尿毒症apoE基因敲除小鼠主动脉动脉粥样硬化的病变特征

目的 观察尿毒症apoE-/-小鼠主动脉粥样硬化(As)病变特征及MMP-9的表达.方法 以apoE-/-小鼠为研究对象,通过2步外科手术法建立尿毒症模型,对照组行假手术.造模后检测肾功能,判断造模是否成功.造模后10周,检测血清MMP-9浓度;主动脉根部切片油红O染色计算斑块相对面积,天狼星红染色计算斑块内胶原含量,α-肌动蛋白、MAC3免疫组化染色检测斑块内SMC及巨噬细胞的含量;RT-PCR法检测主动脉MMP-9 mRNA的表达.结果 造模后肾功能检测显示造模成功.尿毒症组与对照组相比:主动脉根部As斑块相对面积提高、斑块内胶原含量减少、SMC成分减少、巨噬细胞含量增加,血清MMP-9浓度及主动脉MMP-9 mRNA的表达提高.结论 尿毒症apoE-/-小鼠主动脉加速发展的As伴随着斑块的稳定性降低.     

宁夏回族高脂血症患者CYP2C9*3、MSA2756G基因多态性的研究

目的 研究高脂血症治疗的药物相关基因CYP2C9*3、甲硫氨酸合成酶(MSA2756G)在宁夏回族高脂血症患者中的分布及其与高脂血症的关系.方法 通过扩增引进限制性酶切位点(ACRS)和应用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术对高脂血症患者进行基因型分析.结果 180名宁夏回族高脂血症患者中,CYP2C9*3等位基因频率为3.33%,突变频率男性(3.05%)高于女性(0.28%),差异有显著性(P<0.01),而与健康对照组(3.00%)比较,差异无显著性(P>0.05);MSA2756G等位基因频率为15.83%,显著高于健康对照组(10.25%)(P<0.05),未发现性别差异.结论 宁夏回族人群携带CYP2C9*3基因型的男性患高脂血症风险更大;MSA2756G可能是高脂血症的危险因子.     

Association between the -1562 C/T polymorphism in the MMP-9 promoter and phenotype of esophageal squamous cell carcinoma in northern Chinese population

Objective To conduct a case-control study on the association of the nucleotide polymorphisms in the promoter region of the matrix metalloproteinase-9 (MMP-9) gene with phenotype of esophageal cancer. Methods All subjects were unrelated residents in northern regions of China. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was used to determine the MMP-9 genotypes. Results The overall distribution of genotypes in the patients was not different from that in the controls (OR=0.77, 95% CI=0.45-1.34; P=0.36). There were no significant differences between the patients and the control subjects in terms of the distributions of sex and age, smoking status, alcohol dependence, pickled diet status, or history of environmental exposure. The patients were further examined with stratifications by age, sex, grade, depth of tumor invasion, lymphatic invasion, venous invasion and TNM staging. The results showed no pronounced association among the stratifications. Conclusion There is no significant association between the MMP-9 single nucleotide polymorphism genotypes and phenotype of esophageal cancer.     

上海世博会停车换乘交通组织

为实现上海世博交通集约化组织目标、降低自驾游客出行比例,建设世博停车换乘设施并组织引导自驾游客采取P＋R模式前往世博园区最有效措施之一。借鉴日本爱知世博会等大型公共活动停车换乘组织经验,提出上海世博会P＋R交通组织基本思路,将服务对象划分为一日往返自驾游客和本地化自驾游客两类。分析P＋R泊位需求的影响因素,并进行规模预测,得到两类游客的泊位需求总体规模和空间分布。最后,提出分三个层次设置P＋R设施以及完善世博P＋R配套设施和政策的具体建议。     

基于MS9S12DG128单片机的电动汽车电池管理系统设计

根据动力电池组在电动汽车上的使用特点和要求,采用了带MSCAN、且具有强大功能模块的嵌入式微处理器MS9S12DG128,完成了电动汽车用电池管理系统的硬件设计,实现了对电压、电流、温度的实时精确采集、SOC的精确估计和CAN通讯。实验表明,本系统各方面性能优越。     

基于目录的数字证书检索系统的研究

数字证书等数据安全技术已普遍应用于电子商务中,实际上,可以推广到任何涉及身份验证和信任机制的应用领域。目录是网络中包括安全在内的所有资源管理的基础结构,结合了目录服务的数字证书有着更深更广的应用前景。本文试分析一类基于目录的数字证书检索系统,并给出了其实现的基本框架。     

港口竞争力评价模型研究

随着港口间的竞争日益加剧和港口功能的拓展,港口竞争力所涉及的范围也日益变化,在此背景下,对港口竞争力进行研究就显得尤为重要。为了对港口竞争力进行分析,本文针对港口指标多输入多输出的特点,应用数据包络分析方法,分别引入C2R和C2GS2模型,对中国主要港口竞争力进行评价,以期找出非有效性的港口,并对其提出改进建议。作者根据实际港口输入输出数据指标,对10个港口进行了有效性评价,结果表明DEA用于港口竞争力评价是有效的。     

基于X86PC服务器虚拟化解决方案

针对目前铁路部门机房X86 PC服务器数量较多,且资源利用率低下,维护和升级不便等问题,提出了对X86 PC服务器虚拟化解决方案,重点介绍VMware相关产品如何实现虚拟化的方法.     

电动汽车无刷电机控制系统的设计

为对无刷直流电机实现开/闭环两部分控制,文章介绍了基于Freescale 9S12XS128芯片实现对电动汽车无刷直流电机控制器的设计。程序的编译采用了Codewarrior软件,闭环控制即在车上安装了振荡传感器,可根据不同的振荡程度自行调整无刷电机的转速。开环控制是外设有滑动变阻器,用户可以通过调整滑动变阻器来控制输入电压信号的大小,进而调整PWM的输出来控制电机的转速。此款控制器实现了电机的开/闭环控制,还设计有显屏,可显示速度、电流、电压及消耗的电量,且预留有无线输出口并配有LabVIEW上位机软件可用于调试电机和采集数据。     

Environmental innovation and the role of stakeholder collaboration in West Coast port gateways

The paper explores the role of stakeholder collaboration in the adoption of innovations as part of the environmental and sustainability agenda of port gateways. We do this through a comparative assessment of the port of Vancouver, British Columbia, and the twin ports of Los Angeles and Long Beach, California. An inductive research approach is used to identify and assess the initiation and implementation process behind exemplary innovations. Innovation includes new technologies and processes for handling and moving cargo, mechanisms for planning and policy making, as well as financing, implementing, upgrading, managing and operating infrastructure systems. A relatively new arena for competition on the basis of innovation concerns environmental performance. The conceptual framework and empirical evidence suggest that while there may be intense demand for and supply of innovation in port gateways, the complex dynamics of the logistics chain are such that successful innovation requires conscious involvement and collaboration of stakeholders.