Apoptin原核表达载体的构建及在大肠杆菌中的表达

目的构建Apoptin的原核表达载体,并制备抗原物质Apoptin融合蛋白。方法在获得Apoptin融合基因的基础上,成功构建了Apoptin的高效原核表达载体pET-28a( )-Apoptin,将该质粒转化至大肠杆菌E.coliBL21(DE3)受体菌中,以IPTG对其进行诱导表达,聚丙烯酰胺凝胶电泳分析目的蛋白。结果转化有Apoptin的原核表达载体pET-28a( )-Apoptin的大肠杆菌E.coliBL21(DE3)经IPTG诱导后,经SDS-PAGE分析,在相对分子质量约17 000的位置出现目的蛋白条带,大小与Apoptin融合蛋白一致。结论Apoptin原核表达载体pET-28a( )-Apoptin能够表达出Apoptin融合蛋白,为进一步的Apoptin研究和制备Apoptin抗体奠定了基础。     

YB28-630A双动薄板冲压机电气控制系统设计

介绍了双动薄板冲压机控制系统的改造方案,可编程控制器的硬、软件系统的组成,软件的设计思想.以及系统中压力信号的检测和处理方法。     

Observations of the Fawn Trough Current over the Kerguelen Plateau from instrumented elephant seals

Due to its great meridional extent and relatively shallow depths, the Kerguelen Plateau constitutes a major barrier to the eastward flowing Antarctic Circumpolar Current in the Indian sector of the Southern Ocean. While most of the Antarctic Circumpolar Current transport is deflected north of the Kerguelen Islands, the remainder ( 50 Sv, 1 Sv = 10⁶ m³ s^− 1) must pass south of the islands, most probably through the Fawn and Princess Elizabeth Troughs. However, the paucity of finely resolved quasi-synoptic hydrographic data in this remote and infrequently sampled area has limited the progress in our knowledge of the regional circulation. Since 2004, a new approach using elephant seals from the Kerguelen Islands as autonomous oceanographic profilers has provided new information on the hydrography over the Kerguelen Plateau, covering the entire Antarctic Zone between the Polar Front and Antarctica, with a mean along-track resolution of about 25 km. These finely resolved bio-logged data revealed details of a strong northeastward current found across the Fawn Trough (sill depth: 2600 m; 56°S, 78°E). This so-called Fawn Trough Current transports cold Antarctic waters found mostly south of the Elan Bank, between the Ice Limit (58°S) and the Antarctic Divergence (64°S) in the eastern Enderby Basin, toward the Australian–Antarctic Basin. Our analysis also demonstrates that the Deep Western Boundary Current, which carries cold Antarctic water along the eastern flank of the southern Kerguelen Plateau collides with Fawn Trough Current at the outlet of the Fawn Trough sill. In other words, the Fawn Trough constitutes a veritable bottleneck, channelling the quasi-totality of the Antarctic Circumpolar flow found south of the Polar Front. Thanks to the unprecedented fine resolution of seal-borne data, a branch of flow centered at the Winter Water isotherm of 1 °C is also revealed along the northern escarpment of the Elan Bank, and then along the southern edge of Heard Island. Further analysis of different supplementary data reveals a complex circulation pattern in the entire Enderby Basin, with several distinctive branches of flow being strongly controlled by prominent topographic features such as the Southwest Indian Ridge, Conrad Rise, Elan Bank, and Kerguelen Plateau. This newly emerged frontal structure refines considerably previous large-scale circulation schematics of the area.     

智能马达控制中心在海洋平台设计中的应用

介绍一种利用新型智能电机过载继电器E3 PLUS、现场通信总线DeviceNet及PLC等技术相结合的船舶智能马达控制系统,阐述智能马达控制中心(Smart MCC)的工作原理、功能及应用实例,该系统可以提供电机多种故障检测和诊断功能以及计算机集中控制,使马达控制系统逐步向自动化集成型发展,系统的性能和可靠性高,能最大限度地降低船舶整体运行成本,提高运行效率.     

自卸式远洋散装水泥运输船特殊结构强度分析研究

以远洋散装水泥运输船为研究对象,采用三维有限元分析方法及ＤＮＶ船级社的ＳＥＳＡＭ结构计算软件,从船体结构强度、船舶振动预报和船体结构温度应力等方面对该型船结构设计的多项技术难点进行了详细的计算和评估。并且着重对大型散装水泥运输船间断内底板、中纵舱壁以及高腹板旁桁材等特殊结构的受力状态及应力分布进行分析和探讨。为自卸式散装水泥运输船的结构优化设计提供技术支撑。     

4MlE在施工项目管理中的拓展

通过对施工项目管理中"4M1E"的分析及其在当今项目管理中的拓展,为企业进行其项目管理提供理论依据.     

Pro/E在减速顶中的应用

通过对三维建模软件Pro／E各种强大功能的简要介绍，指出Pro／E在当今生产行业中的重要作用，同时详细介绍了Pro／E在减速顶行业中的广泛应用。     

200km/h EMU轴箱体加工关键尺寸控制

介绍了200 km/h EMU轴箱体的加工工艺,对所用的设备及相关的工艺控制要求分别进行了阐述,对关键尺寸制定了详细的加工工艺,保证了产品质量。     

钢铁公司用200t专用平车的研制

介绍了200t钢板运输专用平车的研制过程、主要结构、技术参数及运用情况。     

汽车电子电气架构设计与优化

阐述汽车电子电气架构技术与发展趋势,设计与优化的指导思想、需求信息、所用工具和工作流程,以及两个标准的系统级电子电气架构。介绍汽车电子电气架构设计与优化的优点,对我国汽车行业研发该项技术提出建议。