Polyclonal antibody preparation and expression in liver tissues of transactivated protein 5 of hepatitis C virus nonstructural 5A

Objective To prepare polyclonal antibody of transactivated protein 5 of hepatitis C virus nonstructural 5A (NA5ATP5) and to explore its expression in the liver tissues. Methods In Escherichia coil BL21, the prokaryotic expression vector pET32a(+)-NS5ATP5 was induced by isopropyl-β-D-thiogalactoside (IPTG), and it was analyzed with sodium dodecyl sulfate-polyaerylamide gel electrophoresis (SDS-PAGE) and Western blotting. And the purified protein was used to immunize the rabbit to prepare polyelonai antibody, with which we studied the function of NSSATP5 by determining the different liver tissues with the streptavidin-perosidase (SP) immunohistochemistry method. Results Recombinant NS5ATP5 (molecular weight: 65 kD) and polyclonal antibody were successfully prepared. NS5ATP5 expression in the liver of patients with chronic HCV infection was much higher than that of a normal person, and it was detected mainly in the cytoplasm. Conclusion The findings of the expression difference between HCV patients and normal people led to a novel diagnostic marker to detect HCV infection.     

A2BA2型两亲性液晶嵌段共聚物的合成与表征

利用原子转移自由基聚合(ATRP)技术合成A2BA2型两亲性液晶嵌段共聚物.聚乙二醇与2,2-二氯乙酰氯反应生成结构对称的多官能团大分子引发剂,引发偶氮苯单体6-[4-(4-乙氧基苯基偶氮)酚氧基]己基甲基丙烯酸酯(AZO6Et)的ATRP反应.利用凝胶色谱(GPC)与核磁共振氢谱(1H NMR)表征了产物的分子量与结构组成,利用示差扫描量热法(DSC)与偏光显微镜(POM)对聚合物的液晶性进行了表征.该液晶嵌段共聚物在光信息储存、光开关与分子器件等领域具有潜在应用.     

二甲基二烯丙基氯化铵-丙烯酰胺共聚物的研究

在分子设计的基础上,采用补加活泼单体制得二甲基二烯丙基氯化铵－丙烯酰胺共聚物,其特性粘度[η]＝2．76,阳离子度＝23．8％。絮凝实验证明,该聚合物作为絮凝剂特别适用于高浊度水的处理,当与聚合 硫酸复配使用时,效果更为理想。     

塑性丁苯橡胶SBS

SBS（热塑性丁苯橡胶）是以丁二烯、苯乙烯为单体，采用阴离子聚合制得的嵌段共聚物。 SBS兼有橡胶和塑料的优良性能．具备良好的弹性、耐低温性、透气性、抗温滑性、着色性、电器绝缘性，加工时不需硫化，可直接采用注塑、模压、挤出等多种加工方法成型。     

Preparation and characterization of polysulfonamide statistical copolymers from three aromatic diamines

Various polysulfonamide(PSA) statistical copolymers were synthesized by polycondensation of three aromatic diamines and terephthaloyl chloride.The inherent viscosities of the resulting PSA copolymers range from 1.54 to 1.66 dL/g.The precipitated PSA copolymers with feed content of p-phenylene diamine(PPD) being less than 50%(mole fraction) can be redissolved in N-methyl-2-pryrrolidone(NMP) or N,N-dimethylacetamide(DMAc) with lithium chloride of wLiCl=0.02.All of the PSA copolymers exhibit enhanced tensile s...     

一种填充型伸缩装置在伸缩缝改善工程中的应用

通过采用HX嵌段共聚物改性沥青混凝土填充型伸缩装置在清龙线洲边桥伸缩缝改善工程中的实施,充分了解新型伸缩装置的机理和特性以及技术的可行性。该装置在经济上是合理的,适宜在中小桥伸缩缝改造中推广应用。     

含蜡原油的降凝剂研究

国内原油（包括海洋油田的原油）大都是含蜡黏稠原油和重质高黏易凝原油,这两种类型的原油流动性能差。采用添加降凝剂的方法是改善含蜡原油低温流变特性的途径之一。文中重点介绍了AM（丙烯酰胺）／AA（丙烯酸）／AE（烷基酚聚氧乙烯醚丙烯酸酯）共聚物型降凝剂的性能,系统研究了聚合体系单体质量分数和反应温度对共聚物特性黏数的影响,讨论了共聚物含量对含蜡原油流动性的影响,最后得出添加降凝剂的方法对含蜡原油的输送起到重要作用的结论。     

SBS改性沥青

SBS（热塑性丁苯橡胶）是以丁二烯、苯乙烯为单体，采用阴离子聚合制得的嵌段共聚物。     

聚丙烯酰胺对混凝土抗折强度和弯曲韧性的影响

研究聚丙烯酰胺对混凝土力学性能的改性效果。结果表明聚丙烯酰胺对提高抗折强度、粘结强度和弯曲韧性有显著作用。并可降低压折比。     

L-丙交酯与蚕蛹蛋白共聚物的合成与表征

以L-丙交酯和蚕蛹蛋白为原料,在辛酸亚锡的催化作用下,采用熔融聚合法合成了聚乳酸和蚕蛹蛋白的共聚物（PLLA-co-SCP）,研究了投料比对共聚物特性粘数的影响,结果表明：L-丙交酯与蚕蛹蛋白投料比为34∶1时得到特性粘数最大为0.2446 dL/g的共聚物．用傅里叶红外光谱（FTIR）、热重分析（TGA）、差示扫描量热法（DSC）和 X 射线衍射（XRD）对PLLA-co-SCP共聚物的结构、热性能及结晶性能进行了表征．FTIR谱图分析结果表明：合成的PLLA-co-SCP共聚酯兼具了PLLA链段的酯基和蚕蛹蛋白的酰胺基;TGA测试结果显示PLLA-co-SCP特性粘数越大热稳定性越高, SCP含量越高热分解残留率越大;DSC和XRD结果表明PLLA-co-SCP结晶主要由PLLA链段产生,聚乳酸链段越长共聚物结晶度越高,晶体也越完善．另外,XRD结果还表明PLLA-co-SCP共聚物含PLLA 的α和β两种晶体．