基于视频的交通事件自动检测技术综述

首先从基于视频的交通事件自动检测技术所涉及的目标提取、车辆跟踪和行为理解等3个步骤中，详细地介绍和比较国际上常用的各种检测算法；其次，从背景建模和车辆阴影两个方面分析了检测算法中存在的技术难点和可能的解决方案；同时，从平均检测时间、检测率和误报率3个方面介绍该技术算法性能的评价方法。最后总结了基于视频的交通事件自动检测的相关技术，展望了该技术的发展前景。     

A structural and empirical model of subsistence activity behavior and income

This paper develops a structural and empirical model of subsistence activity behavior and income. Subsistence activity decisions (work participation and hours of work decisions) and income have an important bearing on activity and travel behavior of individuals. The proposed structural model represents an effort to analyze subsistence activity behavior and income earnings to support a better understanding, and reliable forecasting, of individual travel behavior. The empirical model formulates and estimates an integrated model of employment, hours of work and income which takes account of interdependencies among these choices and their structural relationships with other relevant variables. Social factors that inhibit an individual's employment and work hours decision and affect an individual's income are incorporated in the model. A sample of households from the Dutch National Mobility Panel is used in the empirical analysis.     

硫酸镁对哇巴因诱发豚鼠心脏自发电活动的生理效应

本文采用细胞内固定微电极技术观察了硫酸镁对哇巴因所致的豚鼠离体心室乳头肌自发电活动的影响。所得结果如下:(1)2mM～4mM的硫酸镁可对抗0.4μM因哇巴引起的豚鼠心室肌振荡后电位。(2)2mM～4mM的硫酸镁可抑制0.8um哇酸巴因引起的豚鼠心室孔头肌的自发电活动的频率和4相除极化速度。(3).若用硫酸镁和哇巴因同时灌注则不能诱发心室肌的后电位和自发电活动。提示硫酸镁可预防和治疗哇巴因所致的心律失常。     

对定额基础资料测定的认识与体会

笔者结合参加隧道预算定额基础资料的测定工作,从5个方面介绍了对定额基础资料测定工作的认识及在实际工作中的体会。     

Travel time ratio: the key factor of spatial reach

An important aspect of reach and accessibility is the time people are willing to spend on reaching activity places. In this paper we see the issue of travel time in an alternative way. Instead of looking at travel time separated from time spent on activities, we examine the relation between travel time and stay time. We operationalize this relation with the concept “travel time ratio”. A hypothetical framework underlying these travel time ratios is displayed. We show that for similar types of activity places the value of travel time ratio are in accordance with each other. We find large differences between trips for mandatory activities and trips for discretionary activities. The results indicate the stability of the travel time ratios. Finally, some implications for future research and policy will be mentioned. This revised version was published online in June 2006 with corrections to the Cover Date.     

On the formulation of time-space prisms to model constraints on personal activity-travel engagement

The notion of time-space prisms has often been used in the context of describing activity-travel patterns of individuals. This paper presents a methodology for estimating the temporal vertices of time-space prisms using the stochastic frontier modeling technique. Observed trip starting and ending times are used as dependent variables and socio-economic characteristics and commute characteristics serve as independent variables. The models are found to offer plausible results indicating that temporal vertices of time-space prisms, though unobservable, can be estimated based on temporal characteristics of observed activity-travel patterns. Comparisons of stochastic frontier models of prism vertices and the distributions of prism vertices are presented using two activity data sets collected in the United States – San Francisco and Miami. Differences and similarities in temporal vertex locations are highlighted in the paper.     

DC-CIK细胞的生物学活性及抗淋巴瘤细胞的作用

目的研究细胞因子诱导的杀伤细胞(CIK)与树突状细胞(DC)共培养后DC-CIK细胞的体外增殖能力、免疫表型变化、分泌细胞因子水平以及抗淋巴瘤细胞活性。方法正常人外周血单个核细胞诱导DC和CIK细胞,将DC与CIK共培养,以CIK细胞单独培养为对照。用台盼蓝活细胞计数计算细胞扩增倍数,MTT法测定杀伤活性,流式细胞术分析免疫表型,ELISA双抗体夹心法检测分泌干扰素-γ(IFN-γ)、白细胞介素-12(IL-12)的水平。结果DC-CIK细胞增殖能力明显高于CIK细胞(P<0.05);DC、CIK细胞共培养后,CD3+CD8+、CD3+CD56+双阳性细胞比率较同条件下CIK细胞组显著增多(P<0.05);共培养3 d,DC-CIK细胞上清液中IL-12、INF-γ的分泌量均比CIK细胞单独培养的分泌量高(P<0.01,P<0.05);在5∶1-40∶1的效靶比范围内,DC-CIK细胞对淋巴瘤细胞的杀伤率显著高于CIK细胞(P<0.05),且杀伤率与效靶比呈正相关。结论DC-CIK细胞的增殖能力、分泌细胞因子水平、抗淋巴瘤细胞活性均高于CIK细胞,为DC-CIK细胞免疫治疗提供了实验和理论依据。     

B7.1胞外编码区的真核表达及其生物活性

目的　真核表达B7.1细胞外编码区以研究其生物学活性。方法　电穿孔法将重组质粒pDisplay/B7.1(V +C)导入人子宫颈癌细胞株 ,免疫组化及核酸原位杂交法分别检测其蛋白及mRNA表达 ,3 H TdR掺入法测定表达物对T细胞的刺激作用 ;MTT法研究转染细胞 T细胞混合培养引发的细胞毒性杀伤作用。结果　B7.1细胞外区在Hela细胞表面表达 ,所表达的B7.1细胞外区在抗CD3单抗存在的情况下具有刺激T细胞活化的特性 ,重组质粒转染的Hela细胞与T细胞混合培养可引起细胞毒性杀伤。结论　真核表达的B7.1(V +C)具有T细胞共刺激活性 ,可用于构建肿瘤免疫治疗之双功能分子。