改性多孔聚偏氟乙烯凝胶聚合物电解质膜的制备与性能分析

通过分散聚合制备了丙烯腈-甲基丙烯酸聚乙二醇单甲醚酯共聚物(Poly(AN-co-PEGMEMA)),并将其共混聚偏氟乙烯PVdF(Poylinylidene Fluioride)以制备改性多孔PVdF凝胶聚合物电解质膜.通过扫描电镜观察多孔膜外观,膜的孔径约在1.0~5.0μm之间,这有利于液体电解质的进入;此外,由于共混物极性较高使多孔膜有较好液体电解质溶涨能力.最终多孔凝胶聚合物电解质室温离子导电率较高,可达10-3S.cm-1数量级,其电化学稳定窗口超过4.6 V,这些性能都表明其在聚合物锂离子电池的开发中具有较好的应用潜能.     

苯系物溶解度和分配系数的测定与估算

采用气相色谱的分析技术,用摇瓶法分别测定了苯,甲苯,乙苯的溶解 度（Sw)和分配系数（KOW）。结果表明,溶解度和分配系数之间存在线性关系：lnKow=0.4558-0.9176lnSw。并且运用结构法和摩尔体积法估算了苯及其简单同系物的溶解度和分配系数,估算结果与实验结果能较好吻合,说明估算法是一种简便、可靠的方法。     

Improvement of Two-Dimensional Gel Electrophoresis for Study of Corm Formation Related Proteins in vitro from Taro (Colocasia esculenta)

It is an important step in understanding a bio-logical activity to profile proteins and peptides incells and tissues[1]. Two-dimensional polyacry-lamide gel electrophoresis (2D-PAGE) is very use-ful method for separating complex protein mix-tures[2]. Because of high resolution, improved re-producibility of 2D-PAGE with immobilized pHgradients, it has become one of the standard proce-dures for proteome research. Proteomic analyses are now emerged in manyareas of plant biology, such as genet…     

肝细胞培养液液面乙烷测定与脂质过氧化作用

建立了乙烷气相色谱测定法,并用该法检测了低硒大鼠胎肝细胞及肝组织体外培养液液面气体乙烷的含量,同时与加硒组作了比较。结果有显著差异,提示乙烷的含量是代表脂质过氧化的良好指标,有较高的推广应用价值。     

Simultaneous determination of -2 wtexm-2 -O-glucostde, vitexm-2 -O-rhamnoslde, turin, vitexin and hyperoside by HPLC

A simple, precise, and rapid high-performance liquid chromatographic method was developed and validated for the simultaneous determination of vitexin-2＂-O-glucoside, vitexin-2＂-O-rhamnoside, rutin, vit...     

Sensitive determination of 4-O-methylhonokiol in rabbit plasma by high performance liquid chromatography and application

A novel high performance liquid chromatographic method was developed for the determination of 4-O- methylhonokiol in rabbit plasma and was applied to its pharmacokinetic investigation. Plasma samples were treated by one-fold volume of methanol and acetoni     

空气中四氯乙烯测定方法的研究

本文试用活性炭管采集车间空气中的四氯乙烯蒸汽，用热解吸方法将活性炭管上吸附的四氯乙烯蒸汽解吸下来，用气相色谱法分析样品。分别进行热解吸温度选择，采样管放置稳定性、穿透容量等实验。方法的检测限为１．１×１０－３μｇ／ｍｌ。     

丹参乙酸镁活性代谢产物在大鼠体内的药代动力学特征

目的研究丹参乙酸镁代谢产物M1(3-′O-monomethyl-lithospermic acid B)和M2(3,′3′-′O-dimethyl-lithosper-mic acid B)在大鼠体内的药代动力学特征。方法大鼠静脉注射丹参乙酸镁代谢产物M1或M2,液质联用法进行MRM扫描分析,测定代谢物M1和M2血药浓度,计算药代动力学参数。结果采用该液质联用色谱测定血浆样品中丹参乙酸镁代谢物的方法,血浆内源性物质均不干扰样品峰,相对回收率为:87%-101.4%,日间和日内相对变异系数(CV%)均小于9.77%,血浆中的最低定量限为1μg/L(S/N≥3),线性范围为16-4 096μg/L。M1和M2的t1/2z分别为(1.54±0.81)h和(1.52±0.42)h,MRT(0-t)分别为(0.46±0.07)h和(0.33±0.05)h,AUC(0-tn)分别为(13.63±2.7)(mg.h)/L和(14.96±2.54)(mg.h)/L。结论M1和M2静脉注射的体内代谢过程符合二室模型,消除较快。     

人肺腺癌细胞系A549细胞双向电泳-飞行时间质谱研究

目的初步分析人肺腺癌细胞系A549细胞蛋白质表达情况,从蛋白组水平探讨肺腺癌发病的分子机制。方法应用2-DE技术对人肺腺癌细胞系A549细胞总蛋白进行分离,获得蛋白质表达谱;应用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)结合生物信息学进行蛋白质鉴定。结果3块胶平均蛋白斑点数为1 138±49,平均匹配点数为986±32,匹配率为85.8%。随机选取背景清晰、分辨清楚、蛋白含量较高的20个斑点进行MALDI-TOF-MS分析,共获得了18个肽质量指纹图谱(PMF)。将PMFs质量数据通过Aldente软件查询SWISS-PROT数据库,根据匹配片段及氨基酸序列覆盖率等,初步鉴定出15种蛋白质。根据功能,这些蛋白质可分为:①基本代谢相关的酶类:果糖2-磷酸醛缩酶、视网醛脱氢酶1(RALDH1)、吡多醛激酶;②细胞骨架类:蛋白细胞角蛋白8(CK8)、-β2链微管蛋白;③应激相关蛋白:蛋白二硫化物异构酶A3(PDIA3);④信号转导分子:膜联蛋白1(ANX1)、膜联蛋白4(ANX4);⑤分子伴侣:热休克蛋白60(HSP60)、热休克蛋白-β1、抑制素(PHB);⑥转录及翻译相关蛋白:不均一性核糖核蛋白H(hnRNP H);⑦杂类:Rgr protein、NPM、PCBP1。结论应用2-DE/MALDI-MS方法获得了较为理想的人肺腺癌细胞系A549细胞2-DE蛋白质表达谱,初步鉴定了15种蛋白质。     

Morphology of Polyvinylidene Fluoride Based Gel Polymer Electrolytes

IntroductionDue to their potential application in lithium-ion secondary batteries[1] and other electrochemicaldevices[2 ] ,gel polymer electrolytes have attractedmuch interest in recent years.There were manykinds of polymeric materials being developed aspolymeric hosts for gelelectrolytes. Polyvinylidenefluoride( PVDF) is one of three kinds of matrixthat can be commercially applied[3 ,4] ,and the oth-ers are polyacrylonitrile ( PAN) and poly( methylmethacrylate) ( PMMA) .There were many r…