共存递质囊泡循环动力学研究细胞模型的建立

目的直接证明PC12细胞能够合成和分泌组胺(histamine,HA),为将其应用于交感神经共存递质囊泡循环动力学研究提供实验依据。方法常规培养PC12细胞,用嵌套RT-PCR(nRT-PCR)和Western blot技术检测其合成HA的关键酶组氨酸脱羧酶(histidine decarboxylase,HDC)的mRNA和蛋白表达,利用免疫荧光技术观测HA、HDC以及HA囊泡在细胞中的定位。结果从基因和蛋白水平均证实了该细胞中表达HDC,并利用形态学方法在细胞中检测到HA、HDC以及HA囊泡的存在。结论 PC12细胞确实含有HA,结合分化型PC12细胞与交感神经元相似的特征,可将其作为交感神经共存递质囊泡循环动力学研究的理想细胞模型。     

基于供应链的铁路工程项目质量管理体系研究——以京沪高铁济南黄河大桥为例

主要探讨基于供应链环境下的铁路工程项目质量管理体系.以京沪高铁济南黄河大桥工程项目为例,建立了基于供应链的质量策划、质量保证、质量控制和质量评价体系.     

上海港罗泾港区二期工程规划设计创新

新近建成的上海港罗泾港区是集矿石、煤炭、钢铁等散杂货物为一体的综合性港区,是世界上第一座公共港区为大型钢铁企业提供分拨配送等增值服务的物流港区。罗泾港区规划设计开发了全新的"枢纽港-物流港"联动的平行结构平面布置模式。在传统的公用港口布置方法基础上,运用准时制(JIT)的精益生产(LP)物流理念,优化装卸工艺和港口平面布置,提升服务水平,延伸港口服务链条。实现了物流流程的高度一体化、同步化。节约了极为宝贵的深水岸线和土地资源、降低了能耗。     

呼和浩特市汽车4S店发展的探讨

针对内蒙古呼和浩特市汽车4S店的发展及现状,指出目前存在的问题,并提出解决问题的对策与建议。     

Remaining fatigue life assessment of corroded mooring chains using crack growth modelling

Failures caused by the combined actions of fatigue, corrosion and wear are important safety concerns for mooring chains used on floating structures in the oil and gas industry. Prediction of remaining corrosion fatigue life based on surface condition could therefore be a useful tool for the continued safe operation of corroded chains. This paper investigates the use of crack growth modelling for estimating the remaining corrosion fatigue life of mooring chains that exhibit significant pitting corrosion damage. A crack growth modelling approach is used to produce remaining fatigue life estimates for a selection of severely pitted mooring chains. Using fatigue crack growth rate test results for grade R4 high strength mooring chain steel, empirical crack growth laws are presented for free corrosion and cathodic protection conditions at load ratio R = 0.1. Two different methods for establishing equivalent cracks from surface scans of corrosion damage are presented. The mooring chains are proof loaded as part of their manufacturing process. Residual stresses introduced during this process have therefore been determined by finite element analysis and accounted for in the fatigue crack growth predictions. One of the equivalent crack models, accounting for the single dominant corrosion pit, provided quite accurate fatigue life predictions when compared with full scale test results.     

Analysis of S–N data for new and corroded mooring chains at varying mean load levels using a hierarchical linear model

Results from full scale fatigue tests of offshore mooring chains are analyzed. The data set includes new and used chains, tested at a variety of mean load levels. The used chains have been retrieved after operation offshore and include samples with varying surface conditions, ranging from as-new to heavily corroded. Based on a parameterized S–N curve intercept parameter, the effects of mean load and chain condition are estimated empirically by regression analysis. A hierarchical linear model is used, to account for and quantify correlations within subsets of the data. The choice of grouping criterion for the hierarchical model is discussed, and assessed based on the current data. Results show that the mean load and corrosion effects are both significant. Differences in the fatigue performance of new versus used chains are quantified and discussed.     

锚链多体系统最优化求解方法及其对比分析

为了在不添加额外方程或判断条件的情况下，求解锚链多体系统悬链和卧链共存的状态，提出利用最优化原理描述静态锚链多体系统的状态方程，即最小势能方程，并与传统的悬链线方程和多体系统一般力学方程进行对比分析。结果表明，该方法不需要进行复杂的受力分析，形式简单且物理意义明确。数值计算结果与传统方法在计算悬链时是一致的，且可在统一的框架下计算悬链和卧链同时存在的状态。     

Robust adaptive course-keeping control of under-actuated ships with the rudder failure北大核心CSCD

[目的]针对舵机故障、控制增益未知和海洋环境干扰情况下的欠驱动船舶航向保持问题,设计一种考虑舵机故障的船舶鲁棒自适应航向保持控制算法。[方法]通过结合鲁棒神经阻尼技术和自适应方法,对繁重的神经网络权值进行横向压缩,仅需设计2个自适应学习参数对未知增益和舵机故障参数在线补偿,以确保船舶在舵机故障的情况下能够有效执行航向保持任务。通过李雅普诺夫理论,证明所提出的控制器半全局最终一致稳定有界(semi-global uniform and ultimately bounded,SGUUB)。最后以“育鲲”轮为仿真对象,建立非线性Nomoto数学模型,在海洋干扰下进行对比仿真试验验证。[结果]结果表明,在此策略下,“育鲲”轮在舵机故障情况下平均舵角输出比仿真试验中所对比的传统方法降低了51%,可改善航向保持控制效果。[结论]研究结果可为欠驱动船舶的航向保持控制问题提供借鉴。     

车路协同网络中服务功能链可行时长优化映射算法

以车路协同网络为研究对象，针对物理链路间连通时长的差异性，本文提出优化可行时长的服务功能链映射算法。首先，建立车路协同的物理传输网络模型，并分别分析车车链路和车路链路的连通时长；其次，以映射可行时长为优化目标，以服务功能链的映射规则为约束，建立整数规划问题；最后，为求解该NP-hard问题，提出基于改进子图同构的映射算法，联合考虑车路协同环境中的节点属性、链路属性及链路间关联关系，设计剪枝策略，从而实现服务链路在物理链路上的组合优化映射。实验结果表明，通过考虑物理链路连通时长的差异性，并对其进行优化选择，提出算法在车间通信距离150m和最大车速60km·h-1 条件下，可将可行时长提升34.4%；同时，在车车通信范围、车辆数以及最大车辆速度这3项主要参数设置中均可验证发现，所提算法可有效提升映射可行时长。