用聚合酶链反应检测口腔癌组织中人乳头状瘤病毒的E_6转化基因

作者采用聚合酶链反应（ＰＣＲ），并对照核酸斑点杂交法对１０例口腔恶性肿瘤组织中ＨＰＶ１６Ｅ６转化基因进行了检测。这些肿瘤包括６例口腔粘膜鳞癌（ＳＣＣ），２例涎腺腺样囊性癌（ＡＣＣ），１例涎腺恶性多形性腺瘤（ＭＰＡ）和１例腭部软组织胚胎性横纹肌肉瘤（ＥＲ）。对照组为１０例非肿瘤组织，包括唇裂、腭裂粘膜组织标本各５例。结果表明两种方法检测结果基本一致，肿瘤组阳性６例（６／１０），包括４例ＳＳＣ，１例ＡＣＣ和１例ＥＲ（除外１例ＡＣＣ用核酸杂交法检测为弱阳性），其余为阴性；对照组用核酸杂交法检测４例，除１例唇裂标本为弱阳性以外，均为阴性（０／４）；用ＰＣＲ法检测１０例均为阴性结果（０／１０）。两组间ＨＰＶ１６Ｅ６转化基因的检出率有明显差异（Ｐ＜０．０５）。这一结果为开展口腔癌的ＨＰＶ病因学研究提供了参考资料。     

英破解新流感基因

英国研究人员从欧洲病例样本中破解了甲型H1N1流感病毒的基因密码。     

破译王者基因 梅赛德斯-奔驰S350 4MATIC

传统的观念中，豪华轿车似乎只是城市道路中的穿行者。而随着生活领域的拓展，人们对豪华轿车的操控性以及驾驶乐趣愈加渴望。如今，梅赛德斯-奔驰正在让这一愿望变为现实……     

改进混合遗传算法在车辆路线问题中的应用研究

物流配送车辆路线问题,是物流配送优化中不可缺少的环节.针对传统的遗传算法存在收敛速度慢,局部搜索能力差,易早熟的缺点,采用混合遗传算法进行优化求解.即采用二重结构编码,可以使问题变得更简洁,提高遗传法的搜索效率.用个体数量控制选择策略,以保证群体的多样性,用改进的顺序交叉算子避免优良基因片断在顺序交叉时被破坏,保证算法能够收敛到全局最优.结合具体实例,通过实验计算证明了该改进算法的良好性能.     

基因进化论--大话2013新世代天籁

魏闻博：许洋，你干嘛呢？上班时间别玩游戏，让别人看见了不好。     

赛道基因的天生利器

大众汽车一直致力于推广赛道文化，在不同的市场区域，大众汽车都会加入一些赛事中。早在1976年，大众汽车运动部门在德国汉诺威启动了致力于培养青年赛车手的单一车型杯赛，     

软计算及其新一代模糊控制器

介绍了软计算的概念及构成软件计算的三个组成部分：模糊逻辑、神经网络和概率推理，并讨论了软件为基础的新一代模糊控制器。     

Establishment of a uremic apolipoprotein E knockout mouse model to explore the mechanism of uremic atherosclerosis

Objective To establish a uremic apoE-/- mouse model to observe serum biochemical parameters and features of aortic root atherosclerosis (AS) in the model. Methods A uremic model was induced surgically in apoE-/- mice: electrocautery of the right kidney at 8 weeks of age and nephrectomy (NX) of the left one 2 weeks later. Control mice were sham-operated. Two weeks after NX, renal functions were detected in the uremic and control mice to evaluate the efficiency of the model. After 10 weeks of NX, blood samples were taken to determine serum biochemical parameters, and aortic root was collected for frozen sections to investigate the lesion features of AS. Results Two weeks after NX, renal functions declined significantly in the uremic mice compared with the control ones, and remained stable 8 weeks later either in males or in females. Ten weeks after NX, serum levels of TCH, TG and LDL-C were dramatically higher in the uremic mice than in the controls, whereas no differences in serum HDL-C or glucose concentration were found between the two groups. Aortic root plaque relative area increased significantly in the uremic mice compared with the controls either in males or in females; more-over, the lesion area was larger in female mice than in male ones. Conclusion We established a uremic apoE-/- mouse model successfully, and this model is characterized by accelerated atherogenesis which is associated with an increase in serum lipid profile. This experimental model can be a useful tool to study the pathogenesis and therapeutic strategies of uremic AS.     

槲皮素对体外培养NB4细胞株p53表达的影响

目的 为研究治疗白血病的有效药物。方法 将槲皮素作用于培养的ＮＢ４细胞株，应用ＲＴ－ＰＣＲ及ｗｅｓｔｅｒｎ ｂｌｏｔ方法，研究不同浓度槲皮素（３０、６０、９０μｍ）诱导细胞２４、４８、７２、９６、１２０ｈ后，白血病ＮＢ４细胞株中突变表达产生抑制作用。结论 为槲皮素应用于白血病的研究提供了有效手段。