The role of interleukin-8 produced by tumor induced fibroblasts in the development of cutaneous melanoma

Objective To determine the role of interleukin-8 (IL-8) produced by tumor induced fibroblasts in the development of cutaneous melanoma. Methods B16 melanoma cells induced L929 fibroblasts phenotype was transdifferentiated to myofibroblasts (MF) by co-culture in vitro. MF was monitored by morphology and immunophenotype for a-SMA. The level of IL-8 was detected by ELISA. The effect on B16 cell proliferation rate was estimated using MIT method in vitro. Melanoma implanting model was constructed in C57 mice. Results L929 MF phenotype could be modulated by B16 melanoma cells-derived transforming growth factor-β1 (TGF-β1) and elevated the levels of IL-8. L929 MF did not influence the B16 melanoma cells viability in vitro, but shortened the time of tumor formation and increased the incidence rates of tumors in C57 implanting model mice. Conclusion Fibroblasts can be activated by tumor cells and produce IL-8, which acts as an inflammatory cytokine promoting the development of cutaneous melanoma.     

The changes of neuronal activity of the zone incerta in a rodent model of Parkinson＇s disease

Objective To study the changes in neuronal activity of the zone incerta (ZI) following the unilateral lesion of the nigrostriatal pathway. Methods Eiectrophysiological recordings of ZI neurons were made in normal rats and in two groups of rats at different time intervals after injection of 6-hydrodopamine (6-OHDA) into the pars compacta of snbstantia nigra by extracellular recording in vivo. Results The results showed a significant increase in the mean firing rate of ZI during the second and fourth weeks after 6-OHDA lesion [-n=32, (3.6±2. 2)Hz, P<0.001; n=35, (9.3±6.6)Hz, P<0. 001, respectively] compared to that of normal rats [n=39, (9.2±5.2)Hz]. However, no significant change was observed between two groups of 6-OHDA-lesioned rats. With regard to firing pattern, 7.7%(3/39) of ZI neurons discharged regularly, 82.1% (32/39) irregularly and 10. 3% (4/39) in bursts in normal rats. During the second week after 6-OHDA lesion, the regular, irregular and bursting firing neurons recorded in the ZI were 9.4% (3/32), 59. 4% (19/32) and 31.3% (10/32), and during fourth week, the regular, irregular and bursting firing neurons were 14.3% (5/35), 57.1% (20/35) and 28. 6% (10/35), respectively. The firing pattern of the neurons in the three different groups did not change significantly. Conclusion These results suggest that the firing rate of ZI neurons in 6-OHDA-lesioned rats is increased significantly, which may contribute to the pathophysiology of Parkinson's disease.     

探讨沥青路面局部渗水的原因及处理方法

通过武汉市汉蔡高速公路沥青路面出现的局部渗水现象,分析沥青路面产生局部渗水的原因及处理的必要性,提出对沥青路面局部渗水的处理原则及处理方法。     

拉力型锚索锚固段长度的一种确定方法

文章分析了锚杆拉拔试验的结果,提出了非全长粘结型锚索锚固段剪应力沿长度分布的二次抛物线模式,以及确定锚固段长度的合理方法,并以工程实例说明了文中提出的锚索锚固段长度计算方法的合理性.     

准动态分析法在工程船舶锚泊定位系统设计中的应用研究

工程船舶在海上作业时都需配备定位系统,如何配置安全可靠、满足作业要求和具有市场竞争力的锚泊定位系统已成为探讨热点。通过对现行锚泊定位系统分析的几种基本方法的理论特点及应用特性比较,确定应用准动态方法对工程船进行锚泊定位分析的可行性,并阐述了准动态分析方法的基本理论及特点,与全动态分析方法进行了理论对比分析,从而进一步体现了准动态方法应用于工程船舶锚泊定位的合理性。以某工程船为例,分别应用准动态分析软件ARAINE及全动态分析软件DEEPC进行锚泊定位计算对比分析,论证了应用准动态方法分析工程作业船舶锚泊定位的优势及特点。从理论分析及实际应用角度充分证明了,应用准动态方法对工程船进行锚泊定位分析既保证工程精度要求,又可显著提高计算效率,具有明显优势。     

健美操选项课学生创新能力培养的研究与实践

采用文献资料法、问卷调查法、测量法、比较研究法、数理统计等方法,对辽宁省交通高等专科学校2014级健美操选项课学生进行调查,旨在掌握其创新能力现状、存在的问题、产生的原因,为采取干预对策,优化创新能力培养途径、方法,提高其创新能力提供科学依据。     

农村公路布局影响因素及其规划方法的探讨

当前,我国社会经济发展迅速,区域经济的发展离不开交通系统的升级,因此,有效进行农村公路建设,有利于改变农村经济发展落后的局面。针对印江县农村公路网现状,具体分析了农村公路布局影响因素及其规划方法。     

阳紫大桥设计与计算分析

桥梁作为陆地交通中的重要组成部分,很多技术人员在其出现之初就投入大量的精力研究其结构、材料和施工方法等。自锚式悬索桥作为一种新型桥梁,以其独有的特点迅速在世界各地得到广泛建造。以阳紫大桥为例,介绍了自锚式悬索桥的桥墩、主梁和吊索等部分的设计要点和计算方法,对以后类似工程设计和建造工作有一定的借鉴价值。     

大粒径碎石路基压实质量控制方法研究

以大粒径碎石路基的工程特性和施工技术为研究目标,在室内试验研究大粒径碎石填料的颗粒组成、工程特性的基础上,通过现场试验,对大粒径碎石路基质量控制关键技术展开研究,对不同质量控制方法提出了保证检测精度的措施,以适应大粒径碎石路基检测的需要。     

某板梁桥支座更换的施工监控方法

桥梁支座更换施工过程中其梁体受到下部的顶升力和上部的车辆荷载以及梁体本身自重等多因素的影响,梁体顶升过程中梁体的位移、应变(应力)的变化必须可知、可控。通过对某桥板梁位移、应变的监控来了解位移、应变的变化情况,指导工程施工。