IN VITRO CO-STIMULATORY ACTIVITY OF HUMAN B7.2(IgV+C)PROTEIN PRODUCED BY ENGINEERED BACTERIA

It was well known that "two signals" wereboth necessary to activate T lymphocytes. The firstsignal was provided by TCR--CD, which was en-gaged with antigen peptide--MHC complex, and thesecond, also named the costimulatory signal, wasprovided by the interaction between costimulatorymolecules B7. l and B7. 2 on antigen presenting celland its counter--receptor CD28/CTLA4 on T cell.The first signal alone without costimulation willlead T cell to anergy or apoptosis['~'2. B7. 2 mayplay a cri…     

不同铣刨料掺量对乳化沥青稳定碎石混合料性能影响研究

通过物理力学室内试验,探讨不同铣刨料掺量对乳化沥青稳定碎石混合料性能的影响.结果表明,不掺加铣刨料的混合料最大干密度最大;干湿劈裂强度与空隙率有一定的相关性;不同铣刨料掺量对混合料冻融劈裂强度影响不大;不掺加铣刨料的混合料7d无侧限抗压强度最小;不同铣刨料掺量的混合料在15℃下的破坏应变均大于2800με,具有一定的抗变形性能,可以减少沥青路面的基层反射裂缝.     

The Simulation and Characteristic Study of Wind Velocity for Long-Span Structures

The new technique that combines wave superposition with the fast Fourier transformation was introduced to simulate the nodal three-dimension relevant wind velocity time series of spatial structures. The wind velocity field where the spatial structure is located is assumed to be homogeneous. The wind‘s power spectral density is divided into frequency spectral function and coherency function and the spectral functions are transformed as the superposition coefficients. The wavelet analysis has excellent localized characters in both time and frequency domains, which not only makes wind velocity time series analysis more accurate, but also can focus on any detail of the objective signal series. The discrete wavelet transformation was adopted to decompose and reconstruct the discrete wind velocity time series. The stability of wavelet analysis for the wind velocity time series was also proved.     

沙眼衣原体D型MOMP基因的克隆和序列分析

目的　从D型沙眼衣原体培养物中克隆主要外膜蛋白(MOMP)基因。方法　利用细胞培养法扩增沙眼衣原体后,提取基因组为模板,经聚合酶链反应(PCR)扩增全长MOMP基因,并用酶切、PCR扩增及序列测定等方法对重组质粒进行鉴定。结果　成功扩增了MOMP基因,并插入克隆载体 pUCmT中。结论　MOMP基因的克隆为进一步开展沙眼衣原体的疫苗研究奠定了基础。     

改善城市道路机动车排放污染的智能交通手段

论述了发展智能交通系统 ( ITS)对缓解城市道路交通矛盾 ,减少机动车排放污染的基本原理与控制手段。ITS不仅可提高道路交通运输与管理效率 ,更是一种控制道路交通污染的重要手段。作为该理论的一种实际验证 ,对重庆市拥有 2 .5万辆规模的路桥不停车电子收费系统进行了检测。通过实测表明 :同一路段实施不停车收费与停车缴费相比 ,前者可使机动车排放物 CO降低1 .72倍 ,NOx 可降低 0 .49倍 ,THC可降低 0 .1 7倍 ,改善大气环境质量效果明显。     

客运专线钢系杆拱桥自振特性及结构参数研究

利用大型通用有限元软件,对一座客运专线钢系杆拱桥结合梁桥面方案进行有限元分析。分析结果表明,横撑布置、桥面系刚度、矢跨比和拱肋刚度对竖向基频影响不大,桥面系刚度对横向基频的影响很大,桥面系下平联斜撑和铺设钢板均能显著提高其横向基频。     

柞小公路隧道爆破震动波现场监测与分析研究

依托西(安)康高速公路8标和18标段的施工工程,对小间距隧道围岩爆破开挖施工震动波传递规律进行了现场监测与分析研究,给出了两标段不同围岩级别的爆破震动波速衰减方程,通过分析预测给出了不同围岩级别的爆破安全设计距离。经对千枚岩和灰岩的爆破震动波速分析对比,给出了在不同岩性中波速传递的规律性。通过对振动波速的测试结果分析,获得了不同方向的波速传递规律和爆破震动波的最大影响区域范围和对既有隧道围岩与支护稳定性的影响范围,所获结论可供小间距隧道工程设计与施工参考。     

增强型绿色荧光蛋白标记的Hela细胞株的建立

目的构建增强型绿色荧光蛋白(EGFP)标记的Hela细胞系。方法PCR和DNA测序鉴定pEGFP-C1真核表达质粒,采用Qiagen tip 500进行pEGFP-C1真核表达质粒DNA的提取和纯化;cellfectin转染Hela细胞,G418筛选,有限稀释法单克隆培养,荧光显微镜进行荧光检测EGFP的表达,采用激光共聚焦显微镜观测器检测荧光特性。结果PCR和DNA测序证实pEGFP质粒结构正确;在倒置荧光显微镜下,转染24h后,部分Hela细胞可见绿色荧光,转染72h时,大约80%Hela细胞内均可见绿色荧光。加入含G418的选择性培养基进行选择性培养,选择荧光较强的Hela细胞经有限稀释,持续筛选及克隆化培养,获得稳定表达绿色荧光的Hela细胞克隆,扩大培养并传至10代以上,将此细胞株命名为Hela-EGFP。激光共聚焦显微镜观察发现:在蓝光(-395nm)激发时,Hela-EGFP细胞绿色荧光激发波长为395nm,最大发射峰为509nm。结论Hela-EGFP细胞株具备稳定表达EGFP的能力,为实时可视化进行宫颈癌侵袭转移机制的研究奠定了基础。     

A method to improve first order approximation of smoothed particle hydrodynamics

Smoothed particle hydrodynamics (SPH) is a useful meshless method. The first and second orders are the most popular derivatives of the field function in the mechanical governing equations. New methods were proposed to improve accuracy of SPH approximation by the lemma proved. The lemma describes the relationship of functions and their SPH approximation. Finally, the error comparison of SPH method with or without our improvement was carried out. Foundation item: The National Natural Science Foundation of China (No. 50778111); The Key Project of Fund of Science and Technology Development of Shanghai (No. 07JC14023)     

pSVL/GM-CSF真核表达质粒的构建及其表达活性观察

以真核瞬时表达质粒ｐＳＶＬ为载体构建ｐＳＶＬ／ＧＭ－ＣＳＦ真核表达质粒，用电穿孔法导入Ｃｏｓ－７细胞，以ＧＭ－ＣＳＦ依赖株ＴＦ１为靶细胞，检测其生物学活性；将不同剂量的ＰＳＶＬ／ＧＭ－ＣＳＦ质粒直接注射Ｂａ１ｂ／Ｃ小鼠股四头肌，动态观察小鼠血清中ＧＭ－ＣＳＦ的表达；将ｐＳＶＬ／ＧＭ－ＣＳＦ质粒导入Ｋ５６２细胞，观察ＧＭ－ＣＳＦ对瘤细胞生长状态的影响。结果表明：构建的ｐＳＶＬ／ＧＭ－ＣＳＦ真核表达质粒可在Ｃｏｓ－７细胞中有效表达ＧＭ－ＣＳＦ；小鼠骨骼肌直接注射ｐＳＶＬ／ＧＭ－ＣＳＦ（１００μｇ／及２００μｇ），在血清中可检出ＧＭ－ＣＳＦ，在１０～１５ｄ达高峰（８Ｕ／ｍｌ），为临床应用提供了实验基础；导入ＧＭ－ＣＳＦ基因的Ｋ５６２细胞可表达ＧＭ－ＣＳＦ，且经ＧＭ－ＣＳＦ基因修饰的Ｋ５６２细胞增殖状态明显优于对照组，提示对造血细胞肿瘤单用ＧＭ－ＣＳＦ应慎重，而与其它细胞因子联合应用可能是一个合理的方案。