CREATE TRANSGENIC RABBITS BY MICROINJECTING HUMAN apoA-ⅡGENE INTO FERTILIZED EGGS

Objective To create transgenic rabbits by microinjecting human apolipoprotein A-Ⅱ (apoA-Ⅱ) gene into one-cell embryos, to study apoA-Ⅱ gene function on plasma lipoprotein metabolism and atherosclerosis. Methods Superovulation and synchronization of estrus were induced in female Japanese White Rabbits by injecting hormone, then mating with male. After collected the fertilized eggs, the human apoA-Ⅱ gene was microinjected into the male pronucleus of eggs. The injected eggs were transferred into recipient female rabbits. Last, extract DNA from the new borns ear and determine whether the newborns were transgenic by polymerase chain reaction (PCR) or Southern blot analysis. Results A total of 822 embryos with microinjection of human apoAⅡ gene were implanted into 28 recipient rabbits. The number of surviving newborns was 37. 3 transgenic positive surviving founders were found with human apoA-Ⅱ.     

基于单总线技术的车顶钥匙管理柜的设计

采用单总线技术设计车顶钥匙管理柜来替代人对车顶钥匙的管理、分配。排除人对车顶钥匙管理的干预，保证车顶钥匙管理工作的安全、可靠。单总线钥匙柜通过USB接口与计算机主机进行通讯。     

Formation of Denmark Strait overflow water and its hydro-chemical composition

The dense overflow across the Denmark Strait is investigated with hydrographic and hydro-chemical data and the water mass composition of the Denmark Strait Overflow Water (DSOW) is determined by multivariate analysis. Hydrographical properties, the transient tracers CFC-11 and CFC-12, oxygen and nutrients are utilized for the water mass definitions. Distribution and characteristics of water masses north of Denmark Strait are described, the important water masses at the sill and the variability on weekly time-scales are discussed, and the entrainment and mixing of water into the overflow plume in the northern Irminger Basin is calculated. The analysis indicates that water masses both from the Nordic Seas and the Arctic Ocean are important for the formation of DSOW. It is found that water masses transported with the East Greenland Current make up about 75% of the overflow at the sill. The overflow at, and shortly south of, the sill is inhomogeneous with a low-salinity component dominated by Polar Intermediate Water. The high-salinity component of the overflow is mainly of Arctic origin. The water mass composition, and the short-term variability for 7 repeats of sections close to the sill are described, and these illustrate that the overflow is in fact a composite of a number of water masses with different formation and transport histories. This indicate that the overflow is a robust feature, but that it responds to variations in the circulation or atmospheric forcing that influences the formation of intermediate and deep water masses within the Arctic Mediterranean and the North Atlantic. At a section about 400 km south of the sill the overflow is well mixed and modified by entrainment of, mainly, Iceland–Scotland Overflow Water and Labrador Sea Water, together constituting 30% of the overflow plume. The entrainment of Middle Irminger Water dominates shortly downstream of the sill, before the overflow plume reaches too deep but the entrainment seems to be intermittent in time.     

厚大顶部活塞模具的改进

以195 t活塞模具为例，研究了在生产厚大顶部活塞毛坯时所遇到的缩孔问题。经过分析得出结论：造成厚大顶部活塞毛坯燃烧室缩孔问题的主要原因是由于模具本身的弊端和水冷系统效果不佳。在此基础上，提出了通过改进水冷底模的设计等方法以加强活塞顶部的冷却效果，促使活塞实现顺序凝固，实践证明，所提出的方法是可行的，为厚大顶部活塞模具的设计提供了一些思路。     

IEC104规约在城轨电力监控系统中的应用

介绍了IEC60870-5-104规约,分析了该规约在城市轨道交通电力监控系统应用中的优势,并结合广州地铁三号线基于IEC60870-5-104规约的变电所通信软件的设计需求,给出了IEC60870-5-104规约在城市轨道交通电力监控系统中应用的典型设计方法。     

南京长江大桥管理处铁路职工生命质量状况评价

目的了解长江大桥铁路职工的生命质量现状及其主要影响因素，为提出改善和防治措施提供依据。方法采用中文版健康调查量表SF-36对不同性别、职业、年龄、工龄职工的生命质量进行测量和评价。结果女性生命质量状况好于男性，旅游公司铁路职工生命质量状况优于管理处，随年龄增长、工龄增加，生命质量呈下降趋势。结论生命质量的影响因素较多，性别、职业、年龄、工龄等都是不可忽视的方面。     

流行性出血热肾素—血管紧张素——醛固酮系统的临床研究

我们在1981年初步观察流行性出血热(EHF)各期血管紧张素Ⅱ(ATⅡ)水平的基础上,于1984年又对肾素—血管紧张素—醛固酮系统(RAAS)各组成分—血管紧张素Ⅰ(ATⅠ)、ATⅡ、醛固酮(ALD)的血浆含量进行了系统观察。54例患者ATⅡ从发热期开始升高,低血压及少尿期达高峰,多尿期后逐渐下降;ATⅠ各期均减低,低血压及少尿期最明显;ALD在低血压及少尿期与ATⅡ相应地增高。以上改变与病情轻重及尿素氮相平行。实验结果进一步证实RAAS与EHF休克及急性肾功能衰竭的发病有重要联系,故早期稳定体液平衡并拮抗RAAS激活及其影响。是EHF治疗的重要关键。     

对完善抗滑桩预算定额的探讨

根据内昆铁路和四川金筠地方铁路山区抗滑桩施工情况,结合施工现场所收集的第一手资料,对抗滑桩预算定额在使用过程中出现的问题和争议较多的地方提出了建议,为进一步完善抗滑桩预算定额提供了可供参考的资料.     

铁路车站进路选择优化模型及求解算法的研究

铁路车站进路选择是车站运输组织的基础,合理地安排车站内各项作业的运行进路及其排列时机有利于提高运输生产效率,节约生产成本。本文以一般技术站为背景,从数学规划的角度研究车站进路的自动选择方法。通过定义衔接点和承载点,建立了车站网络的描述方法。以作业晚点时间最短以及各进路的总走行时间最短为目标,以避免车站作业的时空交叉、满足作业计划要求为约束构建了车站进路选择的数学规划模型。利用进路选择和进路排列时间的映射关系,把进路选择模型转化成一个等价的0-1整数规划模型。针对模型的非线性特点,以模拟退火算法为基础,提出了适合求解进路选择模型的复合优化算法。通过算例验证了模型的正确性以及求解算法的有效性。     

民用建筑三相配电中性线电流的确定

本文引入相量负载系数β_k(k=1,2,3)来描述电路中三相负载的动态变化,通过对常见的三相四线电路的分析,从理论上获得了中性线电流上限值的计算公式。文中的分析方法和结论对配电设计有一定的参考价值。