排序方式: 共有75条查询结果,搜索用时 0 毫秒
21.
Xiao-quan Li Shu-lin Zhang Li-hua Zhong Jun Cheng Yuan Hong Meng-dong Lan Xiao-bin Chen Cheng-fu Sun 《西安交通大学学报(英文版)》2009,21(2):99-103
Objective To prepare polyclonal antibody of transactivated protein 5 of hepatitis C virus nonstructural 5A (NA5ATP5) and to explore its expression in the liver tissues. Methods In Escherichia coil BL21, the prokaryotic expression vector pET32a(+)-NS5ATP5 was induced by isopropyl-β-D-thiogalactoside (IPTG), and it was analyzed with sodium dodecyl sulfate-polyaerylamide gel electrophoresis (SDS-PAGE) and Western blotting. And the purified protein was used to immunize the rabbit to prepare polyelonai antibody, with which we studied the function of NSSATP5 by determining the different liver tissues with the streptavidin-perosidase (SP) immunohistochemistry method. Results Recombinant NS5ATP5 (molecular weight: 65 kD) and polyclonal antibody were successfully prepared. NS5ATP5 expression in the liver of patients with chronic HCV infection was much higher than that of a normal person, and it was detected mainly in the cytoplasm. Conclusion The findings of the expression difference between HCV patients and normal people led to a novel diagnostic marker to detect HCV infection. 相似文献
22.
利用原子转移自由基聚合(ATRP)技术合成A2BA2型两亲性液晶嵌段共聚物.聚乙二醇与2,2-二氯乙酰氯反应生成结构对称的多官能团大分子引发剂,引发偶氮苯单体6-[4-(4-乙氧基苯基偶氮)酚氧基]己基甲基丙烯酸酯(AZO6Et)的ATRP反应.利用凝胶色谱(GPC)与核磁共振氢谱(1H NMR)表征了产物的分子量与结构组成,利用示差扫描量热法(DSC)与偏光显微镜(POM)对聚合物的液晶性进行了表征.该液晶嵌段共聚物在光信息储存、光开关与分子器件等领域具有潜在应用. 相似文献
23.
二甲基二烯丙基氯化铵-丙烯酰胺共聚物的研究 总被引:7,自引:0,他引:7
常青 《兰州交通大学学报》2000,19(4):48-50
在分子设计的基础上,采用补加活泼单体制得二甲基二烯丙基氯化铵-丙烯酰胺共聚物,其特性粘度[η]=2.76,阳离子度=23.8%。絮凝实验证明,该聚合物作为絮凝剂特别适用于高浊度水的处理,当与聚合 硫酸复配使用时,效果更为理想。 相似文献
24.
25.
Various polysulfonamide(PSA) statistical copolymers were synthesized by polycondensation of three aromatic diamines and terephthaloyl chloride.The inherent viscosities of the resulting PSA copolymers range from 1.54 to 1.66 dL/g.The precipitated PSA copolymers with feed content of p-phenylene diamine(PPD) being less than 50%(mole fraction) can be redissolved in N-methyl-2-pryrrolidone(NMP) or N,N-dimethylacetamide(DMAc) with lithium chloride of wLiCl=0.02.All of the PSA copolymers exhibit enhanced tensile s... 相似文献
26.
通过采用HX嵌段共聚物改性沥青混凝土填充型伸缩装置在清龙线洲边桥伸缩缝改善工程中的实施,充分了解新型伸缩装置的机理和特性以及技术的可行性。该装置在经济上是合理的,适宜在中小桥伸缩缝改造中推广应用。 相似文献
27.
29.
30.
以L-丙交酯和蚕蛹蛋白为原料,在辛酸亚锡的催化作用下,采用熔融聚合法合成了聚乳酸和蚕蛹蛋白的共聚物(PLLA-co-SCP),研究了投料比对共聚物特性粘数的影响,结果表明:L-丙交酯与蚕蛹蛋白投料比为34∶1时得到特性粘数最大为0.2446 dL/g的共聚物.用傅里叶红外光谱(FTIR)、热重分析(TGA)、差示扫描量热法(DSC)和 X 射线衍射(XRD)对PLLA-co-SCP共聚物的结构、热性能及结晶性能进行了表征.FTIR谱图分析结果表明:合成的PLLA-co-SCP共聚酯兼具了PLLA链段的酯基和蚕蛹蛋白的酰胺基;TGA测试结果显示PLLA-co-SCP特性粘数越大热稳定性越高, SCP含量越高热分解残留率越大;DSC和XRD结果表明PLLA-co-SCP结晶主要由PLLA链段产生,聚乳酸链段越长共聚物结晶度越高,晶体也越完善.另外,XRD结果还表明PLLA-co-SCP共聚物含PLLA 的α和β两种晶体. 相似文献