新型高科技系统检漏方法——美国TP公司荧光检漏技术简介

随着人类生活水平的提高和环保理念的不断提升，人们对日常交通运输工具—汽车的要求也在越来越高，除了安全、快捷、舒适、方便和尾气污染排放达标外，对汽车车辆各类循环系统的安全可靠运行和快速检修维护也提出了更高的要求。     

转向架构架内部焊缝超声波探伤方法的制定

为避免转向架构架内部焊缝引发的重大事故,研究制定转向架构架超声波探伤方法.利用加工有基准缺陷的实物模型进行试验,制定出判断缺陷的探伤法及实车转向架构架槽焊裂纹的判断流程.     

实时荧光定量PCR检测宫颈癌组织中HGF mRNA和c-Met mRNA的表达

目的检测宫颈癌组织中肝细胞生长因子(HGF)及其受体原癌基因c-Met的分子表达水平,并探讨其临床意义。方法采用实时荧光定量PCR(QRT-PCR)技术检测43例宫颈癌,30例宫颈上皮内瘤变Ⅲ和27例正常宫颈组织标本中HGF和c-Met的分子表达水平。结果 HGF mRNA和c-Met mRNA在宫颈癌组织中均呈高表达,其与宫颈癌患者临床分期、肿瘤直径大小、浸润深度、淋巴结转移有关(P均<0.05),而与肿瘤的分化程度无关(P>0.05)。HGF mRNA和c-Met mRNA之间存在正相关(r=0.666,P<0.01)。结论 QRT-PCR技术可对宫颈癌组织中的HGF mRNA和c-Met mRNA表达进行定量检测;HGF mRNA和c-Met mRNA的高表达与宫颈癌的侵袭转移有关。两者联合监测有利于早期诊断宫颈癌,为宫颈癌的治疗和预后提供参考。     

Microfluidics-based assay on the effects of microenvironmental geometry and aqueous flow on bacterial adhesion behaviors

Abstract A new microfluidic system with four different microchambers （a circle and three equilateral concave polygons） was designed and fabricated using poly（dimethylsiloxane） （PDMS） and the soft lithography method. Using this microfluidic device at six flow rates （5, 10, 20, 30, 40, and 50 μL/h）, the effects of microenvironmental geometry and aqueous flow on bacterial adhesion behaviors were investigated. Escherichia coli HB101 pGLO, which could produce a green fluorescent protein induced by L-arabinose, was utilized as the model bacteria. The results demonstrated that bacterial adhesion was significantly related to culture time, microenvironment geometry, and aqueous flow rates. Adhered bacterial density increased with the culture time. Initially, the adhesion occurred at the microchamber sides, and then the entire chamber was gradually covered with increased culture time. Adhesion densities in the side zones were larger than those in the center zones because of the lower shearing force in the side zone. Also, the adhesion densities in the complex chambers were larger than those in the simple chambers. At low flow rates, the orientation of adhered bacteria was random and disorderly. At high flow rates, bacterial orientation became close to the streamline and oriented toward the flow direction; All these results implied that bacterial adhesion tended to occur in complicated aqueous flow areas.The present study provided an on-chip flow system for physiological behavior of biological cells, as well as provided a strategic cue for the prevention of bacterial infection and biofilm formation.     

Rapid europium-sensitized fluorescent determination of ulifloxacin,the active metabolite of prulifloxacin,in human serum

A new fluorescent method was developed based on the ulifloxacin-europium（Ⅲ）-sodium dodecylbenzene sulfonate system for the determination of ulifloxacin,the active metabolite of prulifloxacin.Sodium dodecylbenzene sulfonate formed a ternary complex with ul     

铁道车辆轮对磁粉探伤方法的改进

通过分析铁道车辆轮对磁粉探伤机存在的不足，提出从设备配置、磁化原理及采用闭合移动式线圈法等方面的改进磁粉探伤方法的方案。通过样机测试试验，证明改进方案有效。     

钢轨数字探伤仪器的基本原理及其运用管理的探讨

钢轨探伤仪器探伤是钢轨检查的主要手段之一,目前已由模拟探伤发展到数字探伤,通过对数字探伤仪器与模拟探伤仪器的性能对比,说明其功能特点,并论述数字仪器的现场管理与常见问题处理,从而达到有效指导钢轨探伤的目的.     

氙弧灯和荧光紫外灯对面漆漆膜破坏性的比较

依据2项国家标准,分别采用氙弧灯和荧光紫外灯对不同颜色面漆漆膜的破坏性开展试验研究.试验结果显示,2种光源对面漆漆膜破坏性的区别主要表现在失光、色差和粉化方面.从成膜物质和试验条件方面分析试验结果的形成原因,比较2种光源对漆膜破坏的等效性.     

技术·产品

朔黄铁路钢轨探伤车完成首次正式上线运行 10月11—14日，朔黄铁路钢轨探伤车完成了对朔黄全线的钢轨探伤任务，标志着朔黄铁路公司钢轨探伤车的首次正式上线运行获得圆满成功。10月11日，钢轨探伤车执行肃宁北一黄骅港区间探伤任务。机辆分公司探伤车班组在出车前做了大量的准备工作，首先提前两天全面检查车辆和探伤设备，添加足够的耦合水和柴油，并根据线路情况对探轮膜的消耗作了充分预想及储备，此外还安排了经验丰富、操纵平稳的机车乘务员来执行牵引任务，完善的准备工作使首日的探伤任务得以顺利完成。     

HTK—90型荧光磁粉探伤机磁化参数的整定及相关的单片机中断和定时技术

简单说明ＨＴＫ－９０型荧光磁粉探伤机磁化电流幅度和作用时间的控制方法，介绍了幅度参数和时间参数的鉴定。