脂质过氧化对培养人胚肝细胞的损伤作用

利用四氯化碳造成原代培养人胚肝细胞脂质过氧化损伤模型，发现随培养液中丙二醛含量增加，肝细胞在活率下降，谷丙转氨酶释放增多。同时受损肝细胞白蛋白分泌及胞质膜流动性下降。提示脂质过氧化可引起人胚肝细胞多方面损害。     

应用组织芯片和ISH技术检测人体多种肿瘤中hTER mRNA和hTERT mRNA的表达

目的　探讨组织芯片技术在原位杂交中的应用及hTER和hTERT在人体多种常见肿瘤中的表达。方法　利用自行研制的专用器具制作组织芯片 ,采用原位杂交技术 (ISH)检测 2 3 0例人体肿瘤和正常组织中hTER和hTERT基因的表达。结果　①组织芯片中组织样本的组织学结构完整 ,满足病理学实验要求 ;采用组织芯片技术消耗的实验材料、试剂为普通方法的1 /2 3。②在人体正常组织和良性肿瘤、恶性肿瘤组织中 ,hTER和hTERT的表达与肿瘤的性质明显相关 (P <0 .0 0 5 )。结论　组织芯片技术提供了一种快速、有效的组织学方法 ,可广泛应用于原位杂交实验 ,并有可能在肿瘤生物学研究中发挥重要的作用。同时 ,hTER和hTERT表达上调有可能作为人体肿瘤诊断与鉴别诊断的重要组织学指标。     

CH3杆式小量程应变天平的研制

将应变天平用于站台人体模型测力，并为此研制了人体五分量天平。文给出了设计原理，静校公式及动校与实测结果，表明设计合理，可用于相应的试验研究。     

体外观察紫杉醇对人胆管癌细胞QBC939凋亡的诱导作用

目的观察微管稳定剂紫杉醇对胆管癌QBC939细胞的作用。方法MTT法检测紫杉醇对QBC939细胞的抑制作用;光镜和电镜观察紫杉醇作用后细胞形态的变化;流式细胞仪分析细胞周期的改变。结果随着药物作用时间的延长及浓度的增加,紫杉醇对QBC939细胞的增殖抑制作用就越明显。形态学及流式细胞仪分析显示S期及G2/M期阻滞和细胞凋亡。结论紫杉醇诱发的人胆管癌细胞的凋亡与细胞DNA合成期及分裂期阻滞密切相关。这一作用机制将为临床治疗提供理论依据。     

硒对离体人胚肝细胞膜的保护作用

在原代培养人胚肝细胞脂质过氧化损伤模型上，观察硒对肝细胞膜流动性及膜表面超微结构的影响。结果：预加硒可有效地维持肝细胞膜流动性，保护膜表面结构完整。硒保护组培养液中谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）活性明显增加，丙二醛（ＭＤＡ）水平下降，与损伤组比较有显著性差异（Ｐ＜０．０１）。且其ＧＳＨ－Ｐｘ／ＭＤＡ比值与膜流动性增加呈正相关。提示上述硒的保护作用与增强细胞抗氧化能力有关。     

罪犯劳动报酬权初探

劳动报酬权是劳动者基于履行劳动义务而获得的不受非法限制和剥夺的权利。我国罪犯的劳动报酬权是受到限制或剥夺的,它违反了人权保障的现代基本法治理念,违背了我国宪法的有关规定,不符合权利和义务的一致性原则。从促进犯罪分子改造、维护市场交易的平等等方面来说,应当承认和确立服罪犯的劳动报酬权。要建立公正合理的劳动报酬制度,必须确立合理的劳动报酬标准,将罪犯劳动改造的范围限制在社会公益事业里,并对劳动报酬进行合理的分配。     

Influence of Trampling Intensity versus Hydration State on Loss of Biomass from the Intertidal Rockweed,Fucus gardneri

I investigated the impact of human trampling on Fucus gardneri populations at Cattle Point on San Juan Island, Washington. I hypothesized that damage to Fucus fronds would differ depending on hydration state of the plants, number of trampling steps, and time. Over a two-day period, two levels of trampling treatment, 100- and 200-steps, were applied to 18 plots on two rocky intertidal benches. Detached biomass resulting from trampling was collected each day, weighed to the nearest 0.1 gram, re-hydrated overnight in seawater, and re-weighed in order to calculate biomass removed as a function of mean percent Fucus within each plot. Damage to plants did not differ with the hydration state of Fucus. However, loss of biomass did increase with treatment level, with 222 g of Fucus fronds removed from one 100-step plot up to 1302 g removed from a 200-step plot. Percent cover decreased differentially with treatment level, ranging from 10 percent cover in a 100-step plot up to 85 percent loss in a 200-step plot. To determine the effect of hydration on loss of Fucus biomass, future studies should include plants with a higher degree of desiccation than those in this study, more samples to increase the power of the analysis, and a longer period to detect changes due to time.     

An investigation on motor-driven power steering-based crosswind disturbance compensation for the reduction of driver steering effort

This paper describes a lateral disturbance compensation algorithm for an application to a motor-driven power steering (MDPS)-based driver assistant system. The lateral disturbance including wind force and lateral load transfer by bank angle reduces the driver's steering refinement and at the same time increases the possibility of an accident. A lateral disturbance compensation algorithm is designed to determine the motor overlay torque of an MDPS system for reducing the manoeuvreing effort of a human driver under lateral disturbance. Motor overlay torque for the compensation of driver's steering torque induced by the lateral disturbance consists of human torque feedback and feedforward torque. Vehicle–driver system dynamics have been investigated using a combined dynamic model which consists of a vehicle dynamic model, driver steering dynamic model and lateral disturbance model. The human torque feedback input has been designed via the investigation of the vehicle–driver system dynamics. Feedforward input torque is calculated to compensate additional tyre self-aligning torque from an estimated lateral disturbance. The proposed compensation algorithm has been implemented on a developed driver model which represents the driver's manoeuvreing characteristics under the lateral disturbance. The developed driver model has been validated with test data via a driving simulator in a crosswind condition. Human-in-the-loop simulations with a full-scale driving simulator on a virtual test track have been conducted to investigate the real-time performance of the proposed lateral disturbance compensation algorithm. It has been shown from simulation studies and human-in-the-loop simulation results that the driver's manoeuvreing effort and a lateral deviation of the vehicle under the lateral disturbance can be significantly reduced via the lateral disturbance compensation algorithm.     

城市交通工程设计中应体现的人文关怀

在介绍我国城市交通发展现状及未来趋势的基础上,通过分析现阶段城市交通工程设计中存在的问题,探讨了在城市交通工程设计中应体现的"人文关怀"。     

增殖抑制基因和血管内皮生长因子在胃癌中的表达及其意义

目的探讨增殖抑制基因(HSG)以及血管内皮生长因子(VEGF)在胃癌中的表达及其意义。方法采用免疫组化法检测HSG和VEGF在胃正常黏膜、癌旁组织及胃癌组织中的表达。结果胃正常黏膜、癌旁组织及胃癌组织HSG的阳性表达率分别为66.7%、58.0%和38.3%,正常黏膜组到胃癌组HSG表达率呈递减趋势,差异有统计学意义(P<0.05);VEGF在3组中的阳性表达率分别为13.3%、20.0%和68.3%,正常黏膜组到胃癌组的表达率呈递增趋势,差异有统计学意义(P<0.05);Spearman等级相关分析显示,HSG与VEGF表达呈负相关(r=-0.847,P<0.05)。结论 HSG与VEGF的过表达可能是胃癌发生发展以及转移的重要原因之一。