20世纪美国应对机动性的交通构想及高速公路发展历程

为了阐述20世纪除汽车机动性外,交通发展经历的最重要的进程——城市高速公路的出现,考察了过去100年来美国城市交通规划的历史。通过回顾各种研究资料,认为资助州际公路发展所采用的方法使联邦和州公路工程师成为主管,这影响了公路的选线及设计。联邦公路工程师只关注机动车通过效率的最大化,而在很大程度上忽略了城市的其他利害关系。没有先进的规划理念指导、过度建设、稀疏、放射环状的道路网对于加强机动性取得了极大的效益,但通常要为此付出巨大的社会和环境成本。最后指出,在实践中要重新审视具有百年历史的协调交通与土地利用规划的观点,而且,公共财政权宜之计式的政治决策方式将对工程、出行和城市形态产生深远的影响。·     

The exclusive economic zone: criteria and machinery for the resolution of international conflicts between different users of the EEZ

It is highly probable that one of the results of the Third United Nations Conference on the Law of the Sea will be the recognition of a new jurisdictional zone—the exclusive economic zone (EEZ). This 200-mile zone will be used for a variety of potentially conflicting purposes by both the coastal state and non-coastal states and it is therefore important to ensure that the new convention emerging from the conference should establish criteria and make provision for machinery for the resolution of the international conflicts between different users which will undoubtedly arise. This paper contains a critical analysis of the latest draft articles under consideration at the Conference and considers the criteria by reference to which disputes might be resolved.     

Training Needs of Coastal Resources Decision Makers in Ohio's Lake Erie Basin

This article describes results from a study focused on informational and knowledge needs of local coastal resources decision makers in the Ohio Lake Erie basin. New information was obtained through a series of focus groups of local decision makers and key training providers in the basin. The results suggest that training providers and local coastal resources decision makers have differing perceptions of knowledge and information needs and training venues. The results suggest a role for state and federal agency training providers as coordinators and facilitators of an enhanced learning network among decision makers, including support of an information clearinghouse, adoption of appropriate e-based learning modalities, and regionally based learning and practice networks to improve the function of the training market to meet local decision maker needs.     

黎明的喧嚣——历史时期过渡的模式

通常历史学家用生日、死亡、政治大事或单一的文化事件等突然的标记来为历史分期,这使历史时间发生断裂,时代错误成为尴尬的问题.本文认为,时期的划分不应将鲜活的时间隔断.不同时期应连接流动的时间里相互联系的事件.基于赫尔德的比喻,作者提出将黎明的喧嚣作为一个历史时期过渡到下一个历史时期的模式.本文旨在从"时期"这一概念的词源、音乐及诗歌对黎明的喧嚣这一模式的再现等角度重新思考分期化问题,以说明多样性是历史时期过渡的界定性特征.时期点缀时间的流动,崭新重叠的组合并不为了真理而竞争,而是为了提供互补的含义.     

Effects of land-use transport scenarios on travel patterns: a multi-state supernetwork application

Understanding travellers’ response is essential to address policy questions arising from spatial and transport planning sectors. This paper demonstrates the usefulness of the multi-state supernetwork approach to investigate the effects of land-use transport scenarios on individuals’ travel patterns. In particular, it illustrates that multi-state supernetworks are capable of representing activity-travel patterns at a high level of detail, including the choice of mode, route, parking and activity location. Multi-faceted activity-travel preferences can be accommodated in supernetworks. Using a micro-simulation approach, the adaptation of individuals’ travel patterns to policies can be readily captured. The illustration concerns hypothetical land-use and transport scenarios for the city of Rotterdam (The Netherlands), focusing on accessibility changes, modal substitution and shift in the use of transport and location facilities.     

The continental shelf and the exclusive economic zone: the problem of delimitation at UNCLOS III

If the Third United Nations Conference on the Law of the Sea succeeds in adopting a new Caracas Convention on the Law of the Sea, it is likely to embody detailed provisions on the delimitation of both the continental shelf and the new zone, the exclusive economic zone. In this paper, the most recent draft of these rules—contained in the Revised Single Negotiating Text—is subjected to a detailed and critical scrutiny which leads to the conclusion that the Sixth Session of the Conference still has a great deal of work to do before agreement can be reached on a satisfactory set of rules.     

Maritime commercial malpractices and piracy under international law

The term 'Piracy' tends to be used in a loose, popular sense to refer to various acts of violence or lawlessness at sea which are not, strictly speaking, acts of piracy in law. In this article, the author examines the concept of piracy under international law and related notions such as hijacking and mutiny. In this context, he shows that the 'malpractices' of robber bands in such places as the inshore waters of Nigeria and The Philippines are not acts of piracy jure gentium.     

猴细小病毒Vp2的克隆、表达和鉴定

目的　克隆、表达和鉴定猴细小病毒 (simianparvovirus,SPV)Vp2蛋白。方法　用设计的SPVVp2区的特异性引物 ,PCR法扩增SPVVp2基因DNA片断 ;将获得的基因片断插入 pThioHisA载体中 ,转化大肠杆菌DH5α后挑选阳性克隆 ,利用限制性内切酶酶切和核酸序列分析技术鉴定重组质粒 ;以LB培养液培养转化有插入SPVVp2基因的pThioHisA载体的大肠杆菌 ,以终浓度 1mmol·L -1的IPTG诱导蛋白的表达 ;用SDA PAGE和Westernblot分析和鉴定表达的蛋白。结果　经限制性内切酶酶切和核酸序列分析 ,获得了插入pThioHisA载体框架的SPVVp2基因的表达质粒 ;阳性转化大肠杆菌在IPTG诱导下获得了SPVVp2蛋白的表达 ;SDA PAGE和用抗 Thio及抗 SPVVp2的特异性抗体的Westernblot分析证实了表达蛋白的特异性。结论　获得了SPVVp2基因的表达质粒并在大肠杆菌中得了高效表达 ,为进一步建立SPV感染的检测方法和研究SPV的血清流行病学等奠定了基础。     

The Development of a Visualization Methodology for Integrated Coastal Management

Coastal management information is frequently communicated to stakeholders and the public through complicated management documents and engineering plans. With the recognition that public involvement in coastal decision-making processes should be widened have come calls to develop new techniques to communicate complicated coastal information. Using Virtual Reality Geographical Information Systems and visualization packages, such information may be presented using formats more suitable for public consultation and information dissemination exercises than those currently employed. Using a site on the north Norfolk coast of England, an integrated Geographical Information Systems based methodology is presented that allows the visualization of proposed coastal management interventions. Visualizations have been produced that can be published in traditional paper-based management documents, or electronically. The different visualizations are compared and the technical issues surrounding their use discussed. It is argued that the methodology has clear advantages over traditional communication methods, although further research is necessary to determine how it may be practically employed by coastal managers.