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近日,上海交通执法总队和警方在各交通枢纽开展“整治非法营运,打击克隆出租”整治行动,一批假冒正规出租车的“李鬼”黑车在信息网络技术的手段下被打回原形。 相似文献
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粒子群优化算法中粒子更新方法研究 总被引:3,自引:1,他引:2
粒子群优化算法是根据鸟或鱼群居社会行为而提出的随机优化算法,但标准粒子群优化算法存在早熟收敛和搜索精度低等问题.因此模拟生物克隆选择中5%的B细胞自然消亡过程,在粒子群优化算法进化过程中分别基于代间差分、混沌理论、变异原理等方法设计了8种粒子更新算法,并按照模拟退火方法进行更新后粒子的选择.通过数值实验得出基于代间差分和混沌变异的粒子更新算法(即算法8)是一种很好的选择,并且当性能较差的20%左右粒子按照这种算法更新时效果较好.这种算法可以有效克服标准粒子群算法的早熟现象,并能够加快收敛速度. 相似文献
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To obtain the recombination protein of renalase and prepare the monoclonal antibody, the human renalase gene was amplified
from human kidney by specific primer and cloned the DNA fragments into the pET22b. After verification of the positive clones,
the gene was transformed to E. coli BL21 to express the protein with 6His on C terminal. The Balb/c mouse was immunized with the purified protein to prepare
the monoclonal antibody by hybidoma technique. The renalase protein was reconstructed and 2 strains of the hybidoma which
can stable secrete renalase were obtained. The monoclonal antibody can both react with the both recombinant and human serum
renalase.
Foundation item: the Scientific Research Project of Shanghai Municipal Health Bureau (2008Y034), and the Natural Scientific Research Project
of Shanghai (05ZR14086) 相似文献
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