Novel experimental strategy for high resolution AFM imaging of membrane-associated bacterial toxins

Bacterial pore-forming toxins （PFTs） are essential virulence factors of many human pathogens. Knowl- edge of their structure within the membrane is critical for an understanding of their function in pathogenesis and for the development of useful therapy. Atomic force microscopy （AFM） has often been employed to structurally interrogate many membrane proteins, including PFTs, owing to its ability to produce sub-nanometer resolution images of samples under aqueous solution. However, an absolute prerequisite for AFM studies is that the samples are single-layered and closely-packed, which is frequently challenging with PFTs. Here, using the prototypical member of the cholesterol-dependent cytolysin family of PFTs, perfringolysin O （PFO）, as a test sample, we have developed a simple, highly robust method that routinely produces clean, closely-packed samples across the entire specimen surface. In this approach, we first use a small Teflon well to prepare the supported lipid bilayer, remove the sample from the well, and then directly apply the proteins to the bilayer. For reasons that are not clear, bilayer preparation in the Teflon well is essential. We anticipate that this simple method will prove widely useful for the preparation of similar samples, and thereby enable AFM imaging of the greatest range of bacterial PFTs to the highest possible resolution.     

基于GOOSE机制的城市轨道交通中压环网继电保护方案优化

针对目前城市轨道交通中压环网继电保护存在的问题,提出了基于GOOSE(面向通用对象的变电站事件)机制的中压环网的继电保护优化方案,有效地解决了传统过流保护级差配合困难的问题.该方案是以GOOSE光纤以太网为基础,以闭锁逻辑为核心的数字通信电流保护方案.将GOOSE信号进行分类,并对中压供电环网系统各种可能出现的故障进行动作行为分析.最后,通过RTDS(动态数字仿真系统)仿真试验,证明该方案在各种故障情况下,都能保证继电保护的选择性、速动性、灵敏性和可靠性.     

Robust traffic assignment model: Formulation,solution algorithms and empirical application

ABSTRACT

The deterministic traffic assignment problem based on Wardrop's first criterion of traffic network utilization has been widely studied in the literature. However, the assumption of deterministic travel times in these models is restrictive, given the large degree of uncertainty prevalent in urban transportation networks. In this context, this paper proposes a robust traffic assignment model that generalizes Wardrop's principle of traffic network equilibrium to networks with stochastic and correlated link travel times and incorporates the aversion of commuters to unreliable routes.

The user response to travel time uncertainty is modeled using the robust cost (RC) measure (defined as a weighted combination of the mean and standard deviation of path travel time) and the corresponding robust user equilibrium (UE) conditions are defined. The robust traffic assignment problem (RTAP) is subsequently formulated as a Variational Inequality problem. To solve the RTAP, a Gradient Projection algorithm is proposed, which involves solving a series of minimum RC path sub-problems that are theoretically and practically harder than deterministic shortest path problems. In addition, an origin-based heuristic is proposed to enhance computational performance on large networks. Numerical experiments examine the computational performance and convergence characteristics of the exact algorithm and establish the accuracy and efficiency of the origin-based heuristic on various real-world networks. Finally, the proposed RTA model is applied to the Chennai road network using empirical data, and its benefits as a normative benchmark are quantified through comparisons against the standard UE and System Optimum (SO) models.     

Nanoparticle emission characteristics and reduction strategies by boost pressure control and injection strategies in a passenger diesel engine

This research attempted to analyze nanoparticles and other harmful exhaust emissions in accordance with injection strategies and air-fuel ratio (AFR) changes for small diesel engines. The emission characteristics were analyzed in the medium-speed condition, which is the main driving range of a diesel engine. In the case of particulate matter (PM), the number of particles was measured, analyzed, and compared to identify the correlation and emission characteristics of nanoparticles by using a dilution device and condensation particle counter (CPC), which are international standards for particle measurement recommended by the Particulate Measurement Programme (PMP). The engine torque tended to be reduced as pilot injections were added, and the torque was increased by the increased boost pressure, but reduced by the exhaust pressure increase in a part of the low-load range. The number of nanoparticles was not influenced greatly by the change in AFR, but the reduction effect on the PM weight was great depending on the boost pressure increase. In addition, the number of nanoparticles tended to increase as the fuel injection timing became closer to TDC in all conditions, and its difference became larger with an increase in AFR. In addition, in the case of the pilot injection, nanoparticle emission showed similar characteristics depending on the main injection timing, but it was increased by advanced injection timing when performing the main injection only, and the number of the nanoparticles increased as pilot injections were added. Last, the optimal conditions for EMS calibration were analyzed by selecting the conditions of torque reduction and NOx increase within 5 % from all of the engine operating conditions; optimized conditions are presented.     

Vibration reduction of H/Shaft using an electromagnetic damper with mode change

The objective of this study is to develop a damper that can reduce the amplitude of vibration in various frequency ranges. Previous H/Shaft vibration reduction methods work in a passive way. A dynamic damper reduces the amplitude of vibration at its first mode, but vibration still appears at the second mode. A mass damper or hollow shaft can shift the natural frequency to a lower or higher region. The fixed operating frequency prevents vibration from being reduced outside the operating frequency range. The proposed damper uses electromagnets as either masses or actuators to change the damper mode between dynamic damper mode and mass damper mode. The electromagnetic damper (EMD) can change its mode to respond to the vibration excitation at both low and high frequencies. The vibration reduction performance was evaluated by FRF tests in laboratory and vehicle conditions. The results were compared with those of a dynamic damper and indicate that the amplitude of vibration is reduced by 95.6 % when the EMD is implemented on an H/Shaft, whereas only 61.9 % vibration reduction is achieved by the dynamic damper.     

Modelling,simulation and applications of longitudinal train dynamics

ABSTRACT

Significant developments in longitudinal train simulation and an overview of the approaches to train models and modelling vehicle force inputs are firstly presented. The most important modelling task, that of the wagon connection, consisting of energy absorption devices such as draft gears and buffers, draw gear stiffness, coupler slack and structural stiffness is then presented. Detailed attention is given to the modelling approaches for friction wedge damped and polymer draft gears. A significant issue in longitudinal train dynamics is the modelling and calculation of the input forces – the co-dimensional problem. The need to push traction performances higher has led to research and improvement in the accuracy of traction modelling which is discussed. A co-simulation method that combines longitudinal train simulation, locomotive traction control and locomotive vehicle dynamics is presented. The modelling of other forces, braking propulsion resistance, curve drag and grade forces are also discussed. As extensions to conventional longitudinal train dynamics, lateral forces and coupler impacts are examined in regards to interaction with wagon lateral and vertical dynamics. Various applications of longitudinal train dynamics are then presented. As an alternative to the tradition single wagon mass approach to longitudinal train dynamics, an example incorporating fully detailed wagon dynamics is presented for a crash analysis problem. Further applications of starting traction, air braking, distributed power, energy analysis and tippler operation are also presented.     

A study on the stability of a motorcycle wheel–swingarm suspension with chain transmission

The present study describes a possible driving mechanism for a self-excited oscillation observed in motorcycle dynamics, often referred to as chatter. This phenomenon, affecting the performance of road racing motorcycles, has been simulated in straight running braking manoeuvres with multibody motorcycle models. It involves rear suspension bounce and driveline oscillation in the frequency range between 17 and 22?Hz. A simplified model of a motorcycle rear suspension with chain transmission is proposed and its stability in equilibrium configurations is studied via eigenvalue analysis. The sensitivity with respect to all its governing parameters is analysed by means of stability maps and the self-excitation mechanism is explained with the aid of energy balance analysis and phase diagrams. It is found that the key role for the instability onset is played by the gradient of the nonlinear characteristic slip function of the tyre.     

A receding horizon sliding control approach for electric powertrains with backlash and flexible half-shafts

Active control of electric powertrains is challenging, due to the fact that backlash and structural flexibility in transmission components can cause severe performance degradation or even instability of the control system. Furthermore, high impact forces in transmissions reduce driving comfort and possibly lead to damage of the mechanical elements in contact. In this paper, a nonlinear electric powertrain is modelled as a piecewise affine (PWA) system. The novel receding horizon sliding control (RHSC) idea is extended to constrained PWA systems and utilised to systematically address the active control problem for electric powertrains. Simulations are conducted in Matlab/Simulink in conjunction with the high fidelity Carsim software. RHSC shows superior jerk suppression and target wheel speed tracking performance as well as reduced computational cost over classical model predictive control (MPC). This indicates the newly proposed RHSC is an effective method to address the active control problem for electric powertrains.     

Insights into vehicle trajectories at the handling limits: analysing open data from race car drivers

Race car drivers can offer insights into vehicle control during extreme manoeuvres; however, little data from race teams is publicly available for analysis. The Revs Program at Stanford has built a collection of vehicle dynamics data acquired from vintage race cars during live racing events with the intent of making this database publicly available for future analysis. This paper discusses the data acquisition, post-processing, and storage methods used to generate the database. An analysis of available data quantifies the repeatability of professional race car driver performance by examining the statistical dispersion of their driven paths. Certain map features, such as sections with high path curvature, consistently corresponded to local minima in path dispersion, quantifying the qualitative concept that drivers anchor their racing lines at specific locations around the track. A case study explores how two professional drivers employ distinct driving styles to achieve similar lap times, supporting the idea that driving at the limits allows a family of solutions in terms of paths and speed that can be adapted based on specific spatial, temporal, or other constraints and objectives.