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61.
地铁隧道盾构施工参数对地表沉降影响的试验研究 总被引:2,自引:0,他引:2
以南京地铁2号线某区间隧道为背景,研究了盾构法施工中的盾构施工参数(包括推进力、工作面土压力、刀盘扭矩等)对地表沉降的影响,通过对现场监测结果的分析,总结了地表沉降规律,对后续工程施工具有指导意义。 相似文献
62.
高速铁路的发展对民航的冲击及反思 总被引:1,自引:0,他引:1
在分析我国铁路高速化发展趋势及特点的基础上,研究了高铁发展对民航运输业、航空公司及航空消费群体3个方面的冲击与影响,并进一步探讨了民航的应对策略,从而给我国高铁的健康发展以有益的启示. 相似文献
63.
Si Lüsheng 《西安交通大学学报(英文版)》2006,18(1):44-48,77
Objective To construct eukaryutic expression vector of HPV18 L1- E6, E7 chimeric gene and examine the humorul and cellular immune responses induced by this DNA vaccines in mice. Methods The C-terminal of major rapsid protein L1 gene and mutant zinc finger domains of early E6/7 oncogenes in HPV18 were integrated and inserted into eukaryotic expression vector pVAX1 to generate vaccines pVAX1-L1E6Mxx, ETMxx. CHO cells were transiently transfected with the individual construct. Target protein expressions in the lysate of the transfected cells were measured by ELISA and immunocytochemistry After BALB/c mice were vaccinated with various recombinant plusmids(pVAX1- L1-E6M3 or pVAX1-L1-E7M3 ) and immunie adjuvants (pLXHDmB7-2 or LTB) through different administration routes (intramuscular or intranasal) , the great cellular immune responses were produced us revealed by delayed-type hypersensitivity (DTH) and lymphocyte proliferation, and the expression of IL-4 and IFN- 7 cells in CD4^+ and CD8^+ subpopulations. Results The highly efficient expression of pVAX1-L1E6Mxx, E7Mxx vector in host eukaryotic cells were demonstrated both by ELISA and immunocytochemistry. The level of specific serum IgG against HPV in experiment groups mice was much higher than that of control group, and intranuscular immunization group had the highest antibody level. Intramuscular immunization groups were superior to intranasal immunization groups in DTH response, splenocyte proliferation and CD8^+ IFN-γ^+ cells number, but CD4^+ IL4^+ cell number was higher in intranasul immunization groups. The immunization groups using pLXHDmB7.-2 as adjuvant were superior to other groups in immunorespouse. Conclusion These DNA vaccines produce remarkable cellular and humorul immune responses in the mouse and may provide us prophylatic and therapeutic candidates for HPV induced cancer treatment. 相似文献
64.
重庆云阳长江大桥设计要求必须采用后支点式挂篮,该挂篮具有承载能力大、变形小、受力明确、造价低等特点。斜拉桥用后支点式挂篮进行主梁悬浇施工,这在国内斜拉桥悬浇施工中十分少见,具有一定的设计难度。 相似文献
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用~3H和地高辛配基标记肿瘤坏死因子-αcDNA探针以及原位杂文技术检测33例子宫颈癌和28例阴道尖锐湿疣活检标本中的TNF mRNA。结果表明,两种标记探针原位杂交结果基本一致,~3H标记探针敏感性稍高于地高辛配基标记探针。但放射性核素探针的固有缺点及非同位素探针的安全、快速、方便使非同位素探针有着日益广泛的应用前景。 相似文献
67.
应用免疫组织化学方法评价组织芯片的可靠性 总被引:2,自引:0,他引:2
目的探讨组织芯片技术在免疫组化试验中的可靠性和提高组织芯片可信性的方法。方法选择82例人乳腺癌标本石蜡蜡块,利用传统制片技术和自行研制的专用器具分别制作常规石蜡切片和组织芯片,采用免疫组化技术检测雌激素受体(ER)、孕激素受体(PR)的表达。结果采用自行研制的专用器具制备组织芯片,所得样本的可分析率均在92.9%以上,且随着对同一标本取材数量的增加,其可分析标本率也明显上升。采用传统方法检测的82例乳腺癌标本中ER和PR的阳性率分别为61%和58.5%;采用组织芯片技术,一式一份取材,其阳性率分别为51.9%和50.0%,一式两份方式取材,其阳性率分别为53.8%和53.8%,采用一式三份方式取材,其阳性率分别为57.1%和60.7%,且三种不同取材方式的检测结果与传统制片法之间并无统计学差异(P>0.75)。结论在采用统一制备标准并保证组织芯片质量的前提下,挖取直径1.5mm的组织样本制备组织芯片,尽管组织芯片技术与传统方法检测的结果的一致率仍随着对同一标本取材次数的增加而提高,但是三种不同取材方式的检测结果之间并无统计学差异。因此,一式一份取材制备组织芯片完全可替代传统制片技术用于免疫组织化学乃至原位杂交、荧光原位杂交技术的研究,且可保证组织芯片的可信性和高通量特征。 相似文献
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69.
Abstract Many equilibrium models and algorithms based on homogeneous motorized traffic have been devised to model urban transport systems in developed countries, but they are inadequate when it comes to represent mixed-traffic urban transport systems, including automobiles, transit, bicycles, and pedestrians, in developing countries such as China or India. In these cases, traffic flow on a road segment is an aggregated result of travellers' combined mode/route choices and corresponding interactions. Therefore, a special assignment model and algorithm are needed for modeling these distinct behaviors. In this article, the structure of a mixed-traffic urban transport system is analyzed and then expanded and represented using a hierarchical network model based on graph theory. Based on the analysis of travelers' combined mode/route choices, generalized travel cost functions and link impedance functions for different modes are formulated, where the interferences between different modes on the same road segments are taken into account. Due to the ‘asymmetric’ nature of these functions, a variational inequality model is proposed to represent the equilibrium assignment problem in a mixed-traffic urban transport system. The corresponding solution algorithm is also presented. Finally, a numerical example is provided to illustrate the practicality of the proposed model and algorithm. 相似文献
70.
Abstract Dial's algorithm is one of the most effective and popular procedures for a logit-type stochastic traffic assignment, as it does not require path enumeration over a network. However, a fundamental problem associated with the algorithm is its simple definition of ‘efficient paths’, which sometimes produces unrealistic flow patterns. In this paper, an improved algorithm based on the route extension coefficient is proposed in order to circumvent this problem, in which ‘efficient paths’ simultaneously consider link travel cost and minimum travel cost. Path enumeration is still not required and a similar computing efficiency with the original algorithm is guaranteed. A limitation of the algorithm is that it can only be applied to a directed acyclic network because a topological sorting algorithm is used to decide the order of the sequential calculation. A numerical example based on the Beijing subway network illustrates the effectiveness of the proposed algorithm. It is found that it is able to exclude most unrealistic paths, but include all reasonable paths when compared with path enumeration and the original Dial's algorithm. 相似文献