Role of candesartan against cerebral ischemia and oxidative damage in normotensive rats

Objective Angiotensin Ⅱ (Ang Ⅱ ) contributes to modulating blood pressure by stimulation of Ang Ⅱ AT1 receptors. We devised a rat transient middle cerebral artery occlusion (MCAO) model to assess whether oxidative damage is decreased after pretreatment with Angiotensin Ⅱ AT1 receptor blocker (ARB). Methods After 2 weeks pretreatment with ARB 0. 5 and 1 mg/kg, the male Wister rats were subjected to 2 h middle cerebral artery occlusion (MCAO). At 24 h, the lumen diameter of middle cerebral artery, the plasma level of 8-hydroxy-2'-deoxyguanosine (8-OHdG), and HIF-1 a levels were recorded and compared. Results After pretrcatment with ARB 0.5 and 1 mg/kg, blood pressure did not significantly change compared with that of controls. In the group of candesartan at 1 mg/(kg· day), the lumen diameter was significantly increased compared to that in control group [(86.0±5.0) μm vs. (69.0± 2.1) μm; P<0. 01, n = 6- 8]. The plasma 8-OHdG levels of ARB pretreatment groups were decreased. In immunohistochemical findings, 8-OHdG- and HIF-1α-containing cells in ARB pretreatment groups were decreased. Conclusion Brain ischemia and oxidative damage can be reversed by AT1 receptor blockade in normotensive rats after transient cerebral artery occlusion.     

The role of interleukin-8 produced by tumor induced fibroblasts in the development of cutaneous melanoma

Objective To determine the role of interleukin-8 (IL-8) produced by tumor induced fibroblasts in the development of cutaneous melanoma. Methods B16 melanoma cells induced L929 fibroblasts phenotype was transdifferentiated to myofibroblasts (MF) by co-culture in vitro. MF was monitored by morphology and immunophenotype for a-SMA. The level of IL-8 was detected by ELISA. The effect on B16 cell proliferation rate was estimated using MIT method in vitro. Melanoma implanting model was constructed in C57 mice. Results L929 MF phenotype could be modulated by B16 melanoma cells-derived transforming growth factor-β1 (TGF-β1) and elevated the levels of IL-8. L929 MF did not influence the B16 melanoma cells viability in vitro, but shortened the time of tumor formation and increased the incidence rates of tumors in C57 implanting model mice. Conclusion Fibroblasts can be activated by tumor cells and produce IL-8, which acts as an inflammatory cytokine promoting the development of cutaneous melanoma.     

The changes of neuronal activity of the zone incerta in a rodent model of Parkinson＇s disease

Objective To study the changes in neuronal activity of the zone incerta (ZI) following the unilateral lesion of the nigrostriatal pathway. Methods Eiectrophysiological recordings of ZI neurons were made in normal rats and in two groups of rats at different time intervals after injection of 6-hydrodopamine (6-OHDA) into the pars compacta of snbstantia nigra by extracellular recording in vivo. Results The results showed a significant increase in the mean firing rate of ZI during the second and fourth weeks after 6-OHDA lesion [-n=32, (3.6±2. 2)Hz, P<0.001; n=35, (9.3±6.6)Hz, P<0. 001, respectively] compared to that of normal rats [n=39, (9.2±5.2)Hz]. However, no significant change was observed between two groups of 6-OHDA-lesioned rats. With regard to firing pattern, 7.7%(3/39) of ZI neurons discharged regularly, 82.1% (32/39) irregularly and 10. 3% (4/39) in bursts in normal rats. During the second week after 6-OHDA lesion, the regular, irregular and bursting firing neurons recorded in the ZI were 9.4% (3/32), 59. 4% (19/32) and 31.3% (10/32), and during fourth week, the regular, irregular and bursting firing neurons were 14.3% (5/35), 57.1% (20/35) and 28. 6% (10/35), respectively. The firing pattern of the neurons in the three different groups did not change significantly. Conclusion These results suggest that the firing rate of ZI neurons in 6-OHDA-lesioned rats is increased significantly, which may contribute to the pathophysiology of Parkinson's disease.     

预应力锚索抗滑桩结构在处治滑坡中的应用

文章结合鹰阳关-莲塘二级公路K54+800处滑坡采用预应力锚索结构施工实践,介绍了预应力锚索抗滑桩结构在滑坡处治中的注意事项及使用效果.     

钢渣改良灰土挤密桩材料特性与应用研究

以河北省邢(台)衡(水)高速公路一期工程刚柔性长短桩复合地基加固湿地湖泊相软土试验段为依托,基于对钢渣材料特性的分析,提出在灰土中掺入钢渣形成钢渣改良灰土挤密桩对试验段内湖泊相软土进行加固。对钢渣改良灰土挤密桩材料改良特性进行了详细分析,通过配合比试验,对不同钢渣掺入量的材料强度变化规律进行了研究,以某一固定配比的钢渣灰土材料及相同灰土比的灰土材料为例,进行耐水性试验,对钢渣灰土材料的耐水性进行了分析,并通过对现场施工满28 d后的钢渣改良灰土挤密桩进行静力载荷试验及重型动力触探试验,对其承载力及成桩效果进行了评价,为钢渣改良灰土挤密桩的进一步应用提供技术参考。     

绿色之路 金龙同行——大金龙新能源客车服务“低碳盐城”

<正>又是秋冬季节,华东地区时常被雾霾笼罩,而盐城却成功逆袭,在刚刚公布的《江苏省2014年第三季度空气质量报告》中名列第一。近年来,盐城市践行"低碳"发展路线,大力发展公共交通,不断提高新能源客车所占的比例,环保效果已经凸现。作为盐城市最大的公共交通运输企业,盐城公交目前拥有运营线路61条,营运车辆870辆。据测算,运送相同数量的乘客,公交车与私家车相比能耗降     

考虑公务出行的拥挤收费效果研究

针对公务出行与非公务出行的差异性,分析了公务出行对拥挤收费的影响,提出了公务出行外部成本内部化措施,建立了内部化措施前后的拥挤收费模型。算例比较表明:在最优收费策略下,从路网总成本、社会福利、总出行量以及公共交通分担量方面,内部化后拥挤收费效果总是优于内部化前;二者的差距随着时间价值参数的增大而减小,随着公务出行所占比重的增大而增大,随着弹性灵敏参数的增大而增大。研究表明在时间价值越小,公务出行比重越大,以及弹性需求灵敏参数越大的条件下,内部化措施对于改善拥挤收费的效果就越明显。     

上海城市轨道交通近期线网建设的效果评价

结合上海轨道交通的实际情况,提出了城市轨道交通线网的建设效果评价体系和评价指标。评价体系和指标需综合考虑客运效果、线网布局、线路走向、与城市总体发展的适应性等诸多方面的因素。设计了涵盖5个层面,12个具体指标的评价体系。5个层面分别是城市交通服务、城市空间拓展、居民出行改善、线网自身效率和线网外部效益。同时,对票价票制、交通政策、土地开发、公交衔接和行车间隔等影响效果的不确定因素进行了分析,提出了确保线路网络效益的若干建议。     

湿陷性黄土地基冲击压实处治分析

为研究湿陷性黄土地基冲击压实处治效果,文章依据实际工程,在湿陷性黄土地基处治前后开展现场试验进行对比分析,通过分析沉降差和压实度检测结果,得出试验段冲击压实最佳碾压遍数为30遍。通过分析动力触探试验和土工试验结果可知,冲击压实后地基土密实度提高、压缩性下降,消除了湿陷性,整体稳定性得到明显提升。     

复合地基条件下沉管隧道块石垫层振密试验研究

依托深中通道深层水泥搅拌法(DCM)复合地基处理项目,对工程区域沉管隧道块石垫层开展陆上工艺振密试验研究,分析块石垫层振密时间、振动锤转速、激振力、垫层厚度、振沉量、变形模量等因素对DCM桩顶沉降、桩顶及桩间土应力变化特性的影响.试验结果表明:合理的液压振动锤转速、激振力、振密时间可以对块石层产生有效的振密;增加块石厚...