V(D)J重组起始阶段的研究进展与展望

重组激活基因(recombination activating gene)1和2编码的RAG1和RAG2蛋白通过对V(D)J重组起始阶段的调节作用,使得抗原受体基因重排严格地按组织、细胞发育阶段进行。本文将就RAG蛋白结合和催化断裂抗原受体基因机制作简要介绍。这些新发现明确了体内V(D)J重组发生的部位以及其出现错误时可能发生的地方,提示V(D)J重组机制不仅对淋巴细胞正常发育起关键性作用,而且对基因组不稳定性和淋巴系统恶性肿瘤的发生也具有重要意义。     

肾母细胞瘤中ERCC1、TUBB3、TOP2A mRNA的表达及临床意义

目的探讨核苷酸切除修复交叉互补基因(excision repair cross complementation 1,ERCC1)、3型β微管蛋白编码基因(ClassⅢβ-tubulin,TUBB3)和DNA拓扑异构酶Ⅱα(TopoisomeraseⅡA,TOP2A)mRNA在小儿肾母细胞瘤组织中的表达及临床意义。方法收集18例肾母细胞瘤组织标本及14例癌旁组织标本,通过分支-DNA液相芯片法检测标本中ERCC1、TUBB3、TOP2A mRNA的表达水平,并分析上述基因表达与肾母细胞瘤临床病理的关系。结果肾母细胞瘤组织中ERCC1、TUBB3和TOP2A高表达率分别为44.4%、50.0%和66.7%,均显著高于癌旁组织的7.1%、7.1%和14.3%,差异有统计学意义(P<0.05)。ERCC1高表达率与肾母细胞瘤临床分期和病理类型有关(P<0.05),TOP2A高表达率与肾母细胞瘤临床分期相关(P<0.05)。结论小儿肾母细胞瘤组织中ERCC1、TUBB3和TOP2A mRNA表达高于癌旁组织,可能参与肾母细胞瘤的发生发展过程。     

Implications of globalization for co 2 emissions from transport

Transport accounts for about 25% of global CO 2 emissions. Transport activities are on the rise in the coming decades. Would associated CO 2 emissions move upwards as well, and at what rate? The present paper explores the future of these CO 2 emissions, starting from four scenarios for global transport. Considering fuel consumption, energy efficiencies in transport, occupancy rates of transport means, size of cars on the market, and possible environmental policies we find CO 2 emissions are persistently increasing, especially in the less wealthy areas of the world. In Europe, policies that attempt to control mobility and also limit CO 2 emissions may succeed in reducing emissions growth by about 30%. Efforts to increase energy efficiency of transport, in particular road transport, would contribute most to such reduction.     

新型皮带修补技术——蒂普拓普T2胶枪修补系统的应用

使用热硫化热补方式,修理皮带撕裂工作量大,维修时间长,严重影响企业生产.新的皮带机修补技术T2胶枪应运而生.阐述了该T2胶枪的技术原理、T2胶枪的结构、特点和使用工序.通过应用实例,证明该技术取得良好的经济效益,为用户提供了一种创新的维修理念.     

Variations in ectoenzymatic hydrolytic activity in an oligotrophic environment (Southern Tyrrhenian Sea, W Mediterranean)

The variations in the expression of two hydrolytic ectoenzymes (leucine aminopeptidase – LA – and β glucosidase — BG) were studied in the southern Tyrrhenian Sea during spring 2004. This area is characterised by a complex morphology and hydrodynamism, which generate significant differences between different sectors, particularly in the 0–100 m layer. However, the area generally exhibits oligotrophic features such as low autotrophic pigment and organic matter concentrations and a higher bacterial biomass than the phytoplanktonic one. Despite this general bottom-up pressure, adaptations by the microbial consumers were indicated by the ectoenzymatic activities and by the relationships between the enzymes, their organic substrates and their producers (namely the bacteria). In particular, bacteria were able to exploit the inorganic N supply (nitrite + nitrate provided by irregular intrusions of intermediate waters) to escape the bottom-up limitation and produce enzymes such as BG devoted to the degradation of cellulose remnants and, therefore, also able to take advantage on this refractory organic matter. In the 200–800 m layer, where trophic limitation was strong due to the low values of potentially-labile organic matter (namely proteins), the peculiar hydrodynamism led to the formation of nepheloid layers rich in organic matter, which provided the bacteria with substrates and allowed the development of a significant correlation between LA activity and its own organic substrate. Furthermore, a reduction of the bottom-up pressure was also indicated by a higher mean bacteria cell size in the entire water column of the central and eastern sectors, and a significantly increased expression of BG related to the increase in the cell size.The ectoenzymatic activities, therefore, suggested that the southern Tyrrhenian Sea should be considered as a mosaic of subsystems, where the peculiar hydrological features stimulate bacterial adaptations and enhance the channelling of energy embedded in refractory materials into the food web.     

站内一体化轨道电路工程应用的探讨

阐述站内一体化轨道电路的由来以及我国高速铁路和客运专线将采用的站内ZPW-2000一体化轨道电路的技术方案；从轨道电路和CTCS-2级列控系统安全性的角度，对站内一体化轨道电路列控信号电流减弱、道岔跳线断线、绝缘节破损、短轨道区段对列控车载设备工作的影响、站内轨道电路电气连接和电缆使用等方面的问题进行了分析，并提出一些解决方案或思路。     

重庆库区松散土体物理力学参数的数理统计分析

岩土物理、力学参数最重要的性质是具有复杂的空间变异性和明显的不确定性 ,笔者将岩土的物理力学参数视为具有随机性和结构性特征的变量入手 ,运用数理统计方法对岩土的物理力学参数进行分析 ,统计结果显示 ,通过控制压实状态试验得到的岩土参数是可靠的     

聚氨酯焦油在二级路水泥砼路面中的应用

全面介绍了聚氨酯焦油填缝料的成份、性能及在二级路水泥砼路面中的施工工艺和施工方法 ,具有一定的参考价值     

血清金属蛋白酶组织抑制因子-1和抑制因子-2在不同肝病中的意义

目的　建立一种改良固相致敏红细胞吸附技术 (SPASE) ,用于快速检测各种肝病患者血清中金属蛋白酶组织抑制因子 1 , 2 (TIMP 1 ,TIMP 2 ) ,了解血清TIMP 1和TIMP 2在各种肝病患者中的意义。方法　用TIMP 1和TIMP 2单克隆抗体分别包被微量血凝板 ,加热冲洗后加入待检血清标本 ,最后加入单克隆抗体致敏红细胞。用致敏红细胞作为指示系统 ,观察红细胞吸附状况来判定结果。结果　SPASE法全过程仅需要 2 5～ 3h ,共检测 6 5 4例肝病患者血清标本 ,TIMP 1和TIMP 2阳性率分别为急性肝炎组 1 7 3 9%、1 4 1 3 % ;慢性肝炎组 3 3 1 9%、2 7 88% ;肝硬化组 82 84%、6 7 1 6 % (P <0 0 0 1 )。结论　TIMP 1和TIMP 2表达的变化可作为肝纤维化较为有用的诊断指标 ,TIMP 1的诊断意义更大。SPASE法具有较高的敏感性、特异性和快速性 ,不需复杂的仪器设备 ,操作简单 ,尤适于医院及基层医疗单位。     

A PRELIMINARY STUDY ON SURVIVIN AND BCL-2 EXPRESSION IN CERVICAL CARCINOMAS

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