Toxicity to the normal hemocytes by ALA-PDT for the ex vivo purging of hematopoietic stem cell grafts

Objective To study the toxic effects of 5-amionlevulinic acid-based photodynamic therapy (ALA-PDT) on human peripheral blood mononuclear cells (PBMCs), cord blood mononuclear cells (CBMCs) and peripheral blood stem cells (PBSCs), and furthermore, to understand the possible causes of this response. Methods We used MTT assay to detect the survival rate of PBMCs, CBMCs and PBSCs after treated by ALA-PDT under the optimum experiment conditions with U937 as control;Annexin V-FITC/PI was used to detect the pattern of cell death induced by ALA-PDT. By using flow cytometry, we detected intracellular PpIX fluorescence intensity. Results After ALA-PDT treatment the survival rate of PBMCs had no significant change;however in PBSCs and CBMCs, the survival rate reduced to 70%, and the survival rate of leukemia cell U937 was the lowest, about 30%. After incubation with ALA,except for PBMCs, intraceUuiar PplX fluorescence intensity of the other two kinds of normal haemocytes and U937 increased obviously. These results combined with the flow cytometry suggested that the main pattern of cell death here was apoptosts. Conclusion Under the optimum experiment conditions, ALA-PDT has a slight effect on normal haemocytes but excellent depletions of leukemia cells. Therefore, it can effectively purify autologons bone marrow or stem cell grafts.     

Relationship between the acid-suppression efficacy of proton pump inhibitors and CYP2C19 genetic polymorphism in patients with peptic ulcer

Obiective To investigate acid-suppression efficacy of proton pump inhibitors(PPls) in relation to CYP2C19 genetic polymorphism on patients with peptic ulcer. Methods By an open, randomized and control trial, fifty nine patients with active peptic ulcer were randomly assigned to receive one of three PPIs on a single dose (20 mg of each drug): omeprazole group (n=19), rabeprazole group (n=20) and esomeprazole group (n=20). lntragastric pH was recorded 1 hour before and 24 hours after administration. CYP2C19 genotype was tested in all patients. Resuits The EMs/PMs ratio of each group was 16/3,17/3 and 17/3, respectively. The total time that intragastric pH＞4, time percent pH＞4 and median pH in PMs patients were significantly higher than those in EMs patients of omeprazole group (P＜0.05). But all these differences were not found in rabeprazole group and esomeprazole group. The pH of nocturnal acid breakthrough (NAB) in both rabeprazole group and esomeprazole group was higher than that of omeprazole group, while there was no significant difference between rabeprazole group and esomeprazole group. Gonclusion The acid-suppression efficacy of omeprazole is highly dependent on CYP2C19 genetic polymorphism, while CYP2C19 genetic polymorphism may have a little influence on the acid-suppression efficacy of rabeprazole and esomeprazole. The acid-suppression action of rabeprazole and esomeprazole is superior to omeprazole, especially on night acid secretion.     

隧限-2隧道通行双层集装箱列车的可行性分析

随着集装箱物流的发展,部分既有铁路要改造为双层集装箱运输通道,然而既有铁路的隧道限界大多为隧限-2,不能满足双层集装箱运输隧道建筑限界。文章通过对限界、接触网悬挂方式及隧道处理措施等方面的研究,提出了隧限-2B隧道通行双层集装箱列车是可行的结论。     

基于气垫效应的二维楔形体入水砰击载荷预报方法研究

本文对二维刚性楔形体入水砰击问题进行研究,将数值模拟与模型试验结果进行对比分析,验证利用数值模拟方法研究入水砰击问题的可行性.获得气垫效应、斜倾角、入水速度对楔形体入水砰击压力峰值的影响规律,并分析了气垫效应对压力峰值的影响机理.最后对砰击载荷预报方法进行研究,获得不同斜倾角、不同速度下楔形体入水砰击压力峰值的预报公式.     

复杂心滩通航河段不同角度碛首坝对航道条件的影响*

长江上游河段多为基岩和卵砾石河床,常见心滩、浅滩及边滩等复杂边界碍航河段。由于工程实际需要及航道整治要求,经常存在采砂坑、开挖航槽、修筑碛坝等改变局部河道边界的情况。以长江上游占碛子河段为例,建立向右偏转0°、5°、10°碛首导流坝数学模型,分析表明:0°碛坝引起的水位壅高流速增幅和分流比变化均较小 ,对占碛子浅滩影响较小;5°碛坝对主槽分流比和航道通航水深及流速的优化更加明显,通航效果更优;10°碛坝对主槽通航条件的改善明显,对流场改变较大,引起了明显的河势演变。为长江上游复杂通航河段航道整治工程的设计和研究提供参考。     

2种SOFC-MGT底层循环系统性能对比分析

设计一种新型固体氧化物燃料电池与微型燃气轮机底层联合循环发电系统结构(SOFC-MGT底层联合循环2),基于Matlab/Simulink软件建立了SOFC-MGT底层联合循环2的仿真模型,并与传统的SOFC-MGT底层联合循环1的系统性能进行对比分析.结果 表明,SOFC-MGT底层联合循环2的电堆输出功率要低于底层...     

泸州港集装箱码头二期工程数学模型研究

在分析长江泸州河段基本特征的基础上,结合定床模型试验提出的集装箱码头二期工程实施方案,采用平面二维水流数学模型进行了水面线和流场分析计算,研究该工程修建后该河段通航条件的变化,结果表明建码头后该河段通航条件基本能满足通航要求.     

永磁直线无刷直流电机推力波动仿真分析及削弱方法

与解析计算的方法相比,基于有限元法的电磁场数值计算在分析电机性能方面的精度更高。据此,本文引入运动电机的时步有限元法,采用APDL程序设计语言,以ANSYS有限元仿真软件为平台,通过建立不同的物理模型,将对直线电机推力波动影响最为显著的齿槽力和边端力分离开来,分别对其进行仿真分析,并根据仿真结果对改型电机的推力波动进行了优化。     

中空纤维膜接触器吸收低浓度CO2气体研究

采用疏水性中空纤维膜接触器为吸收装置,以一乙醇胺(MEA)为吸收液,对低浓度CO2(≤0.5%)进行吸收,考察吸收液浓度、吸收液流量、气体流量和进气CO2浓度对CO2吸收效率η和总体积传质系数KGa的影响规律.结果表明,膜吸收法可以处理浓度为0.1%～0.5%的CO2,且保持较高的吸收效率;吸收过程受气相阻力控制,液相阻力对CO2的传质性能影响不大.根据传质理论,建立传质数学模型,模型结果与试验结果基本一致.     

The Implementation of Mirror-Image Effect in MPEG-2 Compressed Video

Straightforward techniques for spatial domain digital video editing (DVE) of compressed video via decompression and recompression are computationally expensive. In this paper, a novel algorithm was proposed for mirror-image special effect editing in compressed video without full frame decompression and motion estimation.The results show that with the reducing of computational complexity, the quality of edited video in compressed domain is still close to the quality of the edited video in uncompressed domain at the same bit rate.