Study on chromatographic fingerprint of sarcandra glabra （Thunb.） by microwave-assisted extraction coupled to HPLC/DAD

Microwave-assisted extraction (MAE) was used for extraction of effective components of sarcandra glabra (Thunb.), and then chromatographic fingerprint of sarcandra glabra (Thunb.) was studied by high performance liquid chromatography/diode array detector (HPLC/DAD). The conditions of MAE were optimized by an orthogonal experiment, and then the authentication and validation of the chromatographic fingerprint were conducted. Nine peaks were identified as common peaks in the fingerprint chromatograms, and isofraxidin was considered as a reference compound and quantified. Relative standard deviations of retention time and peak area of each component were less than 3% and 8%, respectively. Similarity and difference analysis were conducted by use of PCA and relation coefficient. Twenty batches of sarcandra glabra (Thunb.) samples from two different producing areas could be classified into two different groups in the PCA model. The results showed that MAE-HPLC/DAD method was simple, efficient and stable for the study of complex chromatographic fingerprint of sarcandra glabra (Thunb.), which could provide more reliable and precise information for quality evaluation.     

Eukaryotic expression and biological activity analysis of neuroprotective peptide [Gly14]-Humanin

Objective To investigate the expression of neuroprotective peptide [Gly14]-Humanin (HNG) in eukaryotic cells by gene engineering technique and analyze its biological activity. Methods By means of asymmetrical primer/template, double stranded cDNA of HNG with FLAG in its C-terminal was obtained, which was cloned into the plasmid pcDNA3.1(-), and the resultant recombinant vector pcDNA3.1(-)/HNG-FLAG was transfected into PC12 cells. At the same time, the recombinant vector pcDNA3.1(-)/EGFP was transfected to control the efficiency of transfection. The expression of HNG in the cells was determined by immunocytochemistry. In order to analyze the biological activity of the expressed HNG, 25μM Aβ25-35 peptide was added to the culture medium of the transfected cells for 24h, then cell morphology, MTT assay and Hoechst 33258 staining were observed. Results The eukaryotic expression vector of pcDNA3.1(-)/HNG-FLAG was identified by enzyme digestion and sequencing. HNG was highly expressed in PC12 cells. After exposure of PC12 cells to 25μM Aβ25-35 for 24h, cell viability decreased to (65.8±5.3)%, and the dystrophic changes of neuritis and nuclei condensation were obvious. When cells were pre-transfected with pcDNA3.1(-)/HNG-FLAG, Aβ25-35-induced cell death and morphological changes of cells and nuclei were suppressed. In contrast, pre-transfected with empty vector did not protect cells from Aβ25-35-induced toxicity. Conclusion The eukaryotic expression vector for FLAG-tagged HNG was successfully constructed and expressed in PC12 cells. Expressed HNG has biological activity.     

鲨鱼鳃部共附生真菌中抗肿瘤活性化合物的研究

项目摘要：癌症是危害公众健康的宿敌．海洋生物共附生真菌参与宿主化学防御,产生的活性代谢产物新颖多样,是筛选具有抗肿瘤活性的新结构先导化合物的资源宝库．鲨鱼的鳃是一种“活的自航式微生物采样器”,栖居了包括共附生真菌在内的复杂微生物群落,是尚未被开发的海洋微生物新资源．而鲨鱼抗病力强、几乎不患癌症的现象一直引人注目,     

山东省水资源承载力评价及预测

结合山东省水资源的实际情况,采用主成分方法分析了水资源承载力的驱动因素,结果表明,山东省水资源承载力主要受经济、人口、水资源供需等因子的影响;并且根据2000年到2008年的统计数据,建立了多元回归模型对2015年山东省水资源需水量进行了预测。随着经济的发展,人口的增长,水资源供需不容乐观,应该加强水资源的管理,采取节水措施,使水资源可以持续利用。     

聚丙烯的共混改性研究

选用麦秸纤维对聚丙烯进行改性,研究了麦秸纤维含量和偶联剂种类对复合材料力学性能的影响。采用扫描电镜、体视显微镜分别观察了麦秸纤维的结构及其在聚丙烯中的分散性。结果表明,麦秸纤维经KH550表面处理剂处理后,含量为25％左右时,在聚丙烯基体中能保持均匀分散状态,且增强效果最佳。     

不要忽视这些小毛病

现象一：车辆加速“坐车”,油耗增加;冷起动不顺畅,甚至冷起动困难。 原因：这种故障原因比较多,长途行驶后出现这种问题很可能是所使用的油品有问题。现在加油站的油质量量参差不齐,如果汽油存放时间较长,或者汽油质量本身就有问题,会使汽油中胶质含量过多。车辆使用这种汽油后,进气门周围会产生大量胶质,使进气门运动不畅,严重的会使进气门在冷车时运动卡滞,发动机不容易起动。     

城市快速路交通事故特性分析及安全评价

以哈尔滨市3条快速路为研究对象,通过分析其道路交通事故统计数据,从时间分布、事故形态、事故成因等角度,探讨了城市快速路交通事故的特性。选取行车道宽度、交通饱和度、标志设施等9个指标,建立了城市快速路交通安全水平评价指标体系,进而提出了基于主成分分析法的快速路交通安全评价模型。应用案例表明,哈尔滨市和兴路的交通安全水平相对最好,公滨路交通安全水平较低,该结果与运用相对事故率法所得到的结论基本一致,从而验证了该模型的有效性。     

901-W无溶剂瓷釉涂料

901-W无溶剂瓷釉涂料是北京航材百慕新材料技术工程有限公司研发的产品。该产品是由环氧树脂、防锈颜料、改性固化剂、活性稀释剂、助剂等组成的环保、无毒无溶剂液体环氧涂料,适用于自来水厂、饮用水箱、船舶淡水舱、消防水箱、饮用水管线等钢结构防腐涂装。     

State inspection for transmission lines based on independent component analysis

Monitoring transmission towers is of great importance to prevent severe thefts on them and ensure the reliability and safety of the power grid operation. Independent component analysis (ICA) is a method for finding underlying factors or components from multivariate statistical data based on dimension reduction methods, and it is applicable to extract the non-stationary signals. FastICA based on negentropy is presented to effectively extract and separate the vibration signals caused by human activity in this paper. A new method combined empirical mode decomposition (EMD) technique with the adaptive threshold method is applied to extract the vibration pulses, and suppress the interference signals. The practical tests demonstrate that the method proposed in the paper is effective in separating and extracting the vibration signals.     

降低柴油机有害排放物的控制技术

随着汽车工业的发展,废气污染在严重地威胁着人类的生态环境,而我国的汽车工业还不太发达,废气污染十分严重,柴油机的有害排放物则是造成大气污染的一个主要来源.随着环境保护问题重要性的日趋增加,降低柴油机的有害排放物成为当今世界上柴油机发展的一个重要方向.