亚洲牛带绦虫成虫延伸因子-1基因的表达、纯化及免疫学分析

目的 对亚洲牛带绦虫成虫延伸因子-1(elongation factor 1,EF-1)基因进行克隆、表达和免疫学初步研究.方法 将亚洲牛带绦虫成虫EF-1克隆到原核表达质粒pET-28a( )中,在大肠杆菌BL-21/DE3中用异丙硫代-β-D半乳糖苷诱导表达,表达产物通过十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)进行鉴定,用镍离子金属螯合剂亲和层析柱进行纯化,用蛋白印迹法(Western blotting)进行免疫学分析.结果 PCR、双酶切及DNA测序结果 均表明重组质粒pET-28a( )-EF-1构建成功.重组蛋白可被感染了亚洲牛带绦虫患者血清和猪血清识别,表明其具有免疫反应性.结论 亚洲牛带绦虫成虫EF-1基因可在原核表达系统中获得具有免疫学活性的表达,为进一步研究该蛋白的功能奠定了基础.     

转K1型转向架组装工艺

介绍了转K1型转向架的结构特点和关键技术参数,着重分析了侧架导框的加工、侧架与支撑座板的组焊和转向架的组装工艺等。     

灰色线性回归组合模型在地面沉降预测中的应用

选择合适的沉降与时间的关系模型对于沉降预测而言是很重要的。根据地面沉降机理与曲线特征，对某数据序列进行了光滑性和指教规律性检验，使用线性回归和灰色模型的组合模型对该数据序列建模和预测，所得平均相对误差和均方差比值都优于GM（1，1）。研究结果表明，具有线性特征的沉降数据序列选用灰色线性回归组合模型进行短期预测效果更好。     

Dynamics and fishery of the Peruvian hake: Between nature and man

Mean length in the catch of Peruvian hake (Merluccius gayi peruanus) declined drastically by 8 cm in a few months in 1992. The 1991–93 El Niño event marked the beginning of changes in bottom environmental conditions, that along with other changes in the ecosystem, were the cause of the disappearance of large sized old hake from the traditional fishing grounds and the invasion of these areas by high concentrations of small sized young hake, traditionally distributed in the southern areas. A misinterpretation of this change gave an optimistic perception of the state of the stock, leading to a large increase of the fishing fleet and therefore to an increase of the fishing pressure on this resource. Catch increased quickly to very high levels in 1996, the fishery indicators of relative abundance remained stable, while global abundance of hake was diminishing. The resource responded to these natural variations of environment and to the intense fishing pressure with changes in some aspects of its biology and ecology. The most remarkable was the decrease of the length and age at first maturity that led to spawning by 1 year old (19 cm) individuals in a species whose observed longevity was 14 years.In 2002 a total and indefinite fishery closure was established by the Peruvian government and a technical commission for the hake recovery was created, which carried out continuous monitoring of the stock. After a closure of 20 months the indicators showed signs of recovery and the fishery was reopened in 2004. However, after an encouraging beginning during 2004 and the first quarter of 2005, neither abundance nor age structure had improved by the end of 2005, and in fact were deteriorating by the start of 2006. The survival of the age 2 and 3 groups was very low and few 4+ years old individuals were observed. Several factors working at different time scales have been proposed to explain the status of hake. The impact of the fishery, variations in the abiotic environment, predation by the jumbo squid (Dosidicus gigas) and cannibalism were considered as potential causal factors of this decline.     

船坞工程防渗及止水工艺介绍

本文介绍了船坞工程施工中防渗及止水工程的工艺及防渗止水效果分析。经采用多种不同的防渗、止水结构,保证了船坞有良好的防渗、止水效果。     

哈尔滨市城市交通拥堵原因及解决措施

城市交通拥堵问题表象在城市道路交通,其根源受城市的自然环境、气候、历史、规划以及市民岀行习惯等方面影响。全面、客观地分析总结了影响哈尔滨城市交通拥堵的原因,对解决城市交通拥堵问题进行了思考,并提出了建议和具体措施。     

基于“1+X”证书制度的汽车类专业人才培养模式创新研究——以新能源汽车技术专业为例

文章主要是在“1+X”证书制度视角下,以新能源汽车技术专业为例,将“1+X”证书制度运用到汽车类专业人才培养模式中,更有效地提高汽车类专业人才培养质量。基于“1+X”证书的人才培养方案创新是在已有的教学模式基础上,深化教育教学改革,构建实训考核平台,加强教师队伍建设,并在实训过程中重视理论与实践的结合,将学生的理论知识运用到实践中,从而培养真正优秀的新能源汽车专业人才。     

考虑复合地基侧摩阻力作用下土中竖向应力解

为了估算新建路基对既有路基影响,以半无限体内Mindlin应力方程解为计算基础,假设复合地基为一个加固整体,对复合地基侧壁摩阻力进行积分,推导复合地基侧摩阻力在土体中引起的附加应力的解析表达式,计算在复合地基作用下距填筑坡脚处一点的土竖向附加应力。     

未定式“1^∞”型的一个简便方法

本文对重要极限lim x→0（1＋x）1/x的另一种判别法进行论证,然后将此判别法运用到求解极限未定式＂1^∞＂型这类难度较大的题型中去,通过比较,论述,这种判别法给出的计算方法简便易行,从而解决了教学中的一大难点,具有较好的实用性。     

Study on driver model for hybrid truck based on driving simulator experimental results

In this paper, a proposed car-following driver model taking into account some features of both the compensatory and anticipatory model representing the human pedal operation has been verified by driving simulator experiments with several real drivers. The comparison between computer simulations performed by determined model parameters with the experimental results confirm the correctness of this mathematical driver model and identified model parameters. Then the driver model is joined to a hybrid vehicle dynamics model and the moderate car following maneuver simulations with various driver parameters are conducted to investigate influences of driver parameters on vehicle dynamics response and fuel economy. Finally, major driver parameters involved in the longitudinal control of drivers are clarified.