Induction of apoptosis and inhibition of proliferation of C6 glioma cells in vitro by tamoxifen

Objective To investigate the anti-tumor effect and mechanism of tamoxifen on rat C6 glioma cells. Methods C6 cells were cultured in Dulbecco＇s modified Eagle＇s medium （DMEM） with 3% fetal calf serum （FCS）, and treated with tamoxifen of different concentrations, i.e. group A （1.25 μmol/L）, group B （2.50 μmol/L）, group C （5. 00 μmol/L）, group D （10. 00 μmol/L）, group E （20. 00 μmol/L） and control group （0. 00 μmol/L）. Morphological changes, MTT assay and 5-bromo-2＇-deoxyuriding labeling ratio were assessed. Apoptosis was observed by flow cytometry. Results C6 cells treated with different doses of tamoxifen for 24, 48, and 72 hours became irregular in shape, while cells treated with vehicle grew normally. MTT assay showed that tamoxifen did not suppress C6 cell growth until 72 hours after treatment. Seventy-two hours after treatment, there were significant differences in cell viable rate between group A versus groups C, D and E; so did group B versus group D as well as group E （P〈 0.05 ）. BrdU incorporation assay indicated significant difference of BrdU labbled index （BrdU LI） among groups A, C, E and control group 48 hoers after treatment （P〈0.05）. And the BrdU LI decreased with the increased concentration of tamoxifen. Flow cytometry （FCM） showed significant difference between treated group and control group at 24, 48, and 72 hours after treatment （P〈0.05）. Conclusion Tamoxifen significantly suppresses the growth of C6 glioma cells in a time- and dose-dependent manner. The mechanism of tamoxifen suppressing C6 glioma cells may be inhibiting proliferation and inducing apoptosis. Therefore, tamoxifen can be a candidate as a chemotherapy agent for glioma.     

THEORY AND APPLICATION OF M-BAND WAVELET TRANSFORM

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在VC6中显示ACCESS数据库中图像字段的新方法

介绍在VC6中用图像控件显示ACCKSS数据库中OLE字段的位图。首先阐述CLongBinary类与CByteArray类的区别；接着介绍图像控件的使用；最后叙述如何用VC6调用ACCESS数据库中的OLE字段，使其在Imase控件上显示位图，以及如何将位图存人数据库中。     

南京地铁宁溧线信号控制4/6节编组列车混合运行技术方案

根据南京地铁宁溧线和机场线贯通运营的要求,空港新城江宁站至无想山站小交路需要支持4/6节编组列车混合运行.为此,信号系统需对车载信号和轨旁信号进行特殊设计,除在轨旁增加混合运行控制设备,并配置不同的车载数据库,实现自动识别列车编组外,还需对不同编组列车的定位、超速防护、对标停车、试车线测试以及站台门联动进行设计,增加混合运行功能,实现4/6节编组列车的安全运行控制.     

钛合金Ti-6Al-4V ELI厚板多层多道焊试验与数值模拟

对2块厚度为24mm的Ti-6Al-4VELI厚板进行夹角为171.98°的多层TIG焊接试验,基于SYSWELD软件平台,在焊板两端完全刚性固定的装夹条件下数值模拟计算焊接温度场、应力场和焊接引起的变形.计算结果表明,焊接温度场的变化规律符合实际,焊缝区的拉伸残余应力最大,热影响区的压缩残余应力较大.焊接数值模拟得到的残余应力场与试验测量值能较好地吻合,验证了数值模拟模型的准确性.该数值模拟方法可应用到大深度Ti-6Al-4VELI载人舱强度计算中的焊缝残余应力模拟中.     

Real-world gaseous and particle emissions of a Bi-fuel gasoline/CNG Euro 6 passenger car

The objective of the present study is the assessment of the environmental impact of a bivalent (bi-fuel) vehicle, running either on gasoline or compressed natural gas (CNG). To that aim, a Euro 6 passenger car was tested under various real-world driving conditions. In order to cover the full range of conventional powertrains currently in the market, the tests were also repeated on a Euro 6 diesel passenger car. Both cars were driven in two routes, the first complying with the regulation limits and the second going beyond them. Carbon monoxide (CO), nitrogen oxides (NO_x) and particle number (PN) emissions were recorded using a Portable Emissions Measurement System (PEMS). Apart from the aggregated emission levels, in g/km, the exact emission location along the route was also assessed. Natural gas proved beneficial for CO and PN emissions, the level of which always remained below the respective legislation limits. On the other hand, under the dynamic driving conditions with gasoline, the relevant limits were exceeded. Cold start, occurring at the beginning of the urban part, and motorway driving were identified as major contributors to total emissions, especially in gasoline mode. However, the application of natural gas was associated with a penalty in NO_x emissions, which were significantly increased as compared to gasoline. Local peaks within the urban part were identified in CNG mode. In any case, the diesel vehicle was by far the highest NO_x emitter.     

机车检测系统中JT-6管理的设计与实现

对机车检测系统中的JT-6管理子系统作了介绍,重点分析了JT-6报活,JT-6施修的功能及设计思想,简要介绍了故障字典、JT-6查询、JT-6验收等功能,并阐述了其关键技术。     

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了解信号交叉口下游车辆的车头时距分布特征是进行城市道路控制、通行能力计算、交通安全分析等的基础.利用NC200、MC5600对信号交叉口下游不同断面不同车道的车头时距进行调查.采用χ2检验法对信号交叉口下游车辆的车头时距分布进行拟合,得到高峰时段和平峰时段的不同断面、不同车道车头时距分布.研究结果表明:随着高峰时段向平峰时段过渡,以及车辆由交叉口向下游移动,车道的车头时距在移位负指数分布和M3分布之间进行过渡.     

区域间拓扑匹配的Chord6模型研究

为了解决P2P网络中存在的不同地址前缀区域拓扑不匹配,即物理网络中相邻的两个区域在覆盖网络中相距很远,反之亦然,以及由此造成的底层路由效率低下的问题.本文采用IPv6地址层次性和其泛播通信机制对节点标识符的重新设计,改进了Chord6模型.该方法不仅使得各个区域间具有拓扑一致性,而且使得路由表具有地址聚合的特性.实验表明,该方法与Chord6相比,在覆盖网络中路由跳数上减少了约10％,在物理网络中端到端的延时降低了约10％.     

110kV GIS变频串联谐振耐压试验分析

分析了广珠线金塘110kV GIS交流耐压试验中净化和耐压2个阶段,并给出了该项试验的判定方法,提出了耐压试验注意事项和建议。