基于半马尔科夫链的中小堤坝WSN节点能耗研究

无线传感器网络技术的引入,为中小型堤坝监测技术的发展提供了新思路。由于无线传感器网络节点采用电池供电,而堤坝环境中的数据监测条件比较苛刻,电源更换相对困难,无线传感器网络节点能耗的优化在延长整个无线传感器网络生命周期过程中显得尤为重要。本文介绍了无线传感器网络中节点能耗的主要分布,并提出了降低节点能量消耗的策略。借助半马尔可夫链理论建立了节点能耗的数学模型,分析了WSN节点的能耗情况,在保证正常数据通信的情况下,通过对节点状态的管理,延长节点的睡眠时间,从而达到降低整个网络能耗的目的。     

基于物联网的中铁快运物流效益评价指标体系

在对比新旧流程的基础上,从利益相关者的视角出发,用价值链分析理论初步构建指标体系.在问卷调查的基础上,依据问卷调查的数据,用SPSS中的因子分析对指标进行筛选,最终构建了以物流企业、铁路局、顾客、社会影响为准则,38个二级指标的效益评价体系,从而为全面评价和测度中铁快运应用物联网后的物流效益奠定基础.     

供应链控制下的船舶备件管理

从供应链整体角度出发,论述了供应链管理的概念,分析了航运企业备件管理的一般特点、现状和存在的问题,重点阐述了船舶备件在采购、库存、逆向物流及供应商管理等环节下的控制模式以及以船舶备件信息化管理系统为平台的应对不确定性需求的船舶备件库存和采购策略.以浦海航运有限公司船舶备件库存采购管理实践经验为基础,总结我国航运企业在船舶备件管理方面的相关经验,力求提供一种流程高效、信息共享、成本较低、响应敏捷、协同有效的具有核心竞争力的船舶备件供应链模式.     

Cost-benefit of interface management improvement in design-manufacturing chain

Design-manufacturing chain (D-MC) is the most important link of the supply chain with the greatest possibility in cost reduction. How to strengthen the effective management of interfacial relationship between designer and manufacturer and increase the efficency of interface management concerns the efficiency of the whole supply chain. In order to evaluate and improve the impacts of D-MC interfacial relationship, first we should construct the evaluation index system and then we should analyze the total cost and benefit of the interfacial relationship betterment, including the cost of member units in D-MC, the opportunity cost of cooperation partners, and the market returns resulted from the interfacial relationship improvement, which will advance the whole supply chain efficiency as well as enhance the management performance of D-MC. Foundation item: the Chinese Public Service Platform for Science and Technology Management (No. 06DZ22924) and the Economic Globalization and Innovation (No. 08692106700)     

Asymmetric PCR method in generation of HBV ssDNA for pyrosequencing

Objective To explore the optimal primer ratio and concentration of asymmetric polymerase chain reaction (A-PCR) in producing hepatitis B virus (HBV) single-stranded DNA (ssDNA) for pyrosequencing. Methods A-PCR was carried out to generate HBV ssDNA with forward to reverse primers of different ratios (50 : 1, 100 : 1) and concentrations (13. 0 pmol/25μL and 0.14 pmol/25μL, 19. 5 pmol/25μL and 0. 21 pmol/25μL), and the product yield and quality were compared respectively. Results The forward to reverse primer ratio of 50 : 1 provided better yield and concentration of 19. 5 pmol/25μL and 0. 21 pmol//25μL generated a clearer band. Conclusion A simple and feasible method to produce HBV ssDNA for pyrosequencing in batch is established.     

Cloning and sequencing of a DNA fragment encoding N37 apoptotic peptide derived from p53

Objective It was reported that p53 apoptotic peptide (N37) could inhibit p73 gene through being bound with iASPP, which could induce tumor cell apoptosis. To further explore the function of N37, we constructed the cloning plasmid of DNA fragment encoding p53 (N37) apoptotic peptide by using DNA synthesis and molecular biology methods. Methods According to human p53 sequence from the GenBank database, the primer of p53(N37) gene was designed using Primer V7.0 software. The DNA fragment encoding p53 (N37) apoptotic peptide was amplified by using self-complementation polymerase chain reaction (PCR) method and cloned into the pGEM-T Easy vector. The constructed plasmid was confirmed by endonuclease analysis and sequencing. Results The insertion of objective DNA fragment was confirmed by plasmid DNA enzyme spectrum analysis, p53 (N37) gene was successfully synthesized chemically in vitro. The sequencing result of positive clone was completely identical to the human p53(N37) sequence in GenBank using BLAST software (http://www. ncbi. him. nih. gov/cgi-bin /BLASTn). Conclusion The cloning of DNA fragment encoding p53(N37) apoptotic peptide was constructed by using DNA synthesis and pGEM-T Easy cloning methods. With the constructed plasmid, we could further investigate the function of N37 peptide.     

高速列车座椅舒适度优化

为了提升高速列车乘坐舒适性,对CRH2犁高速列车座椅舒适度进行了问卷调查.根据调查结果、中国人体数据、座椅设计理论和人机工程学,用多元回归分析方法,确定了影响座椅舒适度的因素.提出了适合中国人体的座椅尺寸:靠背倾度100°～120°,椅背宽度不大于415 mm,椅背高度不低于729 mmm,头靠应距离座椅面?骀 700～859 mm.     

中国新疆锡伯族3个STR位点的遗传多态性研究

目的　通过对新疆伊犁地区锡伯族人群D1 6S5 3 9,D7S82 0 ,D1 3S3 1 7三个STR位点的基因型及等位片段频率分布的研究和数据统计分析 ,初步探讨其在遗传学研究及法医学应用中的意义。方法　随机抽取 1 1 0名新疆锡伯族无关个体静脉血 ,用柠檬酸钠抗凝冻存 ,采用全血基因组DNA纯化系统提取DNA ,并用复合PCR技术 ,同时扩增上述三个STR位点 ,聚丙烯酰胺凝胶垂直电泳分型 ,银染显色。结果　上述三个STR位点均符合Hardy Weinberg平衡定律。通过种族间比较 ,新疆锡伯族除D1 6S5 3 9位点与北京汉族人无差异 ,D7S82 0位点与西班牙人无差异外 ,其余位点与高加索人、美国黑人、西班牙人和北京汉族人均有明显差异。新疆锡伯族上述三位点累积排除概率为 0 90 76 ,多态性信息总量为 0 9876。D1 6S5 3 9、D7S82 0和D1 3S3 1 7三个位点杂合度分别为 0 90 2 8、0 8875和 0 92 86 ,所有位点杂合度均高于其他种族。结论　上述三个STR位点的综合检验可用于群体遗传学研究和法医学应用。     

人脑源性神经营养因子基因克隆及序列分析

目的　克隆人脑源性神经营养因子 (hBDNF)基因并进行序列分析。方法　提取健康成人末梢血白细胞基因组DNA作为模板 ,应用PCR技术和T 载体克隆法克隆hBDNF基因 ,筛选阳性克隆、酶切鉴定 ,并进行序列测定和分析。结果　DNA序列测定的结果与GenBank提供的已知序列 (M6 1 1 81 )比较 ,所克隆的hBDNF基因从起始密码子ATG到终止密码子TAG全长共744bp ,序列完全相同。结论　自人基因组DNA中克隆hBDNF基因 ,为进一步开展阿尔茨海默病 (AD)的基因治疗积累了资料。     

DETECTION OF HPVB19 IN SERA OF KASHIN-BECK DISEASE PATIENTS

ＴｈｅＰａｒｖｏｖｉｒｉｄａｅａｒｅａｆａｍｉｌｙｏｆｓｍａｌｌＤＮＡｖｉｒｕｓｅｓｗｉｔｈｓｉｎｇｌｅ ｓｔｒａｎｄｅｄ ,ｌｉｎｅａｒｇｅｎｏｍｅｓａｐｐｒｏｘｉ ｍａｔｅｌｙ 5 0 0 0ｎｕｃｌｅｏｔｉｄｅｓ(ｎｔ)ｉｎｌｅｎｇｔｈ .Ｔｈｅｙｉｎｆｅｃｔａｖａｒｉｅｔｙｏｆｈｏｓｔｓ ,ｉｎｃｌｕｄｉｎｇｉｎｖｅｒｔｅｂｒａｔｅｓ ,ｂｉｒｄｓａｎｄｍａｍｍａｌｓ ,ｂｕｔｏｎｌｙｐａｒｖｏｖｉｒｕｓＢ19ｉｓｐａｔｈｏｇｅｎｉｃｔｏｈｕ ｍａｎｂｅｉｎｇ .Ｔｈｅａｇｅｎｔｗａｓｆｉｒｓｔｒｅｃｏｇｎｉｚｅ…