P27~(Kipl)与Ki-67抗原在结直肠腺瘤、腺癌组织中的表达及意义

目的　通过检测P2 7Kipl及Ki 6 7抗原在结 (直 )肠腺瘤、腺癌中的表达 ,探讨其与临床病理指标及预后的关系。方法　采用免疫组织化学方法检测了 2 0例正常结 (直 )肠粘膜、16例腺瘤、6 2例腺癌的P2 7Kipl及Ki 6 7抗原的表达。结果　正常粘膜、腺瘤至腺癌的等级与P2 7Kipl表达等级呈负相关 ,而与Ki 6 7抗原表达等级呈正相关。腺瘤与腺癌细胞质P2 7Kipl表达有显著性差异 (P <0 .0 5 )。腺癌组P2 7Kipl表达与Ki 6 7抗原表达、肿瘤有无转移、Dukes’分期及国际TNM分期呈负相关 ;Ki 6 7抗原表达与有无转移呈正相关。P2 7Kipl表达阳性腺癌患者无病生存时间长于表达阴性者。结论　在结 (直 )肠肿瘤的发生、发展及转移过程中 ,P2 7Kipl表达下调 ,Ki 6 7抗原表达上调 ;P2 7Kipl表达对评估结 (直 )肠癌患者预后有一定帮助。     

基于DS18B20的温度实时采集与显示系统的设计与实现

本文介绍了基于DS18820的温度实时采集与显示系统的设计与实现。设计中选取单片机AT89S52作为系统控制中心,数字温度传感器DS18820作为单片机外部信号源,精度较好的液晶显示模块1602显示温度。在软件方面利用C语言编程控制单片机,完成对DS18B20与AT89S52的控制,最终实现温度的实时采集与显示。     

哈大线电气化铁道远动系统简介

介绍了哈尔滨大连电气化铁道所引进德国的S.P.I.D.E.R.远动系统用于对供电系统的控制、监督、采集和分析数据,并提供一个建立网络控制应用的操作平台。系统的引进为我国电气化铁道远动控制提供了可借鉴的经验。     

Distribution of total dissolved and C18 extractable copper and nickel in relation to dissolved organic matter sources, in the Thermaikos Gulf (eastern Mediterranean)

Organically complexed and total dissolved copper and nickel were measured in the Thermaikos Gulf waters during winter 1998. Total concentrations ranged from 1.13 nmol/L to 7.02 nmol/L for copper and from 3.04 nmol/L to 9.50 nmol/L for nickel. The C18 Sep-Pak solid phase extraction technique was used in order to determine the amount of copper and nickel complexed with the hydrophobic component of the dissolved organic matter (DOM). Up to 32.4% of total copper and 4.57% of total nickel were complexed with the DOM extracted by C18. By applying HPLC analysis it was shown that the majority of the organic compounds retained by C18 had intermediate to low polarity. Using the absorbance ratio a₂₅₄/a₄₃₆ it was possible to unravel that humic substances of both terrestrial and marine origin were present in the Thermaikos Gulf and their distribution followed the dilution of riverine waters. Moreover our results indicated that organic binding of both Cu and Ni was stronger with the hydrophobic compounds of terrestrial origin than with those of marine origin.     

运三高速公路病害路段技术处治

运三高速公路自通车以来，部分路段出现严重的车辙等病害，根据路况调查结果，提出病害路段技术处治措施及维修施工质量控制要点。     

基于GPRS网络的数据无线传输实现接口

介绍了GPRS无线数据通信的工作原理及应用GPRS技术进行无线通信的设计方案、软硬件的实现。嵌入式GPRS无线通信技术为数据传输业务提供了极大的便利,缩短了应用系统的开发周期,减小了开发难度和风险,具有很高的市场应用前景。     

RECAP与PRO-FORMAC/P的条款适用及合同解释问题英国高院“LOWLANDS ORCHID”轮案判例简析

文章主要研究RECAP与PRO-FORMA C/P约定相冲突的情况下,条款的解释与适用,并对如何合理订立租约、避免相应风险提出相关建议。     

T-2毒素中毒对低硒大鼠关节软骨细胞凋亡相关基因mRNA表达的影响

目的探讨T-2毒素对低硒喂养大鼠关节软骨细胞凋亡相关基因mRNA表达的影响,以了解大骨节病病因及软骨细胞凋亡的发生机制。方法新生的雄性大鼠随机分为4组,正常饲料组、低硒饲料组、正常饲料+T-2毒素组、低硒饲料+T-2毒素组。正常饲料和正常饲料+T-2毒素组、低硒饲料组和低硒饲料+T-2毒素组大鼠分别给予人工合成正常饮食和低硒饮食30 d。之后,正常饲料+T-2毒素组和低硒饲料+T-2毒素组给予T-2毒素(每天每克体重200 ng)灌胃30 d。提取大鼠关节软骨RNA,采用Real-Ti me PCR法检测凋亡相关基因P53、caspase-3、Bcl-2和Bax的mRNA表达。结果与正常饮食大鼠血硒(73.92±30.01)ng/mL相比,低硒饮食30 d的大鼠血硒为(4.16±3.56)ng/mL,二者之间有统计学差异(P<0.05)。与正常饲料组比较,低硒饲料组、正常饲料+T-2毒素组、低硒饲料+T-2毒素组中的P53、caspase-3、Bax的mRNA表达上调,Bcl-2的mRNA表达下调,差异有统计学意义(P<0.05);与低硒饲料组相比,低硒饲料+T-2毒素组中的P53、caspase-3、Bax的mRNA表达上调,Bcl-2的mRNA表达下调,差异有统计学意义(P<0.05);P53、caspase-3、Bax的mRNA在低硒饲料+T-2毒素组表达高于正常饲料+T-2毒素组;Bcl-2的mRNA在低硒饲料+T-2毒素组表达低于正常饲料+T-2毒素组,差异有统计学意义(P<0.05)。结论每天每克体重200 ng T-2毒素作用30 d,可以引起低硒饲料喂养大鼠软骨细胞凋亡因子mRNA的表达改变。     

基于S/P值的道路隧道照明光源光谱及相关色温研究

为解决选取隧道照明光源时未考虑光谱能量分布和相关色温因素造成光源选用不当的问题,结合中间视觉模型,对道路隧道常用光源的光谱能量分布和相关色温进行测试研究,分析不同隧道常用光源的光谱能量分布与相关色温关系; 通过引入光谱S/P值的计算方法,从人眼感知角度分析不同隧道常用光源S/P值与相关色温关系。研究表明： 1）LED光源的相关色温随着蓝光比例的增加而增加; 2）不同类型光源的S/P值与光源的相关色温不是一一对应的关系,但LED光源的S/P值随着相关色温的增加而增大; 3）金属卤化物灯、白色荧光灯和LED光源适合应用于隧道照明,但从长远来看,隧道照明选用相关色温较高的LED光源更为合理。     

An investigation into the mechanism of the out-of-round wheels of metro train and its mitigation measures

This paper presents an investigation into the mechanism of polygonal wear of metro train wheels through experiments conducted at field sites. The experiments comprise dynamic behaviour test of vehicle and track system, the wheel transverse wear and polygonal wear measurements, rail corrugation and rail weld joint irregularities’ measurements. Moreover, the numerical modal analysis of the wheelset is also performed. The wheel measurement results show that most wheels exhibit obvious eccentricity and polygonal wear with 5–8 harmonics. The investigation results indicate that the wavelength-fixing mechanism of the wheel out-of-roundness with 5–8 harmonics is the P2 resonance. Four measures have been proposed to mitigate the formation of wheel polygonal wear based on the field measurement results.