Direct resolution of the pitch of DNA on positively charged lipid bilayers by frequency-modulation AFM

High resolution structural studies of DNA and DNA binding proteins by atomic force microscopy (AFM) require well-bound samples on suitably flat substrates. Adsorbing the DNA onto a positively charged supported lipid bilayer has previously been shown to be a potentially effective strategy for structural studies with AFM. Here, using our home-built frequency-modulation AFM (FM-AFM), we show that these bilayer substrates are only maximally effective for high resolution AFM when the samples are short, linear DNA, compared with circular plasmid DNA. We find that, with the former sample, the measured width of the DNA is about 2 nm, the known DNA diameter, and there is a clear height modulation along the length of the DNA with a periodicity of about 3.4 nm, in excellent agreement with the known pitch of the double helix. This sample preparation strategy is expected to enable higher resolution studies of DNA and DNA binding proteins with FM-AFM than that can presently be achieved.     

T_H1 AND T_H2 CELL ANTIGEN RECEPTORS IN EXPERIMENTAL LEISHMANIASIS

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LEISHMANIA PROMASTIGOTES EVADE INTERLEUKIN 12 INDUCTION BY MACROPHAGES AND STIMULATE A BROAD RANGE OF CYTOKINES FROM CD_（4）~（＋

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SD大鼠肝脏及附属管道的应用解剖

通过对150只成年SD大鼠肝脏及其附属管道的解剖学研究，明确了大鼠肝脏的位置、分叶、形态、韧带、管道系统及与周围脏器的毗邻关系，同时测量了肝脏各叶径线、重量百分比及肝脏附属管道的长度和直径。结果表明：大鼠肝脏分为6叶，即左外叶、左内叶、中叶、右叶、尾状叶、乳头叶，各叶县有相对独立的Glisson系统和肝静脉回流系统；各肝叶形态、比例较为恒定。本文还对以大鼠作肝胆疾病医学研究模型，如肝叶切除、肝脏再生、肝移植等的应用解剖问题进行了讨论。     

Roll Dynamics of Commercial Vehicles

An analytical model is developed here for studying the roll dynamics of commercial vehicles. Large displacements and rotations are accounted for in this nonlinear model so that it can be used for the study of roll dynamics well beyond the limits of wheel lift-off. The model is used to illustrate some of the dynamic phenomena in vehicle rollover, especially the interactive coupling between the roll and the vertical modes of motion. The influence of suspension backlash on rollover resistance is demonstrated, and the phenomenon of roll motion resonance is illustrated to suggest new means for evaluating vehicle rollover sensitivity.     

Hub location with flow economies of scale

A characteristic feature of hub and spoke networks is the bundling of flows on the interhub links. This agglomeration of flows leads to reduced travel costs across the interhub links. Current models of hub location do not adequately model the scale economies of flow that accrue due to the agglomeration of flows. This paper shows that current hub location models, by assuming flow-independent costs, not only miscalculate total network cost, but may also erroneously select optimal hub locations and allocations. The model presented in this paper more explicitly models the scale economies that are generated on the interhub links and in doing so provides a more reliable model representation of the reality of hub and spoke networks.     

汽车筒式液阻减振器技术的发展

分析了汽车乘坐舒适性／行驶平顺性和操作稳定性对筒式液阻减振器特性的要求，提出汽车在不同行驶工况减振器特性的要求是不的；分析了被动式减振器的发展历程及非充气和充气减振器的特点，阐述了机械控制式可调阻尼减振器，电子控制式减振器以及电流变和磁流变液体减器等的结构特点，工作原理及其动态特性；分析了筒式液阻减振器其于经验设计／实验修正开发方法的缺点，阐述了基于CAD／CAE技术的现代设计开发方法的过程及其关键问题，最后分析了我国筒式液阻减振器技术的发展状况及问题，展望了减振器技术的发展前景。     

Evaluation of forced flows on freeways with single‐loop detectors

This paper documents the development of a simple method for identifying and/or predicting freeway congestion using single loop detection systems. The proposed algorithm is simple and easy to incorporate into most freeway management systems. The Washington State Department of Transportation's Traffic Systems Management Center (TSMC) sponsored the original study. The investigation also led to a recommendation to replace the original TSMC definition of congestion or forced flow conditions with a more reliable indicator. Although, the TSMC has recently implemented a more advanced prediction system based on fuzzy set theory and neural networks to further identify patterns and rules for ramp metering strategies, the findings presented here continue to be constructive to freeway managers looking for quick and easy analyses that rely solely on single‐loop detection systems. The Seattle Area freeway study section used for the original study was the portion of mainline 1–5 northbound starting at the downtown Seattle Station 108 and ending at the Mountlake Terrace Station 193. Several days' worth of volume and lane‐occupancy data were collected for the afternoon time period from 2:30 p.m. to 6:30 p.m. Time intervals of 20 seconds were chosen for each data collection period. Important products of this research include the following:

• simple, and more reliable criterion for the definition of “bottleneck” or forced flow conditions than that originally used by the TSMC.
• simple, and reliable criterion for predicting impending “bottlenecks” or forced flow conditions.
• A proposed variable for improved selection of the appropriate metering rate. (Further analysis of the use of this variable for determining metering rates is recommended for future studies.

The proposed criteria are simple and easy to incorporate into current freeway management computer systems. Further investigation of freeway performance measurement using volume and occupancy data obtained from single‐loop systems is currently being performed.     

The design of bus route systems — An interactive location-allocation approach

An interactive modelling approach is developed to solve the practical problem of bus route network design. Possible bus routes are identified with facilities which can be located. Zones or pairs of zones in the urban area are identified with customers who will be allocated to the established facilities. It is shown that the classical Set Covering Problem is useful under the assumption of fixed demand; the Simple Plant Location Problem is effective under the assumption of demand which is sensitive to the level of bus service provided.     

Conformity: How VMT-speed distributions can affect mobile emission inventories

Transportation conformity is a US regulatory process that requires that transportation modeling be integrated with air quality modeling. Consequently, every change to either modeling process is undertaken with great scrutiny by the regional governments, who have to use the models for demonstrating conformity. This paper explores the "trip versus link debate," which stems from the fact that the standard travel demand models used by most metropolitan planning organizations are primarily link oriented, while the air quality models have been primarily trip oriented. Using the Sacramento region we examine the effects on mobile source emissions inventories when speed-VMT distributions are constructed using the trip and link-based philosophies. The results of our study indicate that trip-based VMT-speed distributions produce consistently lower emissions estimates than the link-based distributions. We use the results to assert that deciding between a trip-based or link-based conformity modeling process involves more than the technical difficulty of changesto the models or the potential political ramifications, it involves assessing which method will provide the most accurate estimates of regional motor vehicle emissions. We also examine ways to think about constructing mobile source emission inventories.