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11.
By taking advantage of the user-defined load subroutine (loadud) and the user common subroutine (usercomm) in LS-DYNA, the authors proposed a new coupled approach for simultaneously calculating structural damage and the planar 3DOF ship motions in ship collisions. The coupled procedure aimed at predicting the detailed structural damage together with reasonable global ship motions. This paper extends the method to consider the full 6DOF ship motions; thus, ship collision as well as grounding accidents can be properly handled. This method is particularly useful for design purposes because the detailed ship hull profile is not needed.A traditional ship maneuvering model is used for the in-plane surge, sway and yaw degrees of freedom with a series of nondimensional coefficients determined from experiments. It is assumed that the out-of-plane degrees of freedom are not coupled with the in-plane ship motions, and there is no coupling among roll, pitch and heave motions. The implementation is verified through free decay tests, and the obtained natural periods show good agreement with theoretical results.Several collision and grounding cases are simulated in which a supply vessel crashes into rigid plates with different orientations. The effects of the roll motion, the heave and pitch motions and the full 6DOF motions are studied. The results are compared with those from a 6DOF decoupled method. Ship motions through the proposed method compare reasonably well with SIMO results. It is found that several consecutive impacts may occur in the simulation of one collision case due to the periodic motions. This is not taken into account in the decoupled method, which makes this method unconservative. 相似文献
12.
目的构建Apoptin的原核表达载体,并制备抗原物质Apoptin融合蛋白。方法在获得Apoptin融合基因的基础上,成功构建了Apoptin的高效原核表达载体pET-28a( )-Apoptin,将该质粒转化至大肠杆菌E.coliBL21(DE3)受体菌中,以IPTG对其进行诱导表达,聚丙烯酰胺凝胶电泳分析目的蛋白。结果转化有Apoptin的原核表达载体pET-28a( )-Apoptin的大肠杆菌E.coliBL21(DE3)经IPTG诱导后,经SDS-PAGE分析,在相对分子质量约17 000的位置出现目的蛋白条带,大小与Apoptin融合蛋白一致。结论Apoptin原核表达载体pET-28a( )-Apoptin能够表达出Apoptin融合蛋白,为进一步的Apoptin研究和制备Apoptin抗体奠定了基础。 相似文献
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Objective To study the changes in neuronal activity of the zone incerta (ZI) following the unilateral lesion of the nigrostriatal pathway. Methods Eiectrophysiological recordings of ZI neurons were made in normal rats and in two groups of rats at different time intervals after injection of 6-hydrodopamine (6-OHDA) into the pars compacta of snbstantia nigra by extracellular recording in vivo. Results The results showed a significant increase in the mean firing rate of ZI during the second and fourth weeks after 6-OHDA lesion [-n=32, (3.6±2. 2)Hz, P<0.001; n=35, (9.3±6.6)Hz, P<0. 001, respectively] compared to that of normal rats [n=39, (9.2±5.2)Hz]. However, no significant change was observed between two groups of 6-OHDA-lesioned rats. With regard to firing pattern, 7.7%(3/39) of ZI neurons discharged regularly, 82.1% (32/39) irregularly and 10. 3% (4/39) in bursts in normal rats. During the second week after 6-OHDA lesion, the regular, irregular and bursting firing neurons recorded in the ZI were 9.4% (3/32), 59. 4% (19/32) and 31.3% (10/32), and during fourth week, the regular, irregular and bursting firing neurons were 14.3% (5/35), 57.1% (20/35) and 28. 6% (10/35), respectively. The firing pattern of the neurons in the three different groups did not change significantly. Conclusion These results suggest that the firing rate of ZI neurons in 6-OHDA-lesioned rats is increased significantly, which may contribute to the pathophysiology of Parkinson's disease. 相似文献
14.
目的研究弥漫性轴索损伤(DAI)后患者外周血CD4+/CD8+T细胞比值及炎症因子IL-6、IL-10水平的变化,探讨影响DAI患者预后的相关危险因素及可能的预测因子。方法收集115例DAI患者的相关临床资料及伤后至少6个月的随访结果,并将采集的患者外周血采用流式细胞仪检测CD4+、CD8+T细胞及其比值,ELISA法检测IL-6、IL-10水平,对免疫炎症指标及临床资料进行单因素和多因素分析,多因素分析采用Logistic回归分析。结果DAI后合并其他脑损伤、瞳孔改变、伤后昏迷时间及入院时GCS评分是影响患者预后的危险因素,而其他因素则与预后不相关。CD4+/CD8+T淋巴细胞比值降低是DAI后发生ARDS/ALI的危险因素,CD4+/CD8+T淋巴细胞比值与患者预后关系不大,而ARDS/ALI的发生则是影响DAI患者预后的重要危险因素。IL-6增加与DAI后ARDS/ALI密切相关,可作为ARDS/ALI发生的预测因子,其水平是患者预后的危险因素,而IL-10则在此过程中与ARDS/ALI不相关,且与患者预后无关。结论 CD4+/CD8+T淋巴细胞比值降低是DAI后发生ARDS/ALI的危险因素,IL-6可作为ARDS/ALI发生的预测因子。DAI后与患者预后相关的危险因素包括合并其他脑损伤、瞳孔改变、伤后昏迷时间、入院时GCS评分、IL-6水平及并发ARDS/ALI。 相似文献
15.
徐文燕 《广东交通职业技术学院学报》2006,5(1):51-53
下一代网络(NGN)是目前网络发展的趋势,是今后通信业务和互联网业务的核心,其核心承载将使用IPv6技术。作为IPv6内在组成部分的移动IPv6技术,对于下一代网络,尤其是对于下一代移动通信网络,有着极其重要的影响。通过移动IPv6技术,可以各种接入方式,将宽带、移动互联网和现有的无线系统等都无缝地集成到IP层,通过一种网络基础设施提供所有通信和信息服务,即是下一代网络发展的方向,也是移动IPv6的核心优势。 相似文献
16.
切削加工中切屑成形是一种典型的大变形问题,它涉及到材料非线性、几何非线性以及边界非线性等问题。本文利用DEFORM-2D对钛合金(Ti6Al4V)进行了锯齿状切屑形成过程的有限元模拟,分析了切削过程中的应力场、应变场及温度场,探讨了锯齿状切屑的形成机理,获得了切削参数对锯齿状切屑形态的影响规律,为优化切削参数、提高刀具寿命提供了参考。 相似文献
17.
mTOR/P70S6K信号通路在宫颈癌组织中的表达及其临床意义 总被引:2,自引:0,他引:2
目的 研究mTOR/P70S6K信号通路中哺乳动物雷帕霉素靶蛋白(mTOR)及其底物激酶(P70S6K)在宫颈癌中的表达及其在宫颈癌发生发展中的作用.方法 用RT-PCR和免疫组织化学法检测正常宫颈、宫颈癌中mTOR和P70S6K激酶的表达.结果 与正常组织相比,宫颈癌中的mTOR和P70S6K激酶在mRNA及蛋白水平表达均显著增加(P<0.05),且两者的表达具有显著的正相关性(r=0.746,P<0.001).结论 mTOR/P70S6K信号转导通路在介导宫颈癌的发生、发展的过程中起到重要作用. 相似文献
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Observations of the Fawn Trough Current over the Kerguelen Plateau from instrumented elephant seals 总被引:2,自引:1,他引:1
Fabien Roquet Young-Hyang Park Christophe Guinet Frdric Bailleul Jean-Benoît Charrassin 《Journal of Marine Systems》2009,78(3):377
Due to its great meridional extent and relatively shallow depths, the Kerguelen Plateau constitutes a major barrier to the eastward flowing Antarctic Circumpolar Current in the Indian sector of the Southern Ocean. While most of the Antarctic Circumpolar Current transport is deflected north of the Kerguelen Islands, the remainder ( 50 Sv, 1 Sv = 106 m3 s− 1) must pass south of the islands, most probably through the Fawn and Princess Elizabeth Troughs. However, the paucity of finely resolved quasi-synoptic hydrographic data in this remote and infrequently sampled area has limited the progress in our knowledge of the regional circulation. Since 2004, a new approach using elephant seals from the Kerguelen Islands as autonomous oceanographic profilers has provided new information on the hydrography over the Kerguelen Plateau, covering the entire Antarctic Zone between the Polar Front and Antarctica, with a mean along-track resolution of about 25 km. These finely resolved bio-logged data revealed details of a strong northeastward current found across the Fawn Trough (sill depth: 2600 m; 56°S, 78°E). This so-called Fawn Trough Current transports cold Antarctic waters found mostly south of the Elan Bank, between the Ice Limit (58°S) and the Antarctic Divergence (64°S) in the eastern Enderby Basin, toward the Australian–Antarctic Basin. Our analysis also demonstrates that the Deep Western Boundary Current, which carries cold Antarctic water along the eastern flank of the southern Kerguelen Plateau collides with Fawn Trough Current at the outlet of the Fawn Trough sill. In other words, the Fawn Trough constitutes a veritable bottleneck, channelling the quasi-totality of the Antarctic Circumpolar flow found south of the Polar Front. Thanks to the unprecedented fine resolution of seal-borne data, a branch of flow centered at the Winter Water isotherm of 1 °C is also revealed along the northern escarpment of the Elan Bank, and then along the southern edge of Heard Island. Further analysis of different supplementary data reveals a complex circulation pattern in the entire Enderby Basin, with several distinctive branches of flow being strongly controlled by prominent topographic features such as the Southwest Indian Ridge, Conrad Rise, Elan Bank, and Kerguelen Plateau. This newly emerged frontal structure refines considerably previous large-scale circulation schematics of the area. 相似文献