单纯疱疹病毒1型糖蛋白D核酸疫苗的构建及初步免疫观察

目的　构建用于防治单纯疱疹的核酸疫苗并观察其免疫效果。方法　用PCR反应 ,从HSV 1F株基因组DNA中扩增出 gD蛋白的全部编码序列 ,将其插入 pcDNA 3 1 (+)的PCMV下游 ,构建HSVgD核酸疫苗 ,并用酶切分析和测序鉴定 ;通过中和试验、ELISA方法和攻击试验检测用 pLy D三次肌肉接种小鼠的免疫效果。结果　经鉴定证实了HSV gD核酸疫苗 pLy D的构建 ;实验小鼠产生了对HSV 1特异性的抗体 (抗体滴度 :ELISA法 1∶2 0 3,中和试验法 1∶37) ,并经受了HSV 1病毒的攻击 (存活率 :70 % )。结论　成功构建出了HSV gD核酸疫苗pLy D ,用其接种小鼠可以诱导产生特异性免疫反应 ,并具有良好的保护作用。     

土工离心模型试验原理及误差问题

土工离心模型试验已广泛用于土木工程的各个领域,本文介绍其基本原理,讨论其误差问题。     

相关背景知识在微积分教学中的有效运用

结合教学实践,讨论了实数的引入,Newton-Leibniz公式,无穷级数,函数的可积性准则这几个相关的微积分背景知识在教学中的有效运用。     

电力机车在车检测的现状和改进建议

对电力机车不解体检测的部件、测试原理、测试方法和测试设备进行了综述,分析目前在车检测中存在的问题,并提出了相应的改进建议。     

Origin–destination missing data estimation for freight transportation planning: a gravity model-based regression approach

This paper develops a log-linear regression approach to estimate missing data in a sparse origin–destination (O–D) matrix assuming the sampled or observed O–D trips follow a good gravity pattern. The approach is tested with randomly selected samples from the known portions of 1997, 2002, and 2007 US Commodity Flow Survey (CFS) O–D value and tonnage matrices and validated with 2007 US O–D tonnage matrix at the state level. The missing data are also estimated for the 2007 CFS tonnage matrix with the best intercept and coefficients obtained using all known entries of the matrix. The concept of the approach can be extended beyond the gravity model to any strong mathematical pattern embedded in the known set of a sparse O–D matrix to estimate its missing cells.     

Strategic de-confliction in the presence of a large number of 4D trajectories using a causal modeling approach

This paper presents a strategic de-confliction algorithm based on causal modeling developed under the STREAM project and launched under the umbrella of the Single European Sky ATM Research (SESAR) Program. The basic underlying concept makes use of the enriched information included in the Shared Business Trajectories (SBTs) of the flights prior to takeoff (or in the Reference Business Trajectories (RBTs) if the flight is airborne) to allocate conflict-free trajectories in a traffic planning phase that should lead to an actual conflict-free scenario in the flight execution phase in the absence of flight and/or network uncertainties. The proposed approach could decrease the workload of the air traffic controllers, thus improving the Air Traffic Management (ATM) capacity while meeting the maximum possible expectations of the Airspace Users’ requirements in terms of horizontal flight efficiency. The main modules of the implemented system are also presented in this paper; these modules are designed to enable the processing of thousands of trajectories within a few seconds or minutes and encompass a global network scope with a planning horizon of approximately 2–3 h. The causal model applied for network conflict resolution and flight routing allocation is analyzed to demonstrate how the emergent dynamics (i.e., domino effects) of local trajectory amendments can be efficiently explored to identify conflict-free Pareto-efficient network scenarios. Various performance indicators can be taken into account in the multi-criteria optimization process, thus offering to the network manager a flexible tool for fostering a collaborative planning process.     

盾构隧道开挖对既有建筑物沉降分析

地铁建设是解决城市交通拥堵问题的有效途径。但是,如果施工措施不当,可能会导致地面沉陷、基坑垮塌、隧道破坏、周边建筑物损害、地下管线损害等事故。该文针对这一问题,参考相关文献,将结构、土体和地铁盾构施工综合进行分析,探讨了多种施工因素对邻近建筑物或构筑物的影响,以确保在隧道盾构正常施工的同时,尽量减小施工对邻近建筑物或构筑物的不利影响。为了分析城市地铁隧道盾构施工对邻近建筑的影响,立足郑州市实际情况,采用数值仿真分析软件FLAC3D,对盾构施工对邻近建筑的影响进行了数值模拟,讨论了隧道与建筑物间距对地表最大沉降及基础差异沉降的影响。     

快速路出入口布置原则及适应性浅议

城市快速路出入口是城市快速路系统的重要组成部分。该文分析了快速路设计规划中关于出入口布置类型、设置原则及适应性分析等内容,初步概括了快速路出入口的主要设计特点、要点,可供今后快速路实施作参考。     

浅谈桥梁加固

该文回顾了不同加固方式的基本概念,介绍了不同加固方式的技术特点、适用范围,对不同加固方式进行了评述。     

减低交通荷载引起地面振动的三维数值分析

针对高速铁路和高等级公路的运行产生振动波可能带来严重的噪音和地面振动等环境问题,运用LS-DYNA软件,选用更适合岩土介质的弹塑性模型,对高速列车作用下地面振动及连续墙挡板的防护能力进行三维数值模拟分析。研究了5～20 Hz荷载下混凝土桩组成的不同尺寸挡板的减振效果,并与日本某现场试验结果比较。结果表明,尽管无填充壕沟的防护效果最佳,但钢筋混凝土桩墙更具可操作性,稳定性,且混凝土桩减振挡板可以显著减低地面的振动加速度幅值,与波长有关。挡板长度和深度直接影响混凝土减振挡板的防护效果和防护范围,挡板深度对防护效果的影响更为明显,挡板宽度的尺寸主要影响防护范围。