Berlin, Hamburg, Vienna, and Zurich – the largest cities in Germany, Austria, and Switzerland – have significantly reduced the car share of trips over the past 25 years in spite of high motorisation rates. The key to their success has been a coordinated package of mutually reinforcing transport and land-use policies that have made car use slower, less convenient, and more costly, while increasing the safety, convenience, and feasibility of walking, cycling, and public transport. The mix of policies implemented in each city has been somewhat different. The German cities have done far more to promote cycling, while Zurich and Vienna offer more public transport service per capita at lower fares. All five of the cities have implemented roughly the same policies to promote walking, foster compact mixed-use development, and discourage car use. Of the car-restrictive policies, parking management has been by far the most important. The five case study cities demonstrate that it is possible to reduce car dependence even in affluent societies with high levels of car ownership and high expectations for quality of travel.     

Noise,vibration, harshness model of a rotating tyre

ABSTRACT

The tyre plays a fundamental role in the generation of acoustically perceptible driving noise and vibrations inside the vehicle. An essential part of these vibrations is induced by the road excitation and transferred via the tyre into the vehicle. There are two basic ways to study noise, vibration, harshness (NVH) behaviour: Simulations in time and frequency domains. Modelling the tyre transfer behaviour in frequency domain requires special attention to the rotation of the tyre. This paper shows the approach taken by the authors to include the transfer behaviour in the frequency range up to 250?Hz from geometric road excitations to resulting spindle forces in frequency domain. This paper validates the derived NVH tyre model by comparison with appropriate transient simulations of the base transient model.     

CaPaSIM statement of methods

In the present paper, the method for calculation of the dynamic pantograph–catenary interaction developed by the Royal Institute of Technology and the Swedish National Rail/Road administration (Trafikverket) is described and the results of the benchmark exercise are discussed. The method is based on the commercial Finite Element software ANSYS. The geometry of the catenary and pantograph is defined in a pre-processor, BARTRAD, developed by Trafikverket, and is automatically translated into an ANSYS model. Basically all types of catenary systems could be handled as well as different types of non-linearity. There are both 2D and 3D versions of the code existing. The results achieved in this first stage of the benchmark are well in line with the results from the other partners in the benchmark study