The role of interleukin-8 produced by tumor induced fibroblasts in the development of cutaneous melanoma

Objective To determine the role of interleukin-8 (IL-8) produced by tumor induced fibroblasts in the development of cutaneous melanoma. Methods B16 melanoma cells induced L929 fibroblasts phenotype was transdifferentiated to myofibroblasts (MF) by co-culture in vitro. MF was monitored by morphology and immunophenotype for a-SMA. The level of IL-8 was detected by ELISA. The effect on B16 cell proliferation rate was estimated using MIT method in vitro. Melanoma implanting model was constructed in C57 mice. Results L929 MF phenotype could be modulated by B16 melanoma cells-derived transforming growth factor-β1 (TGF-β1) and elevated the levels of IL-8. L929 MF did not influence the B16 melanoma cells viability in vitro, but shortened the time of tumor formation and increased the incidence rates of tumors in C57 implanting model mice. Conclusion Fibroblasts can be activated by tumor cells and produce IL-8, which acts as an inflammatory cytokine promoting the development of cutaneous melanoma.     

The changes of neuronal activity of the zone incerta in a rodent model of Parkinson＇s disease

Objective To study the changes in neuronal activity of the zone incerta (ZI) following the unilateral lesion of the nigrostriatal pathway. Methods Eiectrophysiological recordings of ZI neurons were made in normal rats and in two groups of rats at different time intervals after injection of 6-hydrodopamine (6-OHDA) into the pars compacta of snbstantia nigra by extracellular recording in vivo. Results The results showed a significant increase in the mean firing rate of ZI during the second and fourth weeks after 6-OHDA lesion [-n=32, (3.6±2. 2)Hz, P<0.001; n=35, (9.3±6.6)Hz, P<0. 001, respectively] compared to that of normal rats [n=39, (9.2±5.2)Hz]. However, no significant change was observed between two groups of 6-OHDA-lesioned rats. With regard to firing pattern, 7.7%(3/39) of ZI neurons discharged regularly, 82.1% (32/39) irregularly and 10. 3% (4/39) in bursts in normal rats. During the second week after 6-OHDA lesion, the regular, irregular and bursting firing neurons recorded in the ZI were 9.4% (3/32), 59. 4% (19/32) and 31.3% (10/32), and during fourth week, the regular, irregular and bursting firing neurons were 14.3% (5/35), 57.1% (20/35) and 28. 6% (10/35), respectively. The firing pattern of the neurons in the three different groups did not change significantly. Conclusion These results suggest that the firing rate of ZI neurons in 6-OHDA-lesioned rats is increased significantly, which may contribute to the pathophysiology of Parkinson's disease.     

Effect of ABCA1-V771M polymorphism on plasma lipid levels and its relationship with coronary atherosclerotic heart disease

Objective To explore the risk association of ABCA1-V771M polymorphism with coronary heart disease (CHD) in Hart nationality in Northwest of China. Methods With case-control study, ABCA1-V771M polymorphism was detected in 204 unrelated Hart nationality people in Northwest of China, and all the subjects by coronary angiography were grouped into 106 cases and 98 controls. The genotypes and alleles frequency distribution of ABCA1-V771M polymorphisms were analyzed by PCR-RFLP analysis, and the clinical statistics of serum lipids were compared and its effects of ABCA1-V771M polymorphism on the plasma lipid levels and coronary atherosclerotic heart disease were analyzed. Results The genotypic frequencies of ABCA1-V771M polymorphism matched well under Hardy-Weinberg equilibrium (P>0.05), V and M allelic frequencies were 33.3% and 66.7%. In comparison with VV VM genotype carriers, MM genotypes carriers had much lower plasma levels of HDL-C (P<0. 001) and much higher plasma levels of TG (P<0. 05). M allelic frequency in CHD group was significantly higher than V allelic frequency (P<0. 05). M allele was related with more severity of atherosclerosis in the coronary artery than V allele (P<0.05). However, there was no obvious difference in the incidence of AMI among carriers with three genotypes of ABCA1-V771M polymorphism (P>0.05). Conclusion ABCA1-V771M polymorphism was not only associated with the plasma levels of HDL-C and TG, but also related to the susceptibility and severity of coronary atheroselerotic heart disease. Moreover, M771 allele appeared to be atherogenie among Han population in Northwest of China.     

Study on T lymphocyte subsets and NK cells in patients with Graves＇ disease combined with type 2 diabetes

Objective To investigate changes in T lymphocyte subsets and NK cells in patients with simple Graves' disease (GD)and Graves' disease combined with type 2 diabetes mellitus (GD/T2DM). Methods Fifteen cases of GD/ T2DM were selected from our hospital from November 2001 to November 2004. Before and after therapy thyroid function, thyroglobulin antibody (TGA), thyroid microsomal antibody (TMA) and blood glucose level were measured, and T lymphocyte subsets (CD3, CD4, CD8, CD4/CD8) and NK cells (CD56) were measured by immunofluorescence double labeling monoclonal antibody and flow cytometry, respectively. At the same time, comparison was made with simple GD (15 cases), T2DM (15 cases) and healthy control (20 cases). Results Before therapy, CD4/CD8, CD4 and NK cells in GD/T2DM were less than normal, and there was no significant difference in comparison with simple GD (P<0.05). In T2DM group, only CD4/CD8 and CD4 were less than those of healthy controls (P<0.05). When thyroid function recovered after 1 to 3 months of methimazole treatment in both GD/T2DM and simple GD groups, various indexes recovered, which were more obvious in simple GD. Conclusion Immune hypofunction of GD may be the key to the immune abnormality of GD/T2DM, which is more significant than that of simple GD or T2DM. The recovery of thyroid function and immune abnormality is not consistent, and the recovery of GD is more significant than that of GD/T2DM.     

探讨沥青路面局部渗水的原因及处理方法

通过武汉市汉蔡高速公路沥青路面出现的局部渗水现象,分析沥青路面产生局部渗水的原因及处理的必要性,提出对沥青路面局部渗水的处理原则及处理方法。     

南京长江隧道盾构始发井深基坑降水方案设计

南京长江隧道工程盾构始发井深大基坑地质条件复杂,地下水位高,并与长江水力联系密切,降水难度大.文章结合实践,重点阐述了本工程采用深井管井与轻型井点结合降水方案的设计,其中包括涌水量的计算、降水井数量的确定及降水井间距布设等参数研究,对今后同类工程的修建有一定的借鉴意义.     

拉力型锚索锚固段长度的一种确定方法

文章分析了锚杆拉拔试验的结果,提出了非全长粘结型锚索锚固段剪应力沿长度分布的二次抛物线模式,以及确定锚固段长度的合理方法,并以工程实例说明了文中提出的锚索锚固段长度计算方法的合理性.     

基于船舶阻力计算方法确定浮泥的适航密度值

效仿通过船模阻力试验来界定浮泥适航密度值的方法,提出采用船舶阻力计算方法确定浮泥的适航密度值。基于求解NS方程的CFD方法,建立了模拟浮泥中船舶运动流场的数学模型,模型中浮泥的本构关系用改进的Herschel-Bulkley模型来描述。以连云港航道现场泥样和超大型油船KVLCC2为例,采用所建立的数学模型计算了船舶以不同速度在不同密度浮泥中航行受到的阻力。通过数学手段分析了船舶阻力随浮泥密度变化的趋势,确定该港口航道浮泥的适航密度值为1 210 kg/m3,该值与采用流变试验方法和船模阻力试验方法得到的适航密度值接近,证明了文章提出方法的有效性。     

准动态分析法在工程船舶锚泊定位系统设计中的应用研究

工程船舶在海上作业时都需配备定位系统,如何配置安全可靠、满足作业要求和具有市场竞争力的锚泊定位系统已成为探讨热点。通过对现行锚泊定位系统分析的几种基本方法的理论特点及应用特性比较,确定应用准动态方法对工程船进行锚泊定位分析的可行性,并阐述了准动态分析方法的基本理论及特点,与全动态分析方法进行了理论对比分析,从而进一步体现了准动态方法应用于工程船舶锚泊定位的合理性。以某工程船为例,分别应用准动态分析软件ARAINE及全动态分析软件DEEPC进行锚泊定位计算对比分析,论证了应用准动态方法分析工程作业船舶锚泊定位的优势及特点。从理论分析及实际应用角度充分证明了,应用准动态方法对工程船进行锚泊定位分析既保证工程精度要求,又可显著提高计算效率,具有明显优势。     

基于CFD模拟的船舶空调舱室热舒适性研究

针对船舶舱室热环境的特殊性,建立了适用的CFD计算模型,并对其进行了实验验证。在该模型的数值模拟结果基础上,以PMV-PPD热舒适性评价指标和DNV COMF-C(3)规范为依据,对船舶舱室空调气流组织进行优化。优化结果表明,相比于优化前,优化后的餐厅和会议室的热舒适性得到改善,热环境满意人数分别增加了52.7%和28.9%。结合CFD模拟和热舒适性评价分析的优化方法,在节约时间和实验成本的同时,为船舶空调系统的设计和优化提供了重要依据。